Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro
Abstract: This study tested a novel in vitro dental erosion-abrasion model and the performance of cross-polarization optical coherence tomography (CP-OCT) in longitudinally monitoring the simulated lesions. Thirty human enamel specimens were prepared and randomized to receive three dental erosion-ab...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Pesquisa Odontológica
2023-09-01
|
| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100265&lng=en&tlng=en |
| _version_ | 1827824738500608000 |
|---|---|
| author | Maria Jacinta Rosario Romero Sávio José Cardoso Bezerra Daniel Fried Frank Lippert George Joseph Eckert Anderson Takeo Hara |
| author_facet | Maria Jacinta Rosario Romero Sávio José Cardoso Bezerra Daniel Fried Frank Lippert George Joseph Eckert Anderson Takeo Hara |
| author_sort | Maria Jacinta Rosario Romero |
| collection | DOAJ |
| description | Abstract: This study tested a novel in vitro dental erosion-abrasion model and the performance of cross-polarization optical coherence tomography (CP-OCT) in longitudinally monitoring the simulated lesions. Thirty human enamel specimens were prepared and randomized to receive three dental erosion-abrasion (EA) protocols: severe (s-EA, lemon juice/pH:2.5/4.25%w/v citric acid), moderate (m-EA, grapefruit juice/pH:3.5/1.03%w/v citric acid) and no-EA (water, control). EA challenge was performed by exposing the specimens to acidic solutions 4x/day and to brushing 2x/day with 1:3 fluoridated toothpaste slurry, for 14 days. Enamel thickness measurements were obtained using CP-OCT at baseline (D0), 7 (D7) and 14 days (D14) and micro-computed tomography (micro-CT) at D14. Enamel surface loss was measured with both CP-OCT and optical profilometry at D0, D7 and D14. Data was analyzed with repeated-measures ANOVA and Pearson's correlation (r) (α = 0.05). CP-OCT enamel thickness decreased over time in the s-EA group (D0 >D7 > D14, p < 0.001) and m-EA group (D0 > D14, p = 0.019) but did not change in the no-EA group (p = 0.30). Overall, CP-OCT and micro-CT results at D14 correlated moderately (r = 0.73). CP-OCT surface loss was highest for s-EA (p <0.001) but did not differ between moderate and no-EA (p = 0.25). Enamel surface loss with profilometry increased with severity (no-EA>m-EA>s-EA, p < 0.001). D14 surface loss was higher than D7 for both methods except for the no-EA group with profilometry. CP-OCT and profilometry had moderate overall correlation (r = 0.70). Our results revealed that the currently proposed in vitro dental erosion-abrasion model is valid and could simulate lesions of different severities over time. CP-OCT was a suitable method for monitoring the EA lesions. |
| first_indexed | 2024-03-12T02:32:02Z |
| format | Article |
| id | doaj.art-8f1687c3c294480286a08430056d4246 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-03-12T02:32:02Z |
| publishDate | 2023-09-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-8f1687c3c294480286a08430056d42462023-09-05T07:43:59ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072023-09-013710.1590/1807-3107bor-2023.vol37.0081Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitroMaria Jacinta Rosario Romerohttps://orcid.org/0000-0002-1263-9931Sávio José Cardoso Bezerrahttps://orcid.org/0000-0002-6273-0426Daniel Friedhttps://orcid.org/0000-0002-5327-2558Frank Lipperthttps://orcid.org/0000-0003-1944-2960George Joseph Eckerthttps://orcid.org/0000-0001-7798-7155Anderson Takeo Harahttps://orcid.org/0000-0001-9822-6064Abstract: This study tested a novel in vitro dental erosion-abrasion model and the performance of cross-polarization optical coherence tomography (CP-OCT) in longitudinally monitoring the simulated lesions. Thirty human enamel specimens were prepared and randomized to receive three dental erosion-abrasion (EA) protocols: severe (s-EA, lemon juice/pH:2.5/4.25%w/v citric acid), moderate (m-EA, grapefruit juice/pH:3.5/1.03%w/v citric acid) and no-EA (water, control). EA challenge was performed by exposing the specimens to acidic solutions 4x/day and to brushing 2x/day with 1:3 fluoridated toothpaste slurry, for 14 days. Enamel thickness measurements were obtained using CP-OCT at baseline (D0), 7 (D7) and 14 days (D14) and micro-computed tomography (micro-CT) at D14. Enamel surface loss was measured with both CP-OCT and optical profilometry at D0, D7 and D14. Data was analyzed with repeated-measures ANOVA and Pearson's correlation (r) (α = 0.05). CP-OCT enamel thickness decreased over time in the s-EA group (D0 >D7 > D14, p < 0.001) and m-EA group (D0 > D14, p = 0.019) but did not change in the no-EA group (p = 0.30). Overall, CP-OCT and micro-CT results at D14 correlated moderately (r = 0.73). CP-OCT surface loss was highest for s-EA (p <0.001) but did not differ between moderate and no-EA (p = 0.25). Enamel surface loss with profilometry increased with severity (no-EA>m-EA>s-EA, p < 0.001). D14 surface loss was higher than D7 for both methods except for the no-EA group with profilometry. CP-OCT and profilometry had moderate overall correlation (r = 0.70). Our results revealed that the currently proposed in vitro dental erosion-abrasion model is valid and could simulate lesions of different severities over time. CP-OCT was a suitable method for monitoring the EA lesions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100265&lng=en&tlng=enTooth WearDental EnamelTomography, Optical Coherence |
| spellingShingle | Maria Jacinta Rosario Romero Sávio José Cardoso Bezerra Daniel Fried Frank Lippert George Joseph Eckert Anderson Takeo Hara Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro Brazilian Oral Research Tooth Wear Dental Enamel Tomography, Optical Coherence |
| title | Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro |
| title_full | Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro |
| title_fullStr | Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro |
| title_full_unstemmed | Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro |
| title_short | Longitudinal assessment of dental erosion-abrasion by cross-polarization optical coherence tomography in vitro |
| title_sort | longitudinal assessment of dental erosion abrasion by cross polarization optical coherence tomography in vitro |
| topic | Tooth Wear Dental Enamel Tomography, Optical Coherence |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242023000100265&lng=en&tlng=en |
| work_keys_str_mv | AT mariajacintarosarioromero longitudinalassessmentofdentalerosionabrasionbycrosspolarizationopticalcoherencetomographyinvitro AT saviojosecardosobezerra longitudinalassessmentofdentalerosionabrasionbycrosspolarizationopticalcoherencetomographyinvitro AT danielfried longitudinalassessmentofdentalerosionabrasionbycrosspolarizationopticalcoherencetomographyinvitro AT franklippert longitudinalassessmentofdentalerosionabrasionbycrosspolarizationopticalcoherencetomographyinvitro AT georgejosepheckert longitudinalassessmentofdentalerosionabrasionbycrosspolarizationopticalcoherencetomographyinvitro AT andersontakeohara longitudinalassessmentofdentalerosionabrasionbycrosspolarizationopticalcoherencetomographyinvitro |