Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements.
The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different ho...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2004-03-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.0020069 |
| _version_ | 1818936838349389824 |
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| author | Martin Wu Ling V Sun Jessica Vamathevan Markus Riegler Robert Deboy Jeremy C Brownlie Elizabeth A McGraw William Martin Christian Esser Nahal Ahmadinejad Christian Wiegand Ramana Madupu Maureen J Beanan Lauren M Brinkac Sean C Daugherty A Scott Durkin James F Kolonay William C Nelson Yasmin Mohamoud Perris Lee Kristi Berry M Brook Young Teresa Utterback Janice Weidman William C Nierman Ian T Paulsen Karen E Nelson Hervé Tettelin Scott L O'Neill Jonathan A Eisen |
| author_facet | Martin Wu Ling V Sun Jessica Vamathevan Markus Riegler Robert Deboy Jeremy C Brownlie Elizabeth A McGraw William Martin Christian Esser Nahal Ahmadinejad Christian Wiegand Ramana Madupu Maureen J Beanan Lauren M Brinkac Sean C Daugherty A Scott Durkin James F Kolonay William C Nelson Yasmin Mohamoud Perris Lee Kristi Berry M Brook Young Teresa Utterback Janice Weidman William C Nierman Ian T Paulsen Karen E Nelson Hervé Tettelin Scott L O'Neill Jonathan A Eisen |
| author_sort | Martin Wu |
| collection | DOAJ |
| description | The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among sequenced obligate intracellular species in both being highly streamlined and containing very high levels of repetitive DNA and mobile DNA elements. This observation, coupled with multiple evolutionary reconstructions, suggests that natural selection is somewhat inefficient in wMel, most likely owing to the occurrence of repeated population bottlenecks. Genome analysis predicts many metabolic differences with the closely related Rickettsia species, including the presence of intact glycolysis and purine synthesis, which may compensate for an inability to obtain ATP directly from its host, as Rickettsia can. Other discoveries include the apparent inability of wMel to synthesize lipopolysaccharide and the presence of the most genes encoding proteins with ankyrin repeat domains of any prokaryotic genome yet sequenced. Despite the ability of wMel to infect the germline of its host, we find no evidence for either recent lateral gene transfer between wMel and D. melanogaster or older transfers between Wolbachia and any host. Evolutionary analysis further supports the hypothesis that mitochondria share a common ancestor with the alpha-Proteobacteria, but shows little support for the grouping of mitochondria with species in the order Rickettsiales. With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel-D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections. |
| first_indexed | 2024-12-20T05:42:25Z |
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| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-20T05:42:25Z |
| publishDate | 2004-03-01 |
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| series | PLoS Biology |
| spelling | doaj.art-8efc105c227a47e0b3a4d49569ca57c92022-12-21T19:51:25ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852004-03-0123E6910.1371/journal.pbio.0020069Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements.Martin WuLing V SunJessica VamathevanMarkus RieglerRobert DeboyJeremy C BrownlieElizabeth A McGrawWilliam MartinChristian EsserNahal AhmadinejadChristian WiegandRamana MadupuMaureen J BeananLauren M BrinkacSean C DaughertyA Scott DurkinJames F KolonayWilliam C NelsonYasmin MohamoudPerris LeeKristi BerryM Brook YoungTeresa UtterbackJanice WeidmanWilliam C NiermanIan T PaulsenKaren E NelsonHervé TettelinScott L O'NeillJonathan A EisenThe complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among sequenced obligate intracellular species in both being highly streamlined and containing very high levels of repetitive DNA and mobile DNA elements. This observation, coupled with multiple evolutionary reconstructions, suggests that natural selection is somewhat inefficient in wMel, most likely owing to the occurrence of repeated population bottlenecks. Genome analysis predicts many metabolic differences with the closely related Rickettsia species, including the presence of intact glycolysis and purine synthesis, which may compensate for an inability to obtain ATP directly from its host, as Rickettsia can. Other discoveries include the apparent inability of wMel to synthesize lipopolysaccharide and the presence of the most genes encoding proteins with ankyrin repeat domains of any prokaryotic genome yet sequenced. Despite the ability of wMel to infect the germline of its host, we find no evidence for either recent lateral gene transfer between wMel and D. melanogaster or older transfers between Wolbachia and any host. Evolutionary analysis further supports the hypothesis that mitochondria share a common ancestor with the alpha-Proteobacteria, but shows little support for the grouping of mitochondria with species in the order Rickettsiales. With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel-D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections.https://doi.org/10.1371/journal.pbio.0020069 |
| spellingShingle | Martin Wu Ling V Sun Jessica Vamathevan Markus Riegler Robert Deboy Jeremy C Brownlie Elizabeth A McGraw William Martin Christian Esser Nahal Ahmadinejad Christian Wiegand Ramana Madupu Maureen J Beanan Lauren M Brinkac Sean C Daugherty A Scott Durkin James F Kolonay William C Nelson Yasmin Mohamoud Perris Lee Kristi Berry M Brook Young Teresa Utterback Janice Weidman William C Nierman Ian T Paulsen Karen E Nelson Hervé Tettelin Scott L O'Neill Jonathan A Eisen Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. PLoS Biology |
| title | Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. |
| title_full | Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. |
| title_fullStr | Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. |
| title_full_unstemmed | Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. |
| title_short | Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. |
| title_sort | phylogenomics of the reproductive parasite wolbachia pipientis wmel a streamlined genome overrun by mobile genetic elements |
| url | https://doi.org/10.1371/journal.pbio.0020069 |
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