The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium
BackgroundThe in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid (AA) blend (tryptophan, threonine, and methionine) in pigs.ObjectiveThis study aimed to evaluate the in vitro anti-inflammatory and anti-oxidative effects of an AA blend on intestinal porcine epithelial...
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| Format: | Article |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1367328/full |
| _version_ | 1797263581776969728 |
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| author | Sehyeong Ham Jeongmin Suh Jieun Kim Min Jeong Gu Min Ah Park Eunseon Oh Jun-Ok Moon Chanhee Chae |
| author_facet | Sehyeong Ham Jeongmin Suh Jieun Kim Min Jeong Gu Min Ah Park Eunseon Oh Jun-Ok Moon Chanhee Chae |
| author_sort | Sehyeong Ham |
| collection | DOAJ |
| description | BackgroundThe in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid (AA) blend (tryptophan, threonine, and methionine) in pigs.ObjectiveThis study aimed to evaluate the in vitro anti-inflammatory and anti-oxidative effects of an AA blend on intestinal porcine epithelial cells (IPEC-J2) and the in vivo anti-inflammatory and anti-oxidative effects in pigs experimentally challenged with Salmonella Typhimurium.MethodsIPEC-J2 were pretreated with an AA blend for 25 h and then treated with lipopolysaccharide (LPS), deoxynivalenol (DON), or H2O2 for in vitro evaluation. A controlled standard diet supplemented with 0.3% of the AA blend was orally fed to the treated group pigs for 14 days, beginning at 21 days of age. At the end of the feeding period, pigs were orally inoculated with Salmonella Typhimurium.ResultsPre-treatment with the AA blend reduced LPS/DON-induced interleukin (IL)-8 mRNA as a measurement of the anti-inflammatory effect and H2O2-induced reactive oxygen species (ROS) as a measurement of the anti-oxidative effect on IPEC-J2. Feeding with an AA blend resulted in a reduction of proinflammatory (tumor necrosis factor-α, IL-6, and IL-8) cytokine levels, while treated pigs experienced an increase in anti-inflammatory IL-10 cytokine in their sera. The addition of an AA blend-supplemented pig feed resulted in significantly lower Salmonella-induced cecal lesion scores compared to untreated pigs.DiscussionSupplementation of feed with an AA blend reduced intestinal inflammation and pathology in pigs and may be applied for the control of Salmonella Typhimurium infection, as demonstrated in this study. |
| first_indexed | 2024-04-25T00:15:17Z |
| format | Article |
| id | doaj.art-8f02a50f226e4d7fa9aa69b3660e34e5 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-25T00:15:17Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-8f02a50f226e4d7fa9aa69b3660e34e52024-03-13T04:53:52ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-03-011110.3389/fvets.2024.13673281367328The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella TyphimuriumSehyeong Ham0Jeongmin Suh1Jieun Kim2Min Jeong Gu3Min Ah Park4Eunseon Oh5Jun-Ok Moon6Chanhee Chae7Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaApplication Center, CJ Blossom Park, Suwon, Republic of KoreaApplication Center, CJ Blossom Park, Suwon, Republic of KoreaApplication Center, CJ Blossom Park, Suwon, Republic of KoreaApplication Center, CJ Blossom Park, Suwon, Republic of KoreaApplication Center, CJ Blossom Park, Suwon, Republic of KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of KoreaBackgroundThe in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid (AA) blend (tryptophan, threonine, and methionine) in pigs.ObjectiveThis study aimed to evaluate the in vitro anti-inflammatory and anti-oxidative effects of an AA blend on intestinal porcine epithelial cells (IPEC-J2) and the in vivo anti-inflammatory and anti-oxidative effects in pigs experimentally challenged with Salmonella Typhimurium.MethodsIPEC-J2 were pretreated with an AA blend for 25 h and then treated with lipopolysaccharide (LPS), deoxynivalenol (DON), or H2O2 for in vitro evaluation. A controlled standard diet supplemented with 0.3% of the AA blend was orally fed to the treated group pigs for 14 days, beginning at 21 days of age. At the end of the feeding period, pigs were orally inoculated with Salmonella Typhimurium.ResultsPre-treatment with the AA blend reduced LPS/DON-induced interleukin (IL)-8 mRNA as a measurement of the anti-inflammatory effect and H2O2-induced reactive oxygen species (ROS) as a measurement of the anti-oxidative effect on IPEC-J2. Feeding with an AA blend resulted in a reduction of proinflammatory (tumor necrosis factor-α, IL-6, and IL-8) cytokine levels, while treated pigs experienced an increase in anti-inflammatory IL-10 cytokine in their sera. The addition of an AA blend-supplemented pig feed resulted in significantly lower Salmonella-induced cecal lesion scores compared to untreated pigs.DiscussionSupplementation of feed with an AA blend reduced intestinal inflammation and pathology in pigs and may be applied for the control of Salmonella Typhimurium infection, as demonstrated in this study.https://www.frontiersin.org/articles/10.3389/fvets.2024.1367328/fullamino acid blendcytokineinflammationSalmonella Typhimuriumporcine |
| spellingShingle | Sehyeong Ham Jeongmin Suh Jieun Kim Min Jeong Gu Min Ah Park Eunseon Oh Jun-Ok Moon Chanhee Chae The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium Frontiers in Veterinary Science amino acid blend cytokine inflammation Salmonella Typhimurium porcine |
| title | The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium |
| title_full | The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium |
| title_fullStr | The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium |
| title_full_unstemmed | The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium |
| title_short | The in vitro and in vivo anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with Salmonella Typhimurium |
| title_sort | in vitro and in vivo anti inflammatory and anti oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with salmonella typhimurium |
| topic | amino acid blend cytokine inflammation Salmonella Typhimurium porcine |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1367328/full |
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