Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh
<i>Escherichia coli</i> is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing <i>E. coli</i> (ESBL-...
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MDPI AG
2020-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/6/420 |
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| author | Mst. Sonia Parvin Sudipta Talukder Md. Yamin Ali Emdadul Haque Chowdhury Md. Tanvir Rahman Md. Taohidul Islam |
| author_facet | Mst. Sonia Parvin Sudipta Talukder Md. Yamin Ali Emdadul Haque Chowdhury Md. Tanvir Rahman Md. Taohidul Islam |
| author_sort | Mst. Sonia Parvin |
| collection | DOAJ |
| description | <i>Escherichia coli</i> is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing <i>E. coli</i> (ESBL-Ec), in particular, in chicken meat is considered a global health problem. This study aimed to determine the magnitude of <i>E. coli</i>, with special emphasis on ESBL-Ec, along with their phenotypic antimicrobial resistance pattern in frozen chicken meat. The study also focused on the determination of ESBL-encoding genes in <i>E. coli</i>. A total of 113 frozen chicken meat samples were purchased from 40 outlets of nine branded supershops in five megacities in Bangladesh. Isolation and identification of <i>E. coli</i> were done based on cultural and biochemical properties, as well as PCR assay. The resistance pattern was determined by the disc diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% of samples were positive for <i>E. coli</i>, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9–11 and 12–13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively, while only 11.6% were resistant to 3–5 classes. Possible extensive drug resistance (pXDR) was found in 2.3% of isolates. High single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim–sulfamethoxazole, and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for the <i>bla</i>TEM gene. However, the <i>bla</i>SHV and <i>bla</i>CTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates carried the <i>bla</i>CTX-M-1 gene. This study provided evidence of the existence of MDR and pXDR ESBL-Ec in frozen chicken meat in Bangladesh, which may pose a risk to human health if the meat is not properly cooked or pickled raw only. This emphasizes the importance of the implementation of good slaughtering and processing practices by the processors. |
| first_indexed | 2024-03-10T19:31:40Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-10T19:31:40Z |
| publishDate | 2020-05-01 |
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| spelling | doaj.art-8f0639327f564b69a69aef6c0cb8b4fc2023-11-20T02:03:21ZengMDPI AGPathogens2076-08172020-05-019642010.3390/pathogens9060420Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in BangladeshMst. Sonia Parvin0Sudipta Talukder1Md. Yamin Ali2Emdadul Haque Chowdhury3Md. Tanvir Rahman4Md. Taohidul Islam5Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh<i>Escherichia coli</i> is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing <i>E. coli</i> (ESBL-Ec), in particular, in chicken meat is considered a global health problem. This study aimed to determine the magnitude of <i>E. coli</i>, with special emphasis on ESBL-Ec, along with their phenotypic antimicrobial resistance pattern in frozen chicken meat. The study also focused on the determination of ESBL-encoding genes in <i>E. coli</i>. A total of 113 frozen chicken meat samples were purchased from 40 outlets of nine branded supershops in five megacities in Bangladesh. Isolation and identification of <i>E. coli</i> were done based on cultural and biochemical properties, as well as PCR assay. The resistance pattern was determined by the disc diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% of samples were positive for <i>E. coli</i>, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9–11 and 12–13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively, while only 11.6% were resistant to 3–5 classes. Possible extensive drug resistance (pXDR) was found in 2.3% of isolates. High single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim–sulfamethoxazole, and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for the <i>bla</i>TEM gene. However, the <i>bla</i>SHV and <i>bla</i>CTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates carried the <i>bla</i>CTX-M-1 gene. This study provided evidence of the existence of MDR and pXDR ESBL-Ec in frozen chicken meat in Bangladesh, which may pose a risk to human health if the meat is not properly cooked or pickled raw only. This emphasizes the importance of the implementation of good slaughtering and processing practices by the processors.https://www.mdpi.com/2076-0817/9/6/420<i>Escherichia coli</i>antimicrobial resistanceESBLMDRfrozen chicken meatBangladesh |
| spellingShingle | Mst. Sonia Parvin Sudipta Talukder Md. Yamin Ali Emdadul Haque Chowdhury Md. Tanvir Rahman Md. Taohidul Islam Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh Pathogens <i>Escherichia coli</i> antimicrobial resistance ESBL MDR frozen chicken meat Bangladesh |
| title | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_full | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_fullStr | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_full_unstemmed | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_short | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolated from Frozen Chicken Meat in Bangladesh |
| title_sort | antimicrobial resistance pattern of i escherichia coli i isolated from frozen chicken meat in bangladesh |
| topic | <i>Escherichia coli</i> antimicrobial resistance ESBL MDR frozen chicken meat Bangladesh |
| url | https://www.mdpi.com/2076-0817/9/6/420 |
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