Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus
The prevalence and virulence of pathogens such as methicillin-resistant Staphylococcus (S.) aureus (MRSA), which can cause recurrent skin infections, are of significant clinical concern. Prolonged antibiotic exposure to treat or decolonize S. aureus contributes to development of antibiotic resistanc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754240/?tool=EBI |
| _version_ | 1797979749748834304 |
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| author | Changhui Guan Peter J. Larson Elizabeth Fleming Alexander P. Tikhonov Sara Mootien Trudy H. Grossman Caroline Golino Julia Oh |
| author_facet | Changhui Guan Peter J. Larson Elizabeth Fleming Alexander P. Tikhonov Sara Mootien Trudy H. Grossman Caroline Golino Julia Oh |
| author_sort | Changhui Guan |
| collection | DOAJ |
| description | The prevalence and virulence of pathogens such as methicillin-resistant Staphylococcus (S.) aureus (MRSA), which can cause recurrent skin infections, are of significant clinical concern. Prolonged antibiotic exposure to treat or decolonize S. aureus contributes to development of antibiotic resistance, as well as depletion of the microbiome, and its numerous beneficial functions. We hypothesized an engineered skin probiotic with the ability to selectively deliver antimicrobials only in the presence of the target organism could provide local bioremediation of pathogen colonization. We constructed a biosensing S. epidermidis capable of detecting the presence of S. aureus quorum sensing autoinducer peptide and producing lysostaphin in response. Here, we demonstrate in vitro activity of this biosensor and present and discuss challenges to deployment of this and other engineered topical skin probiotics. |
| first_indexed | 2024-04-11T05:44:38Z |
| format | Article |
| id | doaj.art-8f3391a7129f4ed29e5396d5475f6287 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T05:44:38Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8f3391a7129f4ed29e5396d5475f62872022-12-22T04:42:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureusChanghui GuanPeter J. LarsonElizabeth FlemingAlexander P. TikhonovSara MootienTrudy H. GrossmanCaroline GolinoJulia OhThe prevalence and virulence of pathogens such as methicillin-resistant Staphylococcus (S.) aureus (MRSA), which can cause recurrent skin infections, are of significant clinical concern. Prolonged antibiotic exposure to treat or decolonize S. aureus contributes to development of antibiotic resistance, as well as depletion of the microbiome, and its numerous beneficial functions. We hypothesized an engineered skin probiotic with the ability to selectively deliver antimicrobials only in the presence of the target organism could provide local bioremediation of pathogen colonization. We constructed a biosensing S. epidermidis capable of detecting the presence of S. aureus quorum sensing autoinducer peptide and producing lysostaphin in response. Here, we demonstrate in vitro activity of this biosensor and present and discuss challenges to deployment of this and other engineered topical skin probiotics.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754240/?tool=EBI |
| spellingShingle | Changhui Guan Peter J. Larson Elizabeth Fleming Alexander P. Tikhonov Sara Mootien Trudy H. Grossman Caroline Golino Julia Oh Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus PLoS ONE |
| title | Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus |
| title_full | Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus |
| title_fullStr | Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus |
| title_short | Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus |
| title_sort | engineering a detect and destroy skin probiotic to combat methicillin resistant staphylococcus aureus |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754240/?tool=EBI |
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