| Summary: | The present study aims at valorizing Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical analysis of <i>Henna</i> leaves was performed by determining phenolic compound contents, and flavonoids and tannins in it, through its aqueous extracts. Then, specific formulations were prepared using aqueous extracts of <i>L. inermis</i> to assess their in vivo wound healing potential in Swiss albino mice used as animal models. Results disclosed that phenolic compounds (13.48%), as well as flavonoid (9.25%) and tannin (2.57%) contents are higher in <i>Henna</i> leaf extracts from Alnif, while Tazarine <i>Henna</i> aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Sephadex G50 gel corroborates the obtained results and shows that Lawsone levels (<i>Henna</i> coloring agent) are higher in <i>Henna</i> collected from Alnif. Aqueous <i>Henna</i> leaf extracts, at a dose of 10% in petroleum jelly, have been assessed for their ability to heal induced burns in mice. Healing monitoring, carried-out with <i>Henna</i> extracts on mice batches and those of two control batches (mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% (flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the 3 studied <i>Henna</i>-based formulations. These findings suggest the interest of potential development of <i>Henna</i>-based formulations, as a source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications.
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