The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice
A black yeast, Aureobasidium pullulans, extracellularly produces β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. The β-glucan is known to be an immunomodulatory agent, and β-glucan enriched A. pullulans cultured fluid (AP-CF) is used in supplements to maintain human health. Concanavalin...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021013803 |
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| author | Zhidan Wang Kuai Ma Masayuki Fujino Kisato Kusano Shuang-Qin Yi Atsushi Iwai Xiao-Kang Li |
| author_facet | Zhidan Wang Kuai Ma Masayuki Fujino Kisato Kusano Shuang-Qin Yi Atsushi Iwai Xiao-Kang Li |
| author_sort | Zhidan Wang |
| collection | DOAJ |
| description | A black yeast, Aureobasidium pullulans, extracellularly produces β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. The β-glucan is known to be an immunomodulatory agent, and β-glucan enriched A. pullulans cultured fluid (AP-CF) is used in supplements to maintain human health. Concanavalin A (ConA) is a lectin, and when injected it is known to cause T cell mediated autoimmune hepatitis in mice. The present study investigated the effects of oral administration of AP-CF on ConA injection in mice. The results demonstrated that increases in serum alanine transaminase (ALT) levels after ConA injection were significantly suppressed in an AP-CF administered group of mice. To understand the mechanism of the ALT lowering effects of AP-CF, we used Foxp3 (forkhead box P3) knock-in mice which express the green fluorescent protein (GFP) in Foxp3 induced cells, and the effects of AP-CF on the regulatory T cell (Treg) populations were investigated. The results show that the basal level of Foxp3+ Treg populations in peripheral blood lymphocytes, liver infiltrating lymphocytes, and splenocytes was decreased after 7 days of administration of AP-CF. These findings suggest that oral administration of AP-CF suppresses the basal level of inflammation, and that it may be postulated to be involved in the ALT lowering effects of AP-CF. |
| first_indexed | 2024-12-14T17:53:18Z |
| format | Article |
| id | doaj.art-8f2b57768eff43b881c94736b1a46e85 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-14T17:53:18Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8f2b57768eff43b881c94736b1a46e852022-12-21T22:52:35ZengElsevierHeliyon2405-84402021-06-0176e07277The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected miceZhidan Wang0Kuai Ma1Masayuki Fujino2Kisato Kusano3Shuang-Qin Yi4Atsushi Iwai5Xiao-Kang Li6Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Laboratory of Functional Morphology, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, JapanDivision of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, JapanDivision of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, JapanAureo Co., Ltd., Chiba, JapanLaboratory of Functional Morphology, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, JapanAureo Co., Ltd., Chiba, Japan; Aureo Science Co., Ltd., Hokkaido, Japan; Corresponding author.Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Laboratory of Functional Morphology, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan; Corresponding author.A black yeast, Aureobasidium pullulans, extracellularly produces β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. The β-glucan is known to be an immunomodulatory agent, and β-glucan enriched A. pullulans cultured fluid (AP-CF) is used in supplements to maintain human health. Concanavalin A (ConA) is a lectin, and when injected it is known to cause T cell mediated autoimmune hepatitis in mice. The present study investigated the effects of oral administration of AP-CF on ConA injection in mice. The results demonstrated that increases in serum alanine transaminase (ALT) levels after ConA injection were significantly suppressed in an AP-CF administered group of mice. To understand the mechanism of the ALT lowering effects of AP-CF, we used Foxp3 (forkhead box P3) knock-in mice which express the green fluorescent protein (GFP) in Foxp3 induced cells, and the effects of AP-CF on the regulatory T cell (Treg) populations were investigated. The results show that the basal level of Foxp3+ Treg populations in peripheral blood lymphocytes, liver infiltrating lymphocytes, and splenocytes was decreased after 7 days of administration of AP-CF. These findings suggest that oral administration of AP-CF suppresses the basal level of inflammation, and that it may be postulated to be involved in the ALT lowering effects of AP-CF.http://www.sciencedirect.com/science/article/pii/S2405844021013803β-glucanAureobasidium pullulansConcanavalin ARegulatory T cellFoxp3 |
| spellingShingle | Zhidan Wang Kuai Ma Masayuki Fujino Kisato Kusano Shuang-Qin Yi Atsushi Iwai Xiao-Kang Li The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice Heliyon β-glucan Aureobasidium pullulans Concanavalin A Regulatory T cell Foxp3 |
| title | The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice |
| title_full | The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice |
| title_fullStr | The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice |
| title_full_unstemmed | The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice |
| title_short | The effects of oral administration of Aureobasidium pullulans-cultured fluid containing β-glucan on concanavalin A injected mice |
| title_sort | effects of oral administration of aureobasidium pullulans cultured fluid containing β glucan on concanavalin a injected mice |
| topic | β-glucan Aureobasidium pullulans Concanavalin A Regulatory T cell Foxp3 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021013803 |
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