Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway
Huaier, as known as Trametes robiniophila Murr, is a traditional Chinese medicine. Various studies have demonstrated that Huaier could inhibit cancer progression and improve the prognosis of patients. In the present study, we comprehensively screened the expression profiles of lncRNAs, miRNAs, and m...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.01195/full |
| _version_ | 1818206279841087488 |
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| author | Wei Wang Wei Wang Xiaolong Wang Chen Li Tong Chen Ning Zhang Yiran Liang Yaming Li Hanwen Zhang Ying Liu Xiaojin Song Wenjing Zhao Bing Chen Lijuan Wang Qifeng Yang Qifeng Yang |
| author_facet | Wei Wang Wei Wang Xiaolong Wang Chen Li Tong Chen Ning Zhang Yiran Liang Yaming Li Hanwen Zhang Ying Liu Xiaojin Song Wenjing Zhao Bing Chen Lijuan Wang Qifeng Yang Qifeng Yang |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Huaier, as known as Trametes robiniophila Murr, is a traditional Chinese medicine. Various studies have demonstrated that Huaier could inhibit cancer progression and improve the prognosis of patients. In the present study, we comprehensively screened the expression profiles of lncRNAs, miRNAs, and mRNAs in Huaier-treated breast cancer cells. Using bioinformatic analysis, hub genes were identified and functionally annotated. Weighted gene coexpression network analysis was applied to construct the molecular network influenced by Huaier. Linc00339 was then found to play a critical role in Huaier-mediated cancer suppression. To validate the effects of linc00339 and identify the downstream targets, we performed in vitro and in vivo experiments. Finally, we identified that Huaier could inhibit the proliferation of breast cancer cells through modulating linc00339/miR-4656/CSNK2B signaling pathway. |
| first_indexed | 2024-12-12T04:10:30Z |
| format | Article |
| id | doaj.art-8f1dbd8173e249edb3216caf74f11d61 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T04:10:30Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-8f1dbd8173e249edb3216caf74f11d612022-12-22T00:38:37ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-11-01910.3389/fonc.2019.01195484230Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling PathwayWei Wang0Wei Wang1Xiaolong Wang2Chen Li3Tong Chen4Ning Zhang5Yiran Liang6Yaming Li7Hanwen Zhang8Ying Liu9Xiaojin Song10Wenjing Zhao11Bing Chen12Lijuan Wang13Qifeng Yang14Qifeng Yang15Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Thyroid and Breast Surgery, Jining No. 1 People's Hospital, Jining, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Pathology Tissue Bank, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Pathology Tissue Bank, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Pathology Tissue Bank, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Breast Surgery, Qilu Hospital, Shandong University, Jinan, ChinaDepartment of Pathology Tissue Bank, Qilu Hospital, Shandong University, Jinan, ChinaHuaier, as known as Trametes robiniophila Murr, is a traditional Chinese medicine. Various studies have demonstrated that Huaier could inhibit cancer progression and improve the prognosis of patients. In the present study, we comprehensively screened the expression profiles of lncRNAs, miRNAs, and mRNAs in Huaier-treated breast cancer cells. Using bioinformatic analysis, hub genes were identified and functionally annotated. Weighted gene coexpression network analysis was applied to construct the molecular network influenced by Huaier. Linc00339 was then found to play a critical role in Huaier-mediated cancer suppression. To validate the effects of linc00339 and identify the downstream targets, we performed in vitro and in vivo experiments. Finally, we identified that Huaier could inhibit the proliferation of breast cancer cells through modulating linc00339/miR-4656/CSNK2B signaling pathway.https://www.frontiersin.org/article/10.3389/fonc.2019.01195/fullHuaierbreast cancerbioinformaticslong non-coding RNAmicroarray |
| spellingShingle | Wei Wang Wei Wang Xiaolong Wang Chen Li Tong Chen Ning Zhang Yiran Liang Yaming Li Hanwen Zhang Ying Liu Xiaojin Song Wenjing Zhao Bing Chen Lijuan Wang Qifeng Yang Qifeng Yang Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway Frontiers in Oncology Huaier breast cancer bioinformatics long non-coding RNA microarray |
| title | Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway |
| title_full | Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway |
| title_fullStr | Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway |
| title_full_unstemmed | Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway |
| title_short | Huaier Suppresses Breast Cancer Progression via linc00339/miR-4656/CSNK2B Signaling Pathway |
| title_sort | huaier suppresses breast cancer progression via linc00339 mir 4656 csnk2b signaling pathway |
| topic | Huaier breast cancer bioinformatics long non-coding RNA microarray |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.01195/full |
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