Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy
Abstract Background Integrating immunohistochemistry (IHC) and clonality testing with histopathology may improve the ability to differentiate inflammatory bowel disease (IBD) and alimentary small cell lymphoma (LSA) in cats. Hypothesis/Objectives To evaluate the utility of histopathology, IHC, and c...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16000 |
| _version_ | 1798030131846971392 |
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| author | Betty Chow Steve L. Hill Keith P. Richter Sina Marsilio Mark R. Ackermann Jonathan A. Lidbury Jan S. Suchodolski Sarah Cocker Jörg M. Steiner |
| author_facet | Betty Chow Steve L. Hill Keith P. Richter Sina Marsilio Mark R. Ackermann Jonathan A. Lidbury Jan S. Suchodolski Sarah Cocker Jörg M. Steiner |
| author_sort | Betty Chow |
| collection | DOAJ |
| description | Abstract Background Integrating immunohistochemistry (IHC) and clonality testing with histopathology may improve the ability to differentiate inflammatory bowel disease (IBD) and alimentary small cell lymphoma (LSA) in cats. Hypothesis/Objectives To evaluate the utility of histopathology, IHC, and clonality testing to differentiate between IBD and LSA and agreement of diagnostic results for endoscopic biopsy (EB) samples from the upper (USI) and lower small intestine (LSI). Animals Fifty‐seven cats with IBD or LSA. Methods All cases were categorized as definitive IBD (DefIBD), possible LSA (PossLSA), probable LSA (ProbLSA), or definitive LSA (DefLSA) based on histopathology alone. Results from IHC and clonality testing were integrated. Results Based on histopathology alone, 24/57 (42.1%), 15/57 (26.3%), and 18/57 (31.6%) cats were diagnosed with DefIBD, PossLSA or ProbLSA, and DefLSA, respectively. After integrating IHC and clonality testing, 11/24 cases (45.8%) and 15/15 cases (100%) previously categorized as DefIBD and PossLSA or ProbLSA, respectively, were reclassified as LSA. A final diagnosis of IBD and LSA was reported in 13/57 (22.8%) and 44/57 (77.2%) cats, respectively. Agreement between USI and LSI samples was moderate based on histopathology alone (κ = 0.66) and after integrating IHC and clonality testing (κ = 0.70). However, only 1/44 (2.3%) of the LSA cases was diagnosed based on LSI biopsy alone. Conclusions and Clinical Importance Integrating IHC and clonality testing increased the number of cases diagnosed with LSA, but the consequence for patient outcome is unclear. There was moderate agreement between USI and LSI samples. Samples from the LSI rarely changed the diagnosis. |
| first_indexed | 2024-04-11T19:36:22Z |
| format | Article |
| id | doaj.art-8f3b3c8653774bd2b2782cf5383c3c41 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-11T19:36:22Z |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-8f3b3c8653774bd2b2782cf5383c3c412022-12-22T04:06:51ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762021-01-0135119019810.1111/jvim.16000Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathyBetty Chow0Steve L. Hill1Keith P. Richter2Sina Marsilio3Mark R. Ackermann4Jonathan A. Lidbury5Jan S. Suchodolski6Sarah Cocker7Jörg M. Steiner8Veterinary Specialty Hospital by Ethos Veterinary Health San Diego California USAVeterinary Specialty Hospital by Ethos Veterinary Health San Diego California USAVeterinary Specialty Hospital by Ethos Veterinary Health San Diego California USADepartment of Medicine and Epidemiology University of California, School of Veterinary Medicine Davis California USADepartment of Biomedical Sciences and Oregon Veterinary Diagnostic Laboratory, Carlson College of Veterinary Medicine Oregon State University Corvallis Oregon USAGastrointestinal Laboratory Texas A&M University College Station Texas USAGastrointestinal Laboratory Texas A&M University College Station Texas USAVeterinary Specialty Hospital by Ethos Veterinary Health San Diego California USAGastrointestinal Laboratory Texas A&M University College Station Texas USAAbstract Background Integrating immunohistochemistry (IHC) and clonality testing with histopathology may improve the ability to differentiate inflammatory bowel disease (IBD) and alimentary small cell lymphoma (LSA) in cats. Hypothesis/Objectives To evaluate the utility of histopathology, IHC, and clonality testing to differentiate between IBD and LSA and agreement of diagnostic results for endoscopic biopsy (EB) samples from the upper (USI) and lower small intestine (LSI). Animals Fifty‐seven cats with IBD or LSA. Methods All cases were categorized as definitive IBD (DefIBD), possible LSA (PossLSA), probable LSA (ProbLSA), or definitive LSA (DefLSA) based on histopathology alone. Results from IHC and clonality testing were integrated. Results Based on histopathology alone, 24/57 (42.1%), 15/57 (26.3%), and 18/57 (31.6%) cats were diagnosed with DefIBD, PossLSA or ProbLSA, and DefLSA, respectively. After integrating IHC and clonality testing, 11/24 cases (45.8%) and 15/15 cases (100%) previously categorized as DefIBD and PossLSA or ProbLSA, respectively, were reclassified as LSA. A final diagnosis of IBD and LSA was reported in 13/57 (22.8%) and 44/57 (77.2%) cats, respectively. Agreement between USI and LSI samples was moderate based on histopathology alone (κ = 0.66) and after integrating IHC and clonality testing (κ = 0.70). However, only 1/44 (2.3%) of the LSA cases was diagnosed based on LSI biopsy alone. Conclusions and Clinical Importance Integrating IHC and clonality testing increased the number of cases diagnosed with LSA, but the consequence for patient outcome is unclear. There was moderate agreement between USI and LSI samples. Samples from the LSI rarely changed the diagnosis.https://doi.org/10.1111/jvim.16000clonalityfeline chronic enteropathyimmunohistochemistryinflammatory bowel diseasePARRsmall cell lymphoma |
| spellingShingle | Betty Chow Steve L. Hill Keith P. Richter Sina Marsilio Mark R. Ackermann Jonathan A. Lidbury Jan S. Suchodolski Sarah Cocker Jörg M. Steiner Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy Journal of Veterinary Internal Medicine clonality feline chronic enteropathy immunohistochemistry inflammatory bowel disease PARR small cell lymphoma |
| title | Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy |
| title_full | Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy |
| title_fullStr | Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy |
| title_full_unstemmed | Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy |
| title_short | Comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy |
| title_sort | comprehensive comparison of upper and lower endoscopic small intestinal biopsy in cats with chronic enteropathy |
| topic | clonality feline chronic enteropathy immunohistochemistry inflammatory bowel disease PARR small cell lymphoma |
| url | https://doi.org/10.1111/jvim.16000 |
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