Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i>
Leaves and siliques are important organs associated with dry matter biosynthesis and vegetable oil accumulation in plants. We identified and characterized a novel locus controlling leaf and silique development using the <i>Brassica napus</i> mutant <i>Bnud1</i>, which has dow...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/4/3069 |
| _version_ | 1797620616207007744 |
|---|---|
| author | Mao Yang Jun Chen Yuqing Chang Shubei Wan Zisu Zhao Fei Ni Rongzhan Guan |
| author_facet | Mao Yang Jun Chen Yuqing Chang Shubei Wan Zisu Zhao Fei Ni Rongzhan Guan |
| author_sort | Mao Yang |
| collection | DOAJ |
| description | Leaves and siliques are important organs associated with dry matter biosynthesis and vegetable oil accumulation in plants. We identified and characterized a novel locus controlling leaf and silique development using the <i>Brassica napus</i> mutant <i>Bnud1</i>, which has downward-pointing siliques and up-curling leaves. The inheritance analysis showed that the up-curling leaf and downward-pointing silique traits are controlled by one dominant locus (<i>BnUD1</i>) in populations derived from NJAU5773 and Zhongshuang 11. The <i>BnUD1</i> locus was initially mapped to a 3.99 Mb interval on the A05 chromosome with a BC<sub>6</sub>F<sub>2</sub> population by a bulked segregant analysis-sequencing approach. To more precisely map <i>BnUD1</i>, 103 InDel primer pairs uniformly covering the mapping interval and the BC<sub>5</sub>F<sub>3</sub> and BC<sub>6</sub>F<sub>2</sub> populations consisting of 1042 individuals were used to narrow the mapping interval to a 54.84 kb region. The mapping interval included 11 annotated genes. The bioinformatic analysis and gene sequencing data suggested that <i>BnaA05G0157900ZS</i> and <i>BnaA05G0158100ZS</i> may be responsible for the mutant traits. Protein sequence analyses showed that the mutations in the candidate gene <i>BnaA05G0157900ZS</i> altered the encoded PME in the trans-membrane region (G45A), the PMEI domain (G122S), and the pectinesterase domain (G394D). In addition, a 573 bp insertion was detected in the pectinesterase domain of the <i>BnaA05G0157900ZS</i> gene in the <i>Bnud1</i> mutant. Other primary experiments indicated that the locus responsible for the downward-pointing siliques and up-curling leaves negatively affected the plant height and 1000-seed weight, but it significantly increased the seeds per silique and positively affected photosynthetic efficiency to some extent. Furthermore, plants carrying the <i>BnUD1</i> locus were compact, implying they may be useful for increasing <i>B. napus</i> planting density. The findings of this study provide an important foundation for future research on the genetic mechanism regulating the dicotyledonous plant growth status, and the <i>Bnud1</i> plants can be used directly in breeding. |
| first_indexed | 2024-03-11T08:45:06Z |
| format | Article |
| id | doaj.art-8f3c92a6bd24459589624cb6626feac9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:45:06Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8f3c92a6bd24459589624cb6626feac92023-11-16T20:54:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244306910.3390/ijms24043069Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i>Mao Yang0Jun Chen1Yuqing Chang2Shubei Wan3Zisu Zhao4Fei Ni5Rongzhan Guan6State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaLeaves and siliques are important organs associated with dry matter biosynthesis and vegetable oil accumulation in plants. We identified and characterized a novel locus controlling leaf and silique development using the <i>Brassica napus</i> mutant <i>Bnud1</i>, which has downward-pointing siliques and up-curling leaves. The inheritance analysis showed that the up-curling leaf and downward-pointing silique traits are controlled by one dominant locus (<i>BnUD1</i>) in populations derived from NJAU5773 and Zhongshuang 11. The <i>BnUD1</i> locus was initially mapped to a 3.99 Mb interval on the A05 chromosome with a BC<sub>6</sub>F<sub>2</sub> population by a bulked segregant analysis-sequencing approach. To more precisely map <i>BnUD1</i>, 103 InDel primer pairs uniformly covering the mapping interval and the BC<sub>5</sub>F<sub>3</sub> and BC<sub>6</sub>F<sub>2</sub> populations consisting of 1042 individuals were used to narrow the mapping interval to a 54.84 kb region. The mapping interval included 11 annotated genes. The bioinformatic analysis and gene sequencing data suggested that <i>BnaA05G0157900ZS</i> and <i>BnaA05G0158100ZS</i> may be responsible for the mutant traits. Protein sequence analyses showed that the mutations in the candidate gene <i>BnaA05G0157900ZS</i> altered the encoded PME in the trans-membrane region (G45A), the PMEI domain (G122S), and the pectinesterase domain (G394D). In addition, a 573 bp insertion was detected in the pectinesterase domain of the <i>BnaA05G0157900ZS</i> gene in the <i>Bnud1</i> mutant. Other primary experiments indicated that the locus responsible for the downward-pointing siliques and up-curling leaves negatively affected the plant height and 1000-seed weight, but it significantly increased the seeds per silique and positively affected photosynthetic efficiency to some extent. Furthermore, plants carrying the <i>BnUD1</i> locus were compact, implying they may be useful for increasing <i>B. napus</i> planting density. The findings of this study provide an important foundation for future research on the genetic mechanism regulating the dicotyledonous plant growth status, and the <i>Bnud1</i> plants can be used directly in breeding.https://www.mdpi.com/1422-0067/24/4/3069<i>Brassica napus</i>downward-pointing siliquegene mappingup-curling leaf |
| spellingShingle | Mao Yang Jun Chen Yuqing Chang Shubei Wan Zisu Zhao Fei Ni Rongzhan Guan Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i> International Journal of Molecular Sciences <i>Brassica napus</i> downward-pointing silique gene mapping up-curling leaf |
| title | Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i> |
| title_full | Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i> |
| title_fullStr | Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i> |
| title_full_unstemmed | Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i> |
| title_short | Fine Mapping of a Pleiotropic Locus (<i>BnUD1</i>) Responsible for the Up-Curling Leaves and Downward-Pointing Siliques in <i>Brassica napus</i> |
| title_sort | fine mapping of a pleiotropic locus i bnud1 i responsible for the up curling leaves and downward pointing siliques in i brassica napus i |
| topic | <i>Brassica napus</i> downward-pointing silique gene mapping up-curling leaf |
| url | https://www.mdpi.com/1422-0067/24/4/3069 |
| work_keys_str_mv | AT maoyang finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi AT junchen finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi AT yuqingchang finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi AT shubeiwan finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi AT zisuzhao finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi AT feini finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi AT rongzhanguan finemappingofapleiotropiclocusibnud1iresponsiblefortheupcurlingleavesanddownwardpointingsiliquesinibrassicanapusi |