Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice.
Unrepaired or misrepaired DNA damage has been implicated as a causal factor in cancer and aging. Xpd(TTD) mice, harboring defects in nucleotide excision repair and transcription due to a mutation in the Xpd gene (R722W), display severe symptoms of premature aging but have a reduced incidence of canc...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-06-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2396506?pdf=render |
| _version_ | 1811322172960931840 |
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| author | Jung Yoon Park Mi-Ook Cho Shanique Leonard Brent Calder I Saira Mian Woo Ho Kim Susan Wijnhoven Harry van Steeg James Mitchell Gijsbertus T J van der Horst Jan Hoeijmakers Pinchas Cohen Jan Vijg Yousin Suh |
| author_facet | Jung Yoon Park Mi-Ook Cho Shanique Leonard Brent Calder I Saira Mian Woo Ho Kim Susan Wijnhoven Harry van Steeg James Mitchell Gijsbertus T J van der Horst Jan Hoeijmakers Pinchas Cohen Jan Vijg Yousin Suh |
| author_sort | Jung Yoon Park |
| collection | DOAJ |
| description | Unrepaired or misrepaired DNA damage has been implicated as a causal factor in cancer and aging. Xpd(TTD) mice, harboring defects in nucleotide excision repair and transcription due to a mutation in the Xpd gene (R722W), display severe symptoms of premature aging but have a reduced incidence of cancer. To gain further insight into the molecular basis of the mutant-specific manifestation of age-related phenotypes, we used comparative microarray analysis of young and old female livers to discover gene expression signatures distinguishing Xpd(TTD) mice from their age-matched wild type controls. We found a transcription signature of increased apoptosis in the Xpd(TTD) mice, which was confirmed by in situ immunohistochemical analysis and found to be accompanied by increased proliferation. However, apoptosis rate exceeded the rate of proliferation, resulting in homeostatic imbalance. Interestingly, a metabolic response signature was observed involving decreased energy metabolism and reduced IGF-1 signaling, a major modulator of life span. We conclude that while the increased apoptotic response to endogenous DNA damage contributes to the accelerated aging phenotypes and the reduced cancer incidence observed in the Xpd(TTD) mice, the signature of reduced energy metabolism is likely to reflect a compensatory adjustment to limit the increased genotoxic stress in these mutants. These results support a general model for premature aging in DNA repair deficient mice based on cellular responses to DNA damage that impair normal tissue homeostasis. |
| first_indexed | 2024-04-13T13:30:36Z |
| format | Article |
| id | doaj.art-8f121145a76c467c89138853ded00299 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:30:36Z |
| publishDate | 2008-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8f121145a76c467c89138853ded002992022-12-22T02:44:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-06-0136e234610.1371/journal.pone.0002346Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice.Jung Yoon ParkMi-Ook ChoShanique LeonardBrent CalderI Saira MianWoo Ho KimSusan WijnhovenHarry van SteegJames MitchellGijsbertus T J van der HorstJan HoeijmakersPinchas CohenJan VijgYousin SuhUnrepaired or misrepaired DNA damage has been implicated as a causal factor in cancer and aging. Xpd(TTD) mice, harboring defects in nucleotide excision repair and transcription due to a mutation in the Xpd gene (R722W), display severe symptoms of premature aging but have a reduced incidence of cancer. To gain further insight into the molecular basis of the mutant-specific manifestation of age-related phenotypes, we used comparative microarray analysis of young and old female livers to discover gene expression signatures distinguishing Xpd(TTD) mice from their age-matched wild type controls. We found a transcription signature of increased apoptosis in the Xpd(TTD) mice, which was confirmed by in situ immunohistochemical analysis and found to be accompanied by increased proliferation. However, apoptosis rate exceeded the rate of proliferation, resulting in homeostatic imbalance. Interestingly, a metabolic response signature was observed involving decreased energy metabolism and reduced IGF-1 signaling, a major modulator of life span. We conclude that while the increased apoptotic response to endogenous DNA damage contributes to the accelerated aging phenotypes and the reduced cancer incidence observed in the Xpd(TTD) mice, the signature of reduced energy metabolism is likely to reflect a compensatory adjustment to limit the increased genotoxic stress in these mutants. These results support a general model for premature aging in DNA repair deficient mice based on cellular responses to DNA damage that impair normal tissue homeostasis.http://europepmc.org/articles/PMC2396506?pdf=render |
| spellingShingle | Jung Yoon Park Mi-Ook Cho Shanique Leonard Brent Calder I Saira Mian Woo Ho Kim Susan Wijnhoven Harry van Steeg James Mitchell Gijsbertus T J van der Horst Jan Hoeijmakers Pinchas Cohen Jan Vijg Yousin Suh Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice. PLoS ONE |
| title | Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice. |
| title_full | Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice. |
| title_fullStr | Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice. |
| title_full_unstemmed | Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice. |
| title_short | Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice. |
| title_sort | homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging xpd mice |
| url | http://europepmc.org/articles/PMC2396506?pdf=render |
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