Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study
Background: Human milk oligosaccharides are complex, non-digestible carbohydrates that directly interact with intestinal epithelial cells to alter barrier function and host inflammation. Oligosaccharide composition varies widely between individual mothers, but it is unclear if this inter-individual...
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MDPI AG
2022-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/5/1014 |
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| author | Richard Y. Wu Steven R. Botts Kathene C. Johnson-Henry Eva Landberg Thomas R. Abrahamsson Philip M. Sherman |
| author_facet | Richard Y. Wu Steven R. Botts Kathene C. Johnson-Henry Eva Landberg Thomas R. Abrahamsson Philip M. Sherman |
| author_sort | Richard Y. Wu |
| collection | DOAJ |
| description | Background: Human milk oligosaccharides are complex, non-digestible carbohydrates that directly interact with intestinal epithelial cells to alter barrier function and host inflammation. Oligosaccharide composition varies widely between individual mothers, but it is unclear if this inter-individual variation has any impact on intestinal epithelial barrier function and gut inflammation. Methods: Human milk oligosaccharides were extracted from the mature human milk of four individual donors. Using an in vitro model of intestinal injury, the effects of the oligosaccharides on the intestinal epithelial barrier and select innate and adaptive immune functions were assessed. Results: Individual oligosaccharide compositions shared comparable effects on increasing transepithelial electrical resistance and reducing the macromolecular permeability of polarized (Caco-2Bbe1) monolayers but exerted distinct effects on the localization of the intercellular tight junction protein zona occludins-1 in response to injury induced by a human enteric bacterial pathogen <i>Escherichia coli</i>, serotype O157:H7. Immunoblots showed the differential effects of oligosaccharide compositions in reducing host chemokine interleukin 8 expression and inhibiting of p38 MAP kinase activation. Conclusions: These results provide evidence of both shared and distinct effects on the host intestinal epithelial function that are attributable to inter-individual differences in the composition of human milk oligosaccharides. |
| first_indexed | 2024-03-09T20:27:21Z |
| format | Article |
| id | doaj.art-8f13e171c5964c0b96a25f6120f124e5 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T20:27:21Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-8f13e171c5964c0b96a25f6120f124e52023-11-23T23:33:12ZengMDPI AGNutrients2072-66432022-02-01145101410.3390/nu14051014Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot StudyRichard Y. Wu0Steven R. Botts1Kathene C. Johnson-Henry2Eva Landberg3Thomas R. Abrahamsson4Philip M. Sherman5Cell Biology Program, Research Institute Hospital for Sick Children, Toronto, ON M5G 0A4, CanadaCell Biology Program, Research Institute Hospital for Sick Children, Toronto, ON M5G 0A4, CanadaCell Biology Program, Research Institute Hospital for Sick Children, Toronto, ON M5G 0A4, CanadaDepartment of Clinical Chemistry, Linköping University, 58183 Linköping, Östergötland, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58183 Linköping, Östergötland, SwedenCell Biology Program, Research Institute Hospital for Sick Children, Toronto, ON M5G 0A4, CanadaBackground: Human milk oligosaccharides are complex, non-digestible carbohydrates that directly interact with intestinal epithelial cells to alter barrier function and host inflammation. Oligosaccharide composition varies widely between individual mothers, but it is unclear if this inter-individual variation has any impact on intestinal epithelial barrier function and gut inflammation. Methods: Human milk oligosaccharides were extracted from the mature human milk of four individual donors. Using an in vitro model of intestinal injury, the effects of the oligosaccharides on the intestinal epithelial barrier and select innate and adaptive immune functions were assessed. Results: Individual oligosaccharide compositions shared comparable effects on increasing transepithelial electrical resistance and reducing the macromolecular permeability of polarized (Caco-2Bbe1) monolayers but exerted distinct effects on the localization of the intercellular tight junction protein zona occludins-1 in response to injury induced by a human enteric bacterial pathogen <i>Escherichia coli</i>, serotype O157:H7. Immunoblots showed the differential effects of oligosaccharide compositions in reducing host chemokine interleukin 8 expression and inhibiting of p38 MAP kinase activation. Conclusions: These results provide evidence of both shared and distinct effects on the host intestinal epithelial function that are attributable to inter-individual differences in the composition of human milk oligosaccharides.https://www.mdpi.com/2072-6643/14/5/1014inflammationhuman milk oligosaccharidesintestinal epithelial barrier |
| spellingShingle | Richard Y. Wu Steven R. Botts Kathene C. Johnson-Henry Eva Landberg Thomas R. Abrahamsson Philip M. Sherman Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study Nutrients inflammation human milk oligosaccharides intestinal epithelial barrier |
| title | Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study |
| title_full | Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study |
| title_fullStr | Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study |
| title_full_unstemmed | Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study |
| title_short | Variations in the Composition of Human Milk Oligosaccharides Correlates with Effects on Both the Intestinal Epithelial Barrier and Host Inflammation: A Pilot Study |
| title_sort | variations in the composition of human milk oligosaccharides correlates with effects on both the intestinal epithelial barrier and host inflammation a pilot study |
| topic | inflammation human milk oligosaccharides intestinal epithelial barrier |
| url | https://www.mdpi.com/2072-6643/14/5/1014 |
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