A 3D Analysis of Cleared Human Melanoma
Cutaneous melanoma is one of the most aggressive and deadliest cancers in human beings due to its invasiveness and other factors. Histopathological analysis is crucial for a proper diagnosis. Optical tissue clearing is a novel field that allows 3D image acquisition of large-scale biological tissues....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/7/1580 |
| _version_ | 1797433829963595776 |
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| author | Vicente Llorente Daniel Sanderson Alejandro Martín-Gorgojo Rafael Samaniego Manuel Desco María Victoria Gómez-Gaviro |
| author_facet | Vicente Llorente Daniel Sanderson Alejandro Martín-Gorgojo Rafael Samaniego Manuel Desco María Victoria Gómez-Gaviro |
| author_sort | Vicente Llorente |
| collection | DOAJ |
| description | Cutaneous melanoma is one of the most aggressive and deadliest cancers in human beings due to its invasiveness and other factors. Histopathological analysis is crucial for a proper diagnosis. Optical tissue clearing is a novel field that allows 3D image acquisition of large-scale biological tissues. Optical clearing and immunolabeling for 3D fluorescence imaging has yet to be extensively applied to melanoma. In the present manuscript, we establish, for the first time, an optical clearing and immunostaining procedure for human melanoma and human cell line-derived melanoma xenograft models using the CUBIC (clear, unobstructed brain imaging cocktails) technique. We have successfully cleared the samples and achieved 3D volumetric visualization of the tumor microenvironment, vasculature, and cell populations. |
| first_indexed | 2024-03-09T10:22:23Z |
| format | Article |
| id | doaj.art-8f350c56eeb54ad2899f6517debbf3fe |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T10:22:23Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-8f350c56eeb54ad2899f6517debbf3fe2023-12-01T21:55:59ZengMDPI AGBiomedicines2227-90592022-07-01107158010.3390/biomedicines10071580A 3D Analysis of Cleared Human MelanomaVicente Llorente0Daniel Sanderson1Alejandro Martín-Gorgojo2Rafael Samaniego3Manuel Desco4María Victoria Gómez-Gaviro5Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Doctor Esquerdo 46, 28007 Madrid, SpainInstituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Doctor Esquerdo 46, 28007 Madrid, SpainInstituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Doctor Esquerdo 46, 28007 Madrid, SpainInstituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Doctor Esquerdo 46, 28007 Madrid, SpainInstituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Doctor Esquerdo 46, 28007 Madrid, SpainInstituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Doctor Esquerdo 46, 28007 Madrid, SpainCutaneous melanoma is one of the most aggressive and deadliest cancers in human beings due to its invasiveness and other factors. Histopathological analysis is crucial for a proper diagnosis. Optical tissue clearing is a novel field that allows 3D image acquisition of large-scale biological tissues. Optical clearing and immunolabeling for 3D fluorescence imaging has yet to be extensively applied to melanoma. In the present manuscript, we establish, for the first time, an optical clearing and immunostaining procedure for human melanoma and human cell line-derived melanoma xenograft models using the CUBIC (clear, unobstructed brain imaging cocktails) technique. We have successfully cleared the samples and achieved 3D volumetric visualization of the tumor microenvironment, vasculature, and cell populations.https://www.mdpi.com/2227-9059/10/7/1580melanomavasculature3D imagingtissue clearingSPIMlight-sheet microscopy |
| spellingShingle | Vicente Llorente Daniel Sanderson Alejandro Martín-Gorgojo Rafael Samaniego Manuel Desco María Victoria Gómez-Gaviro A 3D Analysis of Cleared Human Melanoma Biomedicines melanoma vasculature 3D imaging tissue clearing SPIM light-sheet microscopy |
| title | A 3D Analysis of Cleared Human Melanoma |
| title_full | A 3D Analysis of Cleared Human Melanoma |
| title_fullStr | A 3D Analysis of Cleared Human Melanoma |
| title_full_unstemmed | A 3D Analysis of Cleared Human Melanoma |
| title_short | A 3D Analysis of Cleared Human Melanoma |
| title_sort | 3d analysis of cleared human melanoma |
| topic | melanoma vasculature 3D imaging tissue clearing SPIM light-sheet microscopy |
| url | https://www.mdpi.com/2227-9059/10/7/1580 |
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