Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae)
A cytogenetic investigation was performed in eight species of the spittlebug genus Philaenus using silver-NOR (AgNOR)-banding and fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomeric probes. This is the first application of FISH technique in the Auchenorrhyncha, a suborder...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Entomology, Biology Centre, Czech Academy of Science
2013-07-01
|
| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201303-0004_Chromosomal_location_of_rDNA_clusters_and_TTAGG_telomeric_repeats_in_eight_species_of_the_spittlebug_genus_Phil.php |
| _version_ | 1818450704710238208 |
|---|---|
| author | Anna MARYAŃSKA-NADACHOWSKA Valentina G. KUZNETSOVA Tatyana V. KARAMYSHEVA |
| author_facet | Anna MARYAŃSKA-NADACHOWSKA Valentina G. KUZNETSOVA Tatyana V. KARAMYSHEVA |
| author_sort | Anna MARYAŃSKA-NADACHOWSKA |
| collection | DOAJ |
| description | A cytogenetic investigation was performed in eight species of the spittlebug genus Philaenus using silver-NOR (AgNOR)-banding and fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomeric probes. This is the first application of FISH technique in the Auchenorrhyncha, a suborder of the Hemiptera. FISH along with the rDNA probe revealed differences between species in the number and chromosomal location of major ribosomal RNA gene sites, the so-called nucleolar organizer regions (NORs). However, we found a lack of perfect correlation between the results of AgNOR-staining and rDNA-FISH in the detection of NORs. FISH with the telomeric probe confirmed that the chromosome ends of the Philaenus species are composed of the (TTAGG)n nucleotide sequence, which is a common motif of insect telomeres. |
| first_indexed | 2024-12-14T20:55:32Z |
| format | Article |
| id | doaj.art-8efbed6361d745b5b90f0a5a654e2763 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-14T20:55:32Z |
| publishDate | 2013-07-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-8efbed6361d745b5b90f0a5a654e27632022-12-21T22:47:42ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292013-07-01110341141810.14411/eje.2013.055eje-201303-0004Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae)Anna MARYAŃSKA-NADACHOWSKA0Valentina G. KUZNETSOVA1Tatyana V. KARAMYSHEVA2Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Kraków, Poland; e-mail: maryanska@isez.pan.krakow.plZoological Institute of the Russian Academy of Sciences, Saint-Petersburg, Russia; e-mail: karyo@zin.ruInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; e-mail: kary@bionet.nsc.ruA cytogenetic investigation was performed in eight species of the spittlebug genus Philaenus using silver-NOR (AgNOR)-banding and fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomeric probes. This is the first application of FISH technique in the Auchenorrhyncha, a suborder of the Hemiptera. FISH along with the rDNA probe revealed differences between species in the number and chromosomal location of major ribosomal RNA gene sites, the so-called nucleolar organizer regions (NORs). However, we found a lack of perfect correlation between the results of AgNOR-staining and rDNA-FISH in the detection of NORs. FISH with the telomeric probe confirmed that the chromosome ends of the Philaenus species are composed of the (TTAGG)n nucleotide sequence, which is a common motif of insect telomeres.https://www.eje.cz/artkey/eje-201303-0004_Chromosomal_location_of_rDNA_clusters_and_TTAGG_telomeric_repeats_in_eight_species_of_the_spittlebug_genus_Phil.phpphilaenusaphrophoridaeauchenorrhynchafishag-norrdnatelomeres |
| spellingShingle | Anna MARYAŃSKA-NADACHOWSKA Valentina G. KUZNETSOVA Tatyana V. KARAMYSHEVA Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae) European Journal of Entomology philaenus aphrophoridae auchenorrhyncha fish ag-nor rdna telomeres |
| title | Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae) |
| title_full | Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae) |
| title_fullStr | Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae) |
| title_full_unstemmed | Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae) |
| title_short | Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae) |
| title_sort | chromosomal location of rdna clusters and ttagg telomeric repeats in eight species of the spittlebug genus philaenus hemiptera auchenorrhyncha aphrophoridae |
| topic | philaenus aphrophoridae auchenorrhyncha fish ag-nor rdna telomeres |
| url | https://www.eje.cz/artkey/eje-201303-0004_Chromosomal_location_of_rDNA_clusters_and_TTAGG_telomeric_repeats_in_eight_species_of_the_spittlebug_genus_Phil.php |
| work_keys_str_mv | AT annamaryanskanadachowska chromosomallocationofrdnaclustersandttaggtelomericrepeatsineightspeciesofthespittlebuggenusphilaenushemipteraauchenorrhynchaaphrophoridae AT valentinagkuznetsova chromosomallocationofrdnaclustersandttaggtelomericrepeatsineightspeciesofthespittlebuggenusphilaenushemipteraauchenorrhynchaaphrophoridae AT tatyanavkaramysheva chromosomallocationofrdnaclustersandttaggtelomericrepeatsineightspeciesofthespittlebuggenusphilaenushemipteraauchenorrhynchaaphrophoridae |