Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot
Pear ring rot caused by <i>Botryosphaeria dothidea</i> severely affects the quality and storage life of pear fruit. Plant endophytes are potential new sources of biocontrol agents due to their abilities to produce novel bioactive products. In this work, we focused on an endophytic strain...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/9/9/1049 |
| _version_ | 1797579823037546496 |
|---|---|
| author | Suling Yang Meifang Song Limei Qi Guijun Liu Sijing Zhou Yuchen Qiao Ping Wang Haike Gu Liqin Wu |
| author_facet | Suling Yang Meifang Song Limei Qi Guijun Liu Sijing Zhou Yuchen Qiao Ping Wang Haike Gu Liqin Wu |
| author_sort | Suling Yang |
| collection | DOAJ |
| description | Pear ring rot caused by <i>Botryosphaeria dothidea</i> severely affects the quality and storage life of pear fruit. Plant endophytes are potential new sources of biocontrol agents due to their abilities to produce novel bioactive products. In this work, we focused on an endophytic strain B1, which was isolated from roots of <i>Dendrobium huoshanense</i>. Notably, strain B1 exhibited strongly inhibitory effects against the growth of <i>Botryosphaeria dothidea</i> with an inhibition rate of 73.2% after 1 week of co-cultivation. Furthermore, it displayed a broad-spectrum inhibitory activity. Strain B1 also effectively delayed the onset of ring rot caused by <i>B. dothidea</i> in pear fruit and reduced the lesion diameter by 73.4%. Strain B1 was identified as <i>Bacillus velezensis</i> based on core-genome phylogeny. Genome mining with antiSMASH revealed 13 potential gene clusters involved in antimicrobial metabolites. Three main groups of lipopeptides (surfactin, iturin and fengycin) were identified with MALDI-TOF-MS, and the lipopeptides in the inhibition zone were greatly upregulated by <i>B. dothidea</i>; especially, fengycin isoforms were detected with higher abundance. These results proved that lipopeptides produced by strain B1 had significant antagonistic effect on <i>B. dothidea</i>. In conclusion, our results suggest that strain B1 has potential applications in the biocontrol of <i>B. dothidea</i> in postharvest fruit. |
| first_indexed | 2024-03-10T22:41:22Z |
| format | Article |
| id | doaj.art-8f2b0037a81e48109ea69ca29c3bf6f4 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T22:41:22Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-8f2b0037a81e48109ea69ca29c3bf6f42023-11-19T10:59:29ZengMDPI AGHorticulturae2311-75242023-09-0199104910.3390/horticulturae9091049Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring RotSuling Yang0Meifang Song1Limei Qi2Guijun Liu3Sijing Zhou4Yuchen Qiao5Ping Wang6Haike Gu7Liqin Wu8Institute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaSchool of Electronic Engineering, Beijing University of Posts and Telecommunications, Beijing 100876, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaPear ring rot caused by <i>Botryosphaeria dothidea</i> severely affects the quality and storage life of pear fruit. Plant endophytes are potential new sources of biocontrol agents due to their abilities to produce novel bioactive products. In this work, we focused on an endophytic strain B1, which was isolated from roots of <i>Dendrobium huoshanense</i>. Notably, strain B1 exhibited strongly inhibitory effects against the growth of <i>Botryosphaeria dothidea</i> with an inhibition rate of 73.2% after 1 week of co-cultivation. Furthermore, it displayed a broad-spectrum inhibitory activity. Strain B1 also effectively delayed the onset of ring rot caused by <i>B. dothidea</i> in pear fruit and reduced the lesion diameter by 73.4%. Strain B1 was identified as <i>Bacillus velezensis</i> based on core-genome phylogeny. Genome mining with antiSMASH revealed 13 potential gene clusters involved in antimicrobial metabolites. Three main groups of lipopeptides (surfactin, iturin and fengycin) were identified with MALDI-TOF-MS, and the lipopeptides in the inhibition zone were greatly upregulated by <i>B. dothidea</i>; especially, fengycin isoforms were detected with higher abundance. These results proved that lipopeptides produced by strain B1 had significant antagonistic effect on <i>B. dothidea</i>. In conclusion, our results suggest that strain B1 has potential applications in the biocontrol of <i>B. dothidea</i> in postharvest fruit.https://www.mdpi.com/2311-7524/9/9/1049<i>Botryosphaeria dothidea</i>pear ring rotantagonism<i>Bacillusvelezensis</i>MALDI-TOF-MS |
| spellingShingle | Suling Yang Meifang Song Limei Qi Guijun Liu Sijing Zhou Yuchen Qiao Ping Wang Haike Gu Liqin Wu Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot Horticulturae <i>Botryosphaeria dothidea</i> pear ring rot antagonism <i>Bacillusvelezensis</i> MALDI-TOF-MS |
| title | Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot |
| title_full | Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot |
| title_fullStr | Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot |
| title_full_unstemmed | Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot |
| title_short | Effectiveness of a Novel Endophyte <i>Bacillus velezensis</i> Strain B1 in the Biocontrol of Pear Postharvest Ring Rot |
| title_sort | effectiveness of a novel endophyte i bacillus velezensis i strain b1 in the biocontrol of pear postharvest ring rot |
| topic | <i>Botryosphaeria dothidea</i> pear ring rot antagonism <i>Bacillusvelezensis</i> MALDI-TOF-MS |
| url | https://www.mdpi.com/2311-7524/9/9/1049 |
| work_keys_str_mv | AT sulingyang effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT meifangsong effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT limeiqi effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT guijunliu effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT sijingzhou effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT yuchenqiao effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT pingwang effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT haikegu effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot AT liqinwu effectivenessofanovelendophyteibacillusvelezensisistrainb1inthebiocontrolofpearpostharvestringrot |