Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea
Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-08-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671114001866 |
| _version_ | 1818305331223068672 |
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| author | M. Lucila Scimone Kellie M. Kravarik Sylvain W. Lapan Peter W. Reddien |
| author_facet | M. Lucila Scimone Kellie M. Kravarik Sylvain W. Lapan Peter W. Reddien |
| author_sort | M. Lucila Scimone |
| collection | DOAJ |
| description | Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts. |
| first_indexed | 2024-12-13T06:24:53Z |
| format | Article |
| id | doaj.art-8f262dec8e8c49f4b7a3a72304825bb8 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-13T06:24:53Z |
| publishDate | 2014-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-8f262dec8e8c49f4b7a3a72304825bb82022-12-21T23:56:46ZengElsevierStem Cell Reports2213-67112014-08-013233935210.1016/j.stemcr.2014.06.001Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterraneaM. Lucila Scimone0Kellie M. Kravarik1Sylvain W. Lapan2Peter W. Reddien3Howard Hughes Medical Institute, MIT Biology, and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USAHoward Hughes Medical Institute, MIT Biology, and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USAHoward Hughes Medical Institute, MIT Biology, and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USAHoward Hughes Medical Institute, MIT Biology, and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USAPlanarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts.http://www.sciencedirect.com/science/article/pii/S2213671114001866 |
| spellingShingle | M. Lucila Scimone Kellie M. Kravarik Sylvain W. Lapan Peter W. Reddien Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea Stem Cell Reports |
| title | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_full | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_fullStr | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_full_unstemmed | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_short | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_sort | neoblast specialization in regeneration of the planarian schmidtea mediterranea |
| url | http://www.sciencedirect.com/science/article/pii/S2213671114001866 |
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