Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2
Synthesis of anticancer substances and studying their binding abilities towards human serum proteins as carriers are important parts of pharmaceutical and medical sciences development. The presented work is a continuation of studies of quinobenzothiazine derivatives binding with serum proteins. The...
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MDPI AG
2023-01-01
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| author | Aleksandra Owczarzy Wojciech Rogóż Karolina Kulig Jadwiga Pożycka Andrzej Zięba Małgorzata Maciążek-Jurczyk |
| author_facet | Aleksandra Owczarzy Wojciech Rogóż Karolina Kulig Jadwiga Pożycka Andrzej Zięba Małgorzata Maciążek-Jurczyk |
| author_sort | Aleksandra Owczarzy |
| collection | DOAJ |
| description | Synthesis of anticancer substances and studying their binding abilities towards human serum proteins as carriers are important parts of pharmaceutical and medical sciences development. The presented work is a continuation of studies of quinobenzothiazine derivatives binding with serum proteins. The main aim of this work was a spectroscopic analysis of second from benzothiazinium derivatives salt, 9-fluoro-5-alkyl-12(H)-quino [3,4-b][1,4]benzothiazinium chloride (Salt2), its interaction with carrier proteins, i.e., human serum albumin (HSA), α<sub>1</sub>-acid glycoprotein (AGP), human gamma globulin (HGG), and the study of protein secondary and tertiary structure changes using spectroscopic techniques (spectrofluorescence, UV-Vis and circular dichroism CD spectroscopy). In order to mimic in vivo conditions, control normal serum (CNS) was used. Using the Klotz method, both binding constants (K<sub>a</sub> [M<sup>−1</sup>]) and the number of binding classes (n) were calculated. In addition, the percentage of displacement of binding site markers from HSA and AGP molecules has been defined. Based on the obtained data, it can be concluded that the main binding protein for Salt2 is AGP. HSA and HGG are also involved in the distribution of the studied substance in the bloodstream. Moreover, Salt2 very slightly interacts with CNS, which can cause strong therapeutic as well as toxic effects. The analysis of CD spectra confirms that there are no changes in the secondary structure of the main binding proteins in the presence of Salt2. |
| first_indexed | 2024-03-09T11:35:06Z |
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| id | doaj.art-8f152f2cdba642099feca0f06a6789a8 |
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| language | English |
| last_indexed | 2024-03-09T11:35:06Z |
| publishDate | 2023-01-01 |
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| series | Molecules |
| spelling | doaj.art-8f152f2cdba642099feca0f06a6789a82023-11-30T23:43:30ZengMDPI AGMolecules1420-30492023-01-0128269810.3390/molecules28020698Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2Aleksandra Owczarzy0Wojciech Rogóż1Karolina Kulig2Jadwiga Pożycka3Andrzej Zięba4Małgorzata Maciążek-Jurczyk5Department of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Organic Chemistry, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandSynthesis of anticancer substances and studying their binding abilities towards human serum proteins as carriers are important parts of pharmaceutical and medical sciences development. The presented work is a continuation of studies of quinobenzothiazine derivatives binding with serum proteins. The main aim of this work was a spectroscopic analysis of second from benzothiazinium derivatives salt, 9-fluoro-5-alkyl-12(H)-quino [3,4-b][1,4]benzothiazinium chloride (Salt2), its interaction with carrier proteins, i.e., human serum albumin (HSA), α<sub>1</sub>-acid glycoprotein (AGP), human gamma globulin (HGG), and the study of protein secondary and tertiary structure changes using spectroscopic techniques (spectrofluorescence, UV-Vis and circular dichroism CD spectroscopy). In order to mimic in vivo conditions, control normal serum (CNS) was used. Using the Klotz method, both binding constants (K<sub>a</sub> [M<sup>−1</sup>]) and the number of binding classes (n) were calculated. In addition, the percentage of displacement of binding site markers from HSA and AGP molecules has been defined. Based on the obtained data, it can be concluded that the main binding protein for Salt2 is AGP. HSA and HGG are also involved in the distribution of the studied substance in the bloodstream. Moreover, Salt2 very slightly interacts with CNS, which can cause strong therapeutic as well as toxic effects. The analysis of CD spectra confirms that there are no changes in the secondary structure of the main binding proteins in the presence of Salt2.https://www.mdpi.com/1420-3049/28/2/698plasma proteins5-alkyl-12(H)-quino [3,4-b][1,4]benzothiazinium derivativefluorescence spectroscopyUV-vis spectroscopycircular dichroism |
| spellingShingle | Aleksandra Owczarzy Wojciech Rogóż Karolina Kulig Jadwiga Pożycka Andrzej Zięba Małgorzata Maciążek-Jurczyk Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2 Molecules plasma proteins 5-alkyl-12(H)-quino [3,4-b][1,4]benzothiazinium derivative fluorescence spectroscopy UV-vis spectroscopy circular dichroism |
| title | Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2 |
| title_full | Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2 |
| title_fullStr | Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2 |
| title_full_unstemmed | Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2 |
| title_short | Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins: Part 2 |
| title_sort | spectroscopic studies of quinobenzothiazine derivative in terms of the in vitro interaction with selected human plasma proteins part 2 |
| topic | plasma proteins 5-alkyl-12(H)-quino [3,4-b][1,4]benzothiazinium derivative fluorescence spectroscopy UV-vis spectroscopy circular dichroism |
| url | https://www.mdpi.com/1420-3049/28/2/698 |
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