Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands
Abstract Background Corsica is a large French island in the Mediterranean Sea with high human and animal migration rates, especially near wetlands where these migrations are particularly frequent. Among the livestock populations, cattle and sheep are widely present all across the entire Mediterranea...
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.956 |
| _version_ | 1797670176379895808 |
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| author | Baptiste Defaye Sara Moutailler Sébastien Grech‐Angelini Clémence Galon Sandrine Ferrandi Vanina Pasqualini Yann Quilichini |
| author_facet | Baptiste Defaye Sara Moutailler Sébastien Grech‐Angelini Clémence Galon Sandrine Ferrandi Vanina Pasqualini Yann Quilichini |
| author_sort | Baptiste Defaye |
| collection | DOAJ |
| description | Abstract Background Corsica is a large French island in the Mediterranean Sea with high human and animal migration rates, especially near wetlands where these migrations are particularly frequent. Among the livestock populations, cattle and sheep are widely present all across the entire Mediterranean region. Trade can be responsible for the circulation of numerous pathogens and their vectors, thereby representing a health and economic threat for the livestock industry. Objectives The objective of our study was to investigate the presence of pathogens in cattle and sheep farms in the wetlands of Corsica using a high‐throughput screening technique. Methods In our study, blood samples and ticks were collected from cattle and sheep in 20 municipalities near Corsican wetlands to screen for the presence of various types of pathogens. The samples were processed using a high‐throughput screening technique based on real‐time microfluidic PCR: 45 pathogens were screened in 47 samples simultaneously. Results A total of 372 cattle and 74 sheep were sampled, and 444 ticks were collected from cattle. Out of the eight tick species detected, the main one was Rhipicephalus bursa (38.7% of the ticks collected). From cattle blood samples, one species and two genera were found: Anaplasma marginale, Trypanosoma sp. and Babesia sp. in respectively 61.5%, 58.3% and 12.2% of the cattle blood samples. From sheep blood samples, 74.3% were positive for Anaplasma sp, 2.7% for Anaplasma ovis and 1.4% for Anaplasma capra. This is the first report of A. ovis DNA in blood samples from sheep in Corsica. Out of 444 the tick samples, 114 were positive: 77.2% for Rickettsia aeschlimannii, 20.2% for Rickettsia sp., 3.5% for Babesia sp. and 1.8% for Anaplasma sp. Among them, 2.7% were co‐infected with R. aeschlimannii and Babesia sp. Conclusions Our results confirm the extent of possible circulation of different pathogens near Corsican wetlands, not only in ticks collected from livestock but also directly in cattle and sheep, with two (Trypanosoma sp. and Babesia sp.) being detected for the first time in cattle, one for the first time in sheep (A. ovis) and one for the first time in Corsica (A. capra) |
| first_indexed | 2024-03-11T20:55:55Z |
| format | Article |
| id | doaj.art-8f22d20ba75a40bbadad23eb8401c760 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-11T20:55:55Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-8f22d20ba75a40bbadad23eb8401c7602023-09-30T12:10:55ZengWileyVeterinary Medicine and Science2053-10952022-11-01862662267710.1002/vms3.956Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlandsBaptiste Defaye0Sara Moutailler1Sébastien Grech‐Angelini2Clémence Galon3Sandrine Ferrandi4Vanina Pasqualini5Yann Quilichini6UMR CNRS SPE 6134 – Université de Corse Pascal Paoli Corte FranceANSES INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale Maisons‐Alfort FranceGroupement Technique Vétérinaire de Corse Corte FranceANSES INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale Maisons‐Alfort FranceGroupement Technique Vétérinaire de Corse Corte FranceUMR CNRS SPE 6134 – Université de Corse Pascal Paoli Corte FranceUMR CNRS SPE 6134 – Université de Corse Pascal Paoli Corte FranceAbstract Background Corsica is a large French island in the Mediterranean Sea with high human and animal migration rates, especially near wetlands where these migrations are particularly frequent. Among the livestock populations, cattle and sheep are widely present all across the entire Mediterranean region. Trade can be responsible for the circulation of numerous pathogens and their vectors, thereby representing a health and economic threat for the livestock industry. Objectives The objective of our study was to investigate the presence of pathogens in cattle and sheep farms in the wetlands of Corsica using a high‐throughput screening technique. Methods In our study, blood samples and ticks were collected from cattle and sheep in 20 municipalities near Corsican wetlands to screen for the presence of various types of pathogens. The samples were processed using a high‐throughput screening technique based on real‐time microfluidic PCR: 45 pathogens were screened in 47 samples simultaneously. Results A total of 372 cattle and 74 sheep were sampled, and 444 ticks were collected from cattle. Out of the eight tick species detected, the main one was Rhipicephalus bursa (38.7% of the ticks collected). From cattle blood samples, one species and two genera were found: Anaplasma marginale, Trypanosoma sp. and Babesia sp. in respectively 61.5%, 58.3% and 12.2% of the cattle blood samples. From sheep blood samples, 74.3% were positive for Anaplasma sp, 2.7% for Anaplasma ovis and 1.4% for Anaplasma capra. This is the first report of A. ovis DNA in blood samples from sheep in Corsica. Out of 444 the tick samples, 114 were positive: 77.2% for Rickettsia aeschlimannii, 20.2% for Rickettsia sp., 3.5% for Babesia sp. and 1.8% for Anaplasma sp. Among them, 2.7% were co‐infected with R. aeschlimannii and Babesia sp. Conclusions Our results confirm the extent of possible circulation of different pathogens near Corsican wetlands, not only in ticks collected from livestock but also directly in cattle and sheep, with two (Trypanosoma sp. and Babesia sp.) being detected for the first time in cattle, one for the first time in sheep (A. ovis) and one for the first time in Corsica (A. capra)https://doi.org/10.1002/vms3.956AnaplasmaBabesiacattlehigh‐throughput screening techniqueRickettsiasheep |
| spellingShingle | Baptiste Defaye Sara Moutailler Sébastien Grech‐Angelini Clémence Galon Sandrine Ferrandi Vanina Pasqualini Yann Quilichini Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands Veterinary Medicine and Science Anaplasma Babesia cattle high‐throughput screening technique Rickettsia sheep |
| title | Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands |
| title_full | Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands |
| title_fullStr | Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands |
| title_full_unstemmed | Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands |
| title_short | Detecting zoonotic and non‐zoonotic pathogens in livestock and their ticks in Corsican wetlands |
| title_sort | detecting zoonotic and non zoonotic pathogens in livestock and their ticks in corsican wetlands |
| topic | Anaplasma Babesia cattle high‐throughput screening technique Rickettsia sheep |
| url | https://doi.org/10.1002/vms3.956 |
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