Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria
This study focused on antibiotic resistance genes as emerging contaminants with potential global human health implications. Intensive livestock farming has been identified as a major contributor to the spread of resistant bacteria and genes. The study examined antimicrobial-resistant Escherichia col...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-09-01
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| Series: | Folia Veterinaria |
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| Online Access: | https://doi.org/10.2478/fv-2023-0024 |
| _version_ | 1797660988949921792 |
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| author | Yusuf Muhammad S. Kabir Junaidu Bello Mohammed Aliyu Mohammed B. Esonu Daniel O. Yusuf Fatima L. Babashani Mohammed |
| author_facet | Yusuf Muhammad S. Kabir Junaidu Bello Mohammed Aliyu Mohammed B. Esonu Daniel O. Yusuf Fatima L. Babashani Mohammed |
| author_sort | Yusuf Muhammad S. |
| collection | DOAJ |
| description | This study focused on antibiotic resistance genes as emerging contaminants with potential global human health implications. Intensive livestock farming has been identified as a major contributor to the spread of resistant bacteria and genes. The study examined antimicrobial-resistant Escherichia coli and tetracycline-resistant genes in raw milk from commercial dairy farms in Kano State. Out of 300 registered farms, 54 (18 %) were purposively sampled for the study. A total of 313 milk samples were collected and processed through enrichment and inoculation on selective media for Escherichia coli isolation. The antibiogram pattern of the isolated Escherichia coli strains was assessed using the disk diffusion method. The results revealed resistance to various antimicrobial agents, with no resistance to quinolones but high resistance to ampicillin (100 %), erythromycin (73.3 %), and tetracycline (46.7 %), among others. The multiplex polymer-ase chain reaction was conducted on all Escherichia coli isolates to detect tet genes (tet A, B, C, D, and M), and one isolate carried the tet M resistance gene, while six (40 %) others carried the tet A resistance gene. The study concludes that a significant proportion of the cultured Escherichia coli strains were resistant to one or more tested antibiotics, indicating a potential public health threat associated with Escherichia coli contamination in raw milk. We recommend implementing robust regulatory policies governing the use and sales of antimicrobials in animal production. Furthermore, we suggest further investigation into other resistant genes that these isolates might carry to better understand the extent of antibiotic resistance in the region. |
| first_indexed | 2024-03-11T18:37:59Z |
| format | Article |
| id | doaj.art-8f366d1592cf42e292dcb943bbe85bca |
| institution | Directory Open Access Journal |
| issn | 2453-7837 |
| language | English |
| last_indexed | 2024-03-11T18:37:59Z |
| publishDate | 2023-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Veterinaria |
| spelling | doaj.art-8f366d1592cf42e292dcb943bbe85bca2023-10-12T14:08:28ZengSciendoFolia Veterinaria2453-78372023-09-01673253210.2478/fv-2023-0024Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in NigeriaYusuf Muhammad S.0Kabir Junaidu1Bello Mohammed2Aliyu Mohammed B.3Esonu Daniel O.4Yusuf Fatima L.5Babashani Mohammed6Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, ZariaDepartment of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, ZariaDepartment of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, ZariaDepartment of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, ZariaDepartment of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, ZariaDepartment of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, ZariaVeterinary Teaching Hospital, Ahmadu Bello University, ZariaNigeriaThis study focused on antibiotic resistance genes as emerging contaminants with potential global human health implications. Intensive livestock farming has been identified as a major contributor to the spread of resistant bacteria and genes. The study examined antimicrobial-resistant Escherichia coli and tetracycline-resistant genes in raw milk from commercial dairy farms in Kano State. Out of 300 registered farms, 54 (18 %) were purposively sampled for the study. A total of 313 milk samples were collected and processed through enrichment and inoculation on selective media for Escherichia coli isolation. The antibiogram pattern of the isolated Escherichia coli strains was assessed using the disk diffusion method. The results revealed resistance to various antimicrobial agents, with no resistance to quinolones but high resistance to ampicillin (100 %), erythromycin (73.3 %), and tetracycline (46.7 %), among others. The multiplex polymer-ase chain reaction was conducted on all Escherichia coli isolates to detect tet genes (tet A, B, C, D, and M), and one isolate carried the tet M resistance gene, while six (40 %) others carried the tet A resistance gene. The study concludes that a significant proportion of the cultured Escherichia coli strains were resistant to one or more tested antibiotics, indicating a potential public health threat associated with Escherichia coli contamination in raw milk. We recommend implementing robust regulatory policies governing the use and sales of antimicrobials in animal production. Furthermore, we suggest further investigation into other resistant genes that these isolates might carry to better understand the extent of antibiotic resistance in the region.https://doi.org/10.2478/fv-2023-0024antimicrobial resistancedairy cowsescherichia coliraw milk |
| spellingShingle | Yusuf Muhammad S. Kabir Junaidu Bello Mohammed Aliyu Mohammed B. Esonu Daniel O. Yusuf Fatima L. Babashani Mohammed Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria Folia Veterinaria antimicrobial resistance dairy cows escherichia coli raw milk |
| title | Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria |
| title_full | Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria |
| title_fullStr | Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria |
| title_full_unstemmed | Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria |
| title_short | Occurrence of Tet Gene-Bearing Antimicrobial-Resistant Escherichia coli From Dairy Farms in Nigeria |
| title_sort | occurrence of tet gene bearing antimicrobial resistant escherichia coli from dairy farms in nigeria |
| topic | antimicrobial resistance dairy cows escherichia coli raw milk |
| url | https://doi.org/10.2478/fv-2023-0024 |
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