Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i>
Leaves and siliques are important organs associated with dry matter biosynthesis and vegetable oil accumulation in plants. We identified and characterized a novel locus controlling leaf and silique development using the <i>Brassica napus</i> mutant <i>Bnud1</i>, which has dow...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/4/3069 |
| Summary: | Leaves and siliques are important organs associated with dry matter biosynthesis and vegetable oil accumulation in plants. We identified and characterized a novel locus controlling leaf and silique development using the <i>Brassica napus</i> mutant <i>Bnud1</i>, which has downward-pointing siliques and up-curling leaves. The inheritance analysis showed that the up-curling leaf and downward-pointing silique traits are controlled by one dominant locus (<i>BnUD1</i>) in populations derived from NJAU5773 and Zhongshuang 11. The <i>BnUD1</i> locus was initially mapped to a 3.99 Mb interval on the A05 chromosome with a BC<sub>6</sub>F<sub>2</sub> population by a bulked segregant analysis-sequencing approach. To more precisely map <i>BnUD1</i>, 103 InDel primer pairs uniformly covering the mapping interval and the BC<sub>5</sub>F<sub>3</sub> and BC<sub>6</sub>F<sub>2</sub> populations consisting of 1042 individuals were used to narrow the mapping interval to a 54.84 kb region. The mapping interval included 11 annotated genes. The bioinformatic analysis and gene sequencing data suggested that <i>BnaA05G0157900ZS</i> and <i>BnaA05G0158100ZS</i> may be responsible for the mutant traits. Protein sequence analyses showed that the mutations in the candidate gene <i>BnaA05G0157900ZS</i> altered the encoded PME in the trans-membrane region (G45A), the PMEI domain (G122S), and the pectinesterase domain (G394D). In addition, a 573 bp insertion was detected in the pectinesterase domain of the <i>BnaA05G0157900ZS</i> gene in the <i>Bnud1</i> mutant. Other primary experiments indicated that the locus responsible for the downward-pointing siliques and up-curling leaves negatively affected the plant height and 1000-seed weight, but it significantly increased the seeds per silique and positively affected photosynthetic efficiency to some extent. Furthermore, plants carrying the <i>BnUD1</i> locus were compact, implying they may be useful for increasing <i>B. napus</i> planting density. The findings of this study provide an important foundation for future research on the genetic mechanism regulating the dicotyledonous plant growth status, and the <i>Bnud1</i> plants can be used directly in breeding. |
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| ISSN: | 1661-6596 1422-0067 |