Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species
The genus <i>Corynebacterium</i> is frequently found in the female urinary microbiome (FUM). In-depth characterization of <i>Corynebacterium</i> at the species level has been barely exploited. During ongoing FUM research studies, eight strains (c8Ua_144<sup>T</sup>...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/388 |
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| author | Elisabete Alves Cappelli Magdalena Ksiezarek Jacqueline Wolf Meina Neumann-Schaal Teresa Gonçalves Ribeiro Luísa Peixe |
| author_facet | Elisabete Alves Cappelli Magdalena Ksiezarek Jacqueline Wolf Meina Neumann-Schaal Teresa Gonçalves Ribeiro Luísa Peixe |
| author_sort | Elisabete Alves Cappelli |
| collection | DOAJ |
| description | The genus <i>Corynebacterium</i> is frequently found in the female urinary microbiome (FUM). In-depth characterization of <i>Corynebacterium</i> at the species level has been barely exploited. During ongoing FUM research studies, eight strains (c8Ua_144<sup>T</sup>, c8Ua_172<sup>T</sup>, c8Ua_174<sup>T</sup>, c8Ua_181<sup>T</sup>, c9Ua_112<sup>T</sup>, c19Ua_109<sup>T</sup>, c19Ua_121<sup>T</sup>, and c21Ua_68<sup>T</sup>) isolated from urine samples of healthy women or diagnosed with overactive bladder could not be allocated to any valid <i>Corynebacterium</i> species. In this work, we aimed to characterize these strains based on a polyphasic approach. The strains were Gram stain positive, rod to coccoid shaped, nonmotile, catalase positive, and oxidase negative. Phylogenetic analysis based on 16S rRNA and <i>rpoB</i> gene sequences indicated that all strains belonged to the genus <i>Corynebacterium</i>. The average nucleotide identity and digital DNA–DNA hybridization values among the genomes of the above eight strains and closely related type strains of the <i>Corynebacterium</i> genus were <95 (74.1%–93.9%) and <70% (22.2%–56.5%), respectively. Mycolic acids were identified in all strains. MK-8(H2) and/or MK-9(H2) were identified as the major menaquinones. The polar lipids’ pattern mostly consisted of diphosphatidylglycerol, phosphatidylglycerol, and glycophospholipids. The major fatty acid was C<sub>18:1</sub><i>ω</i>9<i>c</i>. <i>Corynebacterium lehmanniae</i> (c8Ua_144<sup>T</sup> = DSM 113405<sup>T</sup> = CCP 74<sup>T</sup>), <i>Corynebacterium meitnerae</i> (c8Ua_172<sup>T</sup> = DSM 113406<sup>T</sup> = CCP 75<sup>T</sup>), <i>Corynebacterium evansiae</i> (c8Ua_174<sup>T</sup> = DSM 113407<sup>T</sup> = CCP 76<sup>T</sup>), <i>Corynebacterium curieae</i> (c8Ua_181<sup>T</sup> = DSM 113408<sup>T</sup> = CCP 77<sup>T</sup>), <i>Corynebacterium macclintockiae</i> (c9Ua_112<sup>T</sup> = DSM 113409<sup>T</sup> = CCP 78<sup>T</sup>), <i>Corynebacterium hesseae</i> (c19Ua_109<sup>T</sup> = DSM 113410<sup>T</sup>= CCP 79<sup>T</sup>), <i>Corynebacterium marquesiae</i> (c19Ua_121<sup>T</sup> = DSM 113411<sup>T</sup> = CCP 80<sup>T</sup>), and <i>Corynebacterium yonathiae</i> (c21Ua_68<sup>T</sup> = DSM 113412<sup>T</sup> = CCP 81<sup>T</sup>) are proposed. This study evidenced that commonly used methodologies on FUM research presented limited resolution for discriminating <i>Corynebacterium</i> at the species level. Future research studying the biological mechanisms of the new <i>Corynebacterium</i> species here described may shed light on their possible beneficial role for healthy FUM. |
| first_indexed | 2024-03-11T08:23:16Z |
| format | Article |
| id | doaj.art-8f22a09eebda478197ce303cb2bad705 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T08:23:16Z |
| publishDate | 2023-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-8f22a09eebda478197ce303cb2bad7052023-11-16T22:15:10ZengMDPI AGMicroorganisms2076-26072023-02-0111238810.3390/microorganisms11020388Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> SpeciesElisabete Alves Cappelli0Magdalena Ksiezarek1Jacqueline Wolf2Meina Neumann-Schaal3Teresa Gonçalves Ribeiro4Luísa Peixe5Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLeibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, GermanyLeibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, GermanyAssociate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalThe genus <i>Corynebacterium</i> is frequently found in the female urinary microbiome (FUM). In-depth characterization of <i>Corynebacterium</i> at the species level has been barely exploited. During ongoing FUM research studies, eight strains (c8Ua_144<sup>T</sup>, c8Ua_172<sup>T</sup>, c8Ua_174<sup>T</sup>, c8Ua_181<sup>T</sup>, c9Ua_112<sup>T</sup>, c19Ua_109<sup>T</sup>, c19Ua_121<sup>T</sup>, and c21Ua_68<sup>T</sup>) isolated from urine samples of healthy women or diagnosed with overactive bladder could not be allocated to any valid <i>Corynebacterium</i> species. In this work, we aimed to characterize these strains based on a polyphasic approach. The strains were Gram stain positive, rod to coccoid shaped, nonmotile, catalase positive, and oxidase negative. Phylogenetic analysis based on 16S rRNA and <i>rpoB</i> gene sequences indicated that all strains belonged to the genus <i>Corynebacterium</i>. The average nucleotide identity and digital DNA–DNA hybridization values among the genomes of the above eight strains and closely related type strains of the <i>Corynebacterium</i> genus were <95 (74.1%–93.9%) and <70% (22.2%–56.5%), respectively. Mycolic acids were identified in all strains. MK-8(H2) and/or MK-9(H2) were identified as the major menaquinones. The polar lipids’ pattern mostly consisted of diphosphatidylglycerol, phosphatidylglycerol, and glycophospholipids. The major fatty acid was C<sub>18:1</sub><i>ω</i>9<i>c</i>. <i>Corynebacterium lehmanniae</i> (c8Ua_144<sup>T</sup> = DSM 113405<sup>T</sup> = CCP 74<sup>T</sup>), <i>Corynebacterium meitnerae</i> (c8Ua_172<sup>T</sup> = DSM 113406<sup>T</sup> = CCP 75<sup>T</sup>), <i>Corynebacterium evansiae</i> (c8Ua_174<sup>T</sup> = DSM 113407<sup>T</sup> = CCP 76<sup>T</sup>), <i>Corynebacterium curieae</i> (c8Ua_181<sup>T</sup> = DSM 113408<sup>T</sup> = CCP 77<sup>T</sup>), <i>Corynebacterium macclintockiae</i> (c9Ua_112<sup>T</sup> = DSM 113409<sup>T</sup> = CCP 78<sup>T</sup>), <i>Corynebacterium hesseae</i> (c19Ua_109<sup>T</sup> = DSM 113410<sup>T</sup>= CCP 79<sup>T</sup>), <i>Corynebacterium marquesiae</i> (c19Ua_121<sup>T</sup> = DSM 113411<sup>T</sup> = CCP 80<sup>T</sup>), and <i>Corynebacterium yonathiae</i> (c21Ua_68<sup>T</sup> = DSM 113412<sup>T</sup> = CCP 81<sup>T</sup>) are proposed. This study evidenced that commonly used methodologies on FUM research presented limited resolution for discriminating <i>Corynebacterium</i> at the species level. Future research studying the biological mechanisms of the new <i>Corynebacterium</i> species here described may shed light on their possible beneficial role for healthy FUM.https://www.mdpi.com/2076-2607/11/2/388<i>Corynebacterium</i>genome16S rRNA gene<i>rpoB</i>MALDI-TOF MSfemale urinary microbiome |
| spellingShingle | Elisabete Alves Cappelli Magdalena Ksiezarek Jacqueline Wolf Meina Neumann-Schaal Teresa Gonçalves Ribeiro Luísa Peixe Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species Microorganisms <i>Corynebacterium</i> genome 16S rRNA gene <i>rpoB</i> MALDI-TOF MS female urinary microbiome |
| title | Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species |
| title_full | Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species |
| title_fullStr | Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species |
| title_full_unstemmed | Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species |
| title_short | Expanding the Bacterial Diversity of the Female Urinary Microbiome: Description of Eight New <i>Corynebacterium</i> Species |
| title_sort | expanding the bacterial diversity of the female urinary microbiome description of eight new i corynebacterium i species |
| topic | <i>Corynebacterium</i> genome 16S rRNA gene <i>rpoB</i> MALDI-TOF MS female urinary microbiome |
| url | https://www.mdpi.com/2076-2607/11/2/388 |
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