Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children
Background. Russian Federation is included in the list of 30 countries with the highest burden of tuberculosis, including MDR tuberculosis. The most important part of this problem is the primary MDR/XDR TB in children.The aim: a comparative analysis of the phenotypic and genotypic profile of drug re...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2022-12-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/3884 |
| _version_ | 1826853155261906944 |
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| author | P. A. Khromova S. N. Zhdanova N. S. Solovieva V. V. Sinkov A. E. Masharsky A. A. Vyazovaya I. V. Mokrousov L. V. Rychkova L. I. Kolesnikova V. Yu. Zhuravlev O. B. Ogarkov |
| author_facet | P. A. Khromova S. N. Zhdanova N. S. Solovieva V. V. Sinkov A. E. Masharsky A. A. Vyazovaya I. V. Mokrousov L. V. Rychkova L. I. Kolesnikova V. Yu. Zhuravlev O. B. Ogarkov |
| author_sort | P. A. Khromova |
| collection | DOAJ |
| description | Background. Russian Federation is included in the list of 30 countries with the highest burden of tuberculosis, including MDR tuberculosis. The most important part of this problem is the primary MDR/XDR TB in children.The aim: a comparative analysis of the phenotypic and genotypic profile of drug resistance to anti-tuberculosis drugs (ATP) according to whole genome sequencing of M. tuberculosis strains from children.Materials and methods. Whole genome sequencing (WGS) results of 61 M. tuberculosis isolates from children with tuberculosis in 2006–2020 in the Russian Federation were analyzed for anti-TB drug resistance mutations, according to the WHO catalog and were compared with the results of phenotypic drug sensitivity.Results. The M. tuberculosis belonged to two genetic groups: Beijing genotype – 82 % (50/61) dominant Central Asian Russian (31/50) and B0/W148 (16/50) subtypes, and non-Beijing (Ural, S, LAM) – 18 % (11/61). Three isolates belonged to Asian Ancestral subtype (3/50). Of the 61 isolates, only 14.7 % (9/61) were sensitive to antiTB drugs, 49.2 % (30/61) were MDR and 14.7 % (9/61) were pre-XDR. Comparison of the resistance profile (MDR/pre-XDR) with genotype revealed an upward shift for Beijing isolates, in particular Beijing B0/W148 (15/16) subline compared to other Beijing (19/34) (Chi-square with Yates correction = 5.535; p < 0.05) and nonBeijing (5/12) (Chi-square with Yates correction = 6.741; p < 0.05) subtypes. Discrepancies between genotypic and phenotypic drug resistance profiles were found in 11.5 % (7/61) of cases.Conclusions. Based on the analysis of WGS data, the genotypic characteristics of M. tuberculosis and the most complete set of drug resistance mutations were obtained, indicating a significant prevalence in MDR and pre-XDR TB of cases caused by epidemic subtypes of Beijing (B0/W148 and Central Asian Russian). The molecular mechanisms of adaptation of M. tuberculosis to the treatment of anti-TB drugs are not unique for the child population but reflect the general processes of the spread of MDR/XDR in Russia. |
| first_indexed | 2024-03-08T12:15:05Z |
| format | Article |
| id | doaj.art-8f3a7066cd6f4d0dbdd968b8bbc730bb |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2025-02-16T17:22:00Z |
| publishDate | 2022-12-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-8f3a7066cd6f4d0dbdd968b8bbc730bb2025-01-27T16:49:01ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962022-12-0176829110.29413/ABS.2022-7.6.82473Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB childrenP. A. Khromova0S. N. Zhdanova1N. S. Solovieva2V. V. Sinkov3A. E. Masharsky4A. A. Vyazovaya5I. V. Mokrousov6L. V. Rychkova7L. I. Kolesnikova8V. Yu. Zhuravlev9O. B. Ogarkov10Scientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsSaint Petersburg Research Institute of PhthisiopulmonologyScientific Centre for Family Health and Human Reproduction ProblemsThe Bio-Bank Resource Center, Research Park, Saint Petersburg State UniversitySaint Petersburg Pasteur InstituteSaint Petersburg Pasteur InstituteScientific Centre for Family Health and Human Reproduction ProblemsScientific Centre for Family Health and Human Reproduction ProblemsSaint Petersburg Research Institute of PhthisiopulmonologyScientific Centre for Family Health and Human Reproduction ProblemsBackground. Russian Federation is included in the list of 30 countries with the highest burden of tuberculosis, including MDR tuberculosis. The most important part of this problem is the primary MDR/XDR TB in children.The aim: a comparative analysis of the phenotypic and genotypic profile of drug resistance to anti-tuberculosis drugs (ATP) according to whole genome sequencing of M. tuberculosis strains from children.Materials and methods. Whole genome sequencing (WGS) results of 61 M. tuberculosis isolates from children with tuberculosis in 2006–2020 in the Russian Federation were analyzed for anti-TB drug resistance mutations, according to the WHO catalog and were compared with the results of phenotypic drug sensitivity.Results. The M. tuberculosis belonged to two genetic groups: Beijing genotype – 82 % (50/61) dominant Central Asian Russian (31/50) and B0/W148 (16/50) subtypes, and non-Beijing (Ural, S, LAM) – 18 % (11/61). Three isolates belonged to Asian Ancestral subtype (3/50). Of the 61 isolates, only 14.7 % (9/61) were sensitive to antiTB drugs, 49.2 % (30/61) were MDR and 14.7 % (9/61) were pre-XDR. Comparison of the resistance profile (MDR/pre-XDR) with genotype revealed an upward shift for Beijing isolates, in particular Beijing B0/W148 (15/16) subline compared to other Beijing (19/34) (Chi-square with Yates correction = 5.535; p < 0.05) and nonBeijing (5/12) (Chi-square with Yates correction = 6.741; p < 0.05) subtypes. Discrepancies between genotypic and phenotypic drug resistance profiles were found in 11.5 % (7/61) of cases.Conclusions. Based on the analysis of WGS data, the genotypic characteristics of M. tuberculosis and the most complete set of drug resistance mutations were obtained, indicating a significant prevalence in MDR and pre-XDR TB of cases caused by epidemic subtypes of Beijing (B0/W148 and Central Asian Russian). The molecular mechanisms of adaptation of M. tuberculosis to the treatment of anti-TB drugs are not unique for the child population but reflect the general processes of the spread of MDR/XDR in Russia.https://www.actabiomedica.ru/jour/article/view/3884mycobacterium tuberculosiswhole genome sequencingchildrenmultidrug resistancemutation spectrumgenotype |
| spellingShingle | P. A. Khromova S. N. Zhdanova N. S. Solovieva V. V. Sinkov A. E. Masharsky A. A. Vyazovaya I. V. Mokrousov L. V. Rychkova L. I. Kolesnikova V. Yu. Zhuravlev O. B. Ogarkov Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children Acta Biomedica Scientifica mycobacterium tuberculosis whole genome sequencing children multidrug resistance mutation spectrum genotype |
| title | Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children |
| title_full | Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children |
| title_fullStr | Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children |
| title_full_unstemmed | Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children |
| title_short | Genotypic and phenotypic characteristics of <i>Mycobacterium tuberculosis</i> drug resistance in TB children |
| title_sort | genotypic and phenotypic characteristics of i mycobacterium tuberculosis i drug resistance in tb children |
| topic | mycobacterium tuberculosis whole genome sequencing children multidrug resistance mutation spectrum genotype |
| url | https://www.actabiomedica.ru/jour/article/view/3884 |
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