Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts
The present study aims at valorizing Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Taza...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/10/3/92 |
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| author | Soukaina El Massoudi Abdellah Zinedine João Miguel Rocha Meryem Benidir Ilham Najjari Lahsen El Ghadraoui Meryem Benjelloun Faouzi Errachidi |
| author_facet | Soukaina El Massoudi Abdellah Zinedine João Miguel Rocha Meryem Benidir Ilham Najjari Lahsen El Ghadraoui Meryem Benjelloun Faouzi Errachidi |
| author_sort | Soukaina El Massoudi |
| collection | DOAJ |
| description | The present study aims at valorizing Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical analysis of <i>Henna</i> leaves was performed by determining phenolic compound contents, and flavonoids and tannins in it, through its aqueous extracts. Then, specific formulations were prepared using aqueous extracts of <i>L. inermis</i> to assess their in vivo wound healing potential in Swiss albino mice used as animal models. Results disclosed that phenolic compounds (13.48%), as well as flavonoid (9.25%) and tannin (2.57%) contents are higher in <i>Henna</i> leaf extracts from Alnif, while Tazarine <i>Henna</i> aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Sephadex G50 gel corroborates the obtained results and shows that Lawsone levels (<i>Henna</i> coloring agent) are higher in <i>Henna</i> collected from Alnif. Aqueous <i>Henna</i> leaf extracts, at a dose of 10% in petroleum jelly, have been assessed for their ability to heal induced burns in mice. Healing monitoring, carried-out with <i>Henna</i> extracts on mice batches and those of two control batches (mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% (flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the 3 studied <i>Henna</i>-based formulations. These findings suggest the interest of potential development of <i>Henna</i>-based formulations, as a source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications. |
| first_indexed | 2024-03-11T02:35:56Z |
| format | Article |
| id | doaj.art-8efdcd8486fb485f898fe35a644a6e85 |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-11T02:35:56Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-8efdcd8486fb485f898fe35a644a6e852023-11-18T09:55:13ZengMDPI AGCosmetics2079-92842023-06-011039210.3390/cosmetics10030092Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous ExtractsSoukaina El Massoudi0Abdellah Zinedine1João Miguel Rocha2Meryem Benidir3Ilham Najjari4Lahsen El Ghadraoui5Meryem Benjelloun6Faouzi Errachidi7Functional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30050, MoroccoBIOMARE Laboratory, Faculty of Sciences, Chouaib Doukkali University, P.O. Box 20, EL Jadida 24000, MoroccoCBQF-Centro de Biotecnologia e Química Fina–Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalFunctional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30050, MoroccoFunctional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30050, MoroccoFunctional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30050, MoroccoFunctional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30050, MoroccoFunctional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30050, MoroccoThe present study aims at valorizing Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical analysis of <i>Henna</i> leaves was performed by determining phenolic compound contents, and flavonoids and tannins in it, through its aqueous extracts. Then, specific formulations were prepared using aqueous extracts of <i>L. inermis</i> to assess their in vivo wound healing potential in Swiss albino mice used as animal models. Results disclosed that phenolic compounds (13.48%), as well as flavonoid (9.25%) and tannin (2.57%) contents are higher in <i>Henna</i> leaf extracts from Alnif, while Tazarine <i>Henna</i> aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Sephadex G50 gel corroborates the obtained results and shows that Lawsone levels (<i>Henna</i> coloring agent) are higher in <i>Henna</i> collected from Alnif. Aqueous <i>Henna</i> leaf extracts, at a dose of 10% in petroleum jelly, have been assessed for their ability to heal induced burns in mice. Healing monitoring, carried-out with <i>Henna</i> extracts on mice batches and those of two control batches (mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% (flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the 3 studied <i>Henna</i>-based formulations. These findings suggest the interest of potential development of <i>Henna</i>-based formulations, as a source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications.https://www.mdpi.com/2079-9284/10/3/92<i>Lawsonia inermis</i>Hennaleavesphytochemicalwound healing activityMorocco |
| spellingShingle | Soukaina El Massoudi Abdellah Zinedine João Miguel Rocha Meryem Benidir Ilham Najjari Lahsen El Ghadraoui Meryem Benjelloun Faouzi Errachidi Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts Cosmetics <i>Lawsonia inermis</i> Henna leaves phytochemical wound healing activity Morocco |
| title | Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts |
| title_full | Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts |
| title_fullStr | Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts |
| title_full_unstemmed | Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts |
| title_short | Phenolic Composition and Wound Healing Potential Assessment of Moroccan <i>Henna</i> (<i>Lawsonia inermis</i>) Aqueous Extracts |
| title_sort | phenolic composition and wound healing potential assessment of moroccan i henna i i lawsonia inermis i aqueous extracts |
| topic | <i>Lawsonia inermis</i> Henna leaves phytochemical wound healing activity Morocco |
| url | https://www.mdpi.com/2079-9284/10/3/92 |
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