Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol
<i>Aquilaria crassna</i> (AC) is a beneficial plant widely used to alleviate various health ailments. Nevertheless, the neuroprotection, antiaging, and xenobiotic detoxification against high benzo[a]pyrene induction have not been investigated. This study aimed to investigate the effects...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/18/3985 |
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| author | Nattaporn Pattarachotanant Panthakarn Rangsinth Watis Warayanon George Pak-Heng Leung Siriporn Chuchawankul Anchalee Prasansuklab Tewin Tencomnao |
| author_facet | Nattaporn Pattarachotanant Panthakarn Rangsinth Watis Warayanon George Pak-Heng Leung Siriporn Chuchawankul Anchalee Prasansuklab Tewin Tencomnao |
| author_sort | Nattaporn Pattarachotanant |
| collection | DOAJ |
| description | <i>Aquilaria crassna</i> (AC) is a beneficial plant widely used to alleviate various health ailments. Nevertheless, the neuroprotection, antiaging, and xenobiotic detoxification against high benzo[a]pyrene induction have not been investigated. This study aimed to investigate the effects of ethanolic extract of AC leaves (ACEE) in vitro using SH-SY5Y cells and in vivo using <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Neuroprotective activities and cell cycle progression were studied using SH-SY5Y cells. Additionally, <i>C. elegans</i> was used to determine longevity, health span, and transcriptional analysis. Furthermore, ACEE possible active compounds were analyzed by gas chromatograph–mass spectrometry (GC-MS) analysis and the possible active compounds were evaluated using a molecular docking study. First, ACEE possessed neuroprotective effects by normalizing cell cycle progression via the regulation of AhR/CYP1A1/cyclin D1 pathway. Next, ACEE played a role in xenobiotic detoxification in high B[a]P-induced <i>C. elegans</i> by the amelioration of lifespan reduction, and body length and size decrease through the reduction in gene expression in hexokinase (hxk) and CYP35 pathway. Finally, phytochemicals of ACEE were identified and we uncovered that clionasterol was the possible active constituent in powerfully inhibiting both CYP1A1 and hexokinase II receptor. Essentially, ACEE was recognized as a potential alternative medicine to defend against high B[a]P effects on neurotoxicity and xenobiotic detoxification. |
| first_indexed | 2024-03-10T22:20:28Z |
| format | Article |
| id | doaj.art-8f26c8b723f3471b9a139b1e39bbd9a2 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T22:20:28Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-8f26c8b723f3471b9a139b1e39bbd9a22023-11-19T12:18:40ZengMDPI AGNutrients2072-66432023-09-011518398510.3390/nu15183985Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes ClionasterolNattaporn Pattarachotanant0Panthakarn Rangsinth1Watis Warayanon2George Pak-Heng Leung3Siriporn Chuchawankul4Anchalee Prasansuklab5Tewin Tencomnao6Natural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaNatural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, ChinaDepartment of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit, Chulalongkorn University, Bangkok 10330, Thailand<i>Aquilaria crassna</i> (AC) is a beneficial plant widely used to alleviate various health ailments. Nevertheless, the neuroprotection, antiaging, and xenobiotic detoxification against high benzo[a]pyrene induction have not been investigated. This study aimed to investigate the effects of ethanolic extract of AC leaves (ACEE) in vitro using SH-SY5Y cells and in vivo using <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Neuroprotective activities and cell cycle progression were studied using SH-SY5Y cells. Additionally, <i>C. elegans</i> was used to determine longevity, health span, and transcriptional analysis. Furthermore, ACEE possible active compounds were analyzed by gas chromatograph–mass spectrometry (GC-MS) analysis and the possible active compounds were evaluated using a molecular docking study. First, ACEE possessed neuroprotective effects by normalizing cell cycle progression via the regulation of AhR/CYP1A1/cyclin D1 pathway. Next, ACEE played a role in xenobiotic detoxification in high B[a]P-induced <i>C. elegans</i> by the amelioration of lifespan reduction, and body length and size decrease through the reduction in gene expression in hexokinase (hxk) and CYP35 pathway. Finally, phytochemicals of ACEE were identified and we uncovered that clionasterol was the possible active constituent in powerfully inhibiting both CYP1A1 and hexokinase II receptor. Essentially, ACEE was recognized as a potential alternative medicine to defend against high B[a]P effects on neurotoxicity and xenobiotic detoxification.https://www.mdpi.com/2072-6643/15/18/3985xenobioticaryl hydrocarbon receptorCYP1A1CYP35hexokinasecyclin D1 |
| spellingShingle | Nattaporn Pattarachotanant Panthakarn Rangsinth Watis Warayanon George Pak-Heng Leung Siriporn Chuchawankul Anchalee Prasansuklab Tewin Tencomnao Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol Nutrients xenobiotic aryl hydrocarbon receptor CYP1A1 CYP35 hexokinase cyclin D1 |
| title | Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol |
| title_full | Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol |
| title_fullStr | Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol |
| title_full_unstemmed | Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol |
| title_short | Protective Effect of <i>Aquilaria crassna</i> Leaf Extract against Benzo[a]pyrene-Induced Toxicity in Neuronal Cells and <i>Caenorhabditis elegans</i>: Possible Active Constituent Includes Clionasterol |
| title_sort | protective effect of i aquilaria crassna i leaf extract against benzo a pyrene induced toxicity in neuronal cells and i caenorhabditis elegans i possible active constituent includes clionasterol |
| topic | xenobiotic aryl hydrocarbon receptor CYP1A1 CYP35 hexokinase cyclin D1 |
| url | https://www.mdpi.com/2072-6643/15/18/3985 |
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