Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy
In this study, we compare the vitreous cytokine profile in patients with proliferative diabetic retinopathy (PDR) to that of patients without PDR. The identification of novel cytokines involved in the pathogenesis of PDR provides candidate therapeutic targets that may stand alone or work synergistic...
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MDPI AG
2021-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/5/1069 |
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| author | Dean F. Loporchio Emily K. Tam Jane Cho Jaeyoon Chung Gyungah R. Jun Weiming Xia Marissa G. Fiorello Nicole H. Siegel Steven Ness Thor D. Stein Manju L. Subramanian |
| author_facet | Dean F. Loporchio Emily K. Tam Jane Cho Jaeyoon Chung Gyungah R. Jun Weiming Xia Marissa G. Fiorello Nicole H. Siegel Steven Ness Thor D. Stein Manju L. Subramanian |
| author_sort | Dean F. Loporchio |
| collection | DOAJ |
| description | In this study, we compare the vitreous cytokine profile in patients with proliferative diabetic retinopathy (PDR) to that of patients without PDR. The identification of novel cytokines involved in the pathogenesis of PDR provides candidate therapeutic targets that may stand alone or work synergistically with current therapies in the management of diabetic retinopathy. Undiluted vitreous humor specimens were collected from 74 patients undergoing vitrectomy for various vitreoretinal disorders. Quantitative immunoassay was performed for a panel of 36 neuroinflammatory cytokines in each specimen and assessed to identify differences between PDR (<i>n</i> = 35) and non-PDR (<i>n</i> = 39) patients. Levels of interleukin-8 (IL-8), IL-15, IL-16, vascular endothelial growth factor (VEGF), VEGF-D, c-reactive protein (CRP), serum amyloid-A (SAA), and intracellular adhesion molecule-1 (ICAM1) were significantly increased in the vitreous of PDR patients compared to non-PDR patients (<i>p</i> < 0.05). We report novel increases in IL-15 and IL-16, in addition to the expected VEGF, in the human vitreous humor of patients with PDR. Additionally, we confirm the elevation of ICAM-1, VCAM-1, SAA, IL-8 and CRP in the vitreous of patients with PDR, which has previously been described. |
| first_indexed | 2024-03-10T11:47:19Z |
| format | Article |
| id | doaj.art-8f48d3d97b7942bb9152e9be87245603 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T11:47:19Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-8f48d3d97b7942bb9152e9be872456032023-11-21T17:59:20ZengMDPI AGCells2073-44092021-04-01105106910.3390/cells10051069Cytokine Levels in Human Vitreous in Proliferative Diabetic RetinopathyDean F. Loporchio0Emily K. Tam1Jane Cho2Jaeyoon Chung3Gyungah R. Jun4Weiming Xia5Marissa G. Fiorello6Nicole H. Siegel7Steven Ness8Thor D. Stein9Manju L. Subramanian10Department of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USADepartment of Medicine (Biomedical Genetics), Boston University School of Medicine, 72 East Concord Street, Boston, MA 02118, USADepartment of Medicine (Biomedical Genetics), Boston University School of Medicine, 72 East Concord Street, Boston, MA 02118, USADepartment of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USALaboratory Medicine, Department of Pathology, Boston University School of Medicine, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USAIn this study, we compare the vitreous cytokine profile in patients with proliferative diabetic retinopathy (PDR) to that of patients without PDR. The identification of novel cytokines involved in the pathogenesis of PDR provides candidate therapeutic targets that may stand alone or work synergistically with current therapies in the management of diabetic retinopathy. Undiluted vitreous humor specimens were collected from 74 patients undergoing vitrectomy for various vitreoretinal disorders. Quantitative immunoassay was performed for a panel of 36 neuroinflammatory cytokines in each specimen and assessed to identify differences between PDR (<i>n</i> = 35) and non-PDR (<i>n</i> = 39) patients. Levels of interleukin-8 (IL-8), IL-15, IL-16, vascular endothelial growth factor (VEGF), VEGF-D, c-reactive protein (CRP), serum amyloid-A (SAA), and intracellular adhesion molecule-1 (ICAM1) were significantly increased in the vitreous of PDR patients compared to non-PDR patients (<i>p</i> < 0.05). We report novel increases in IL-15 and IL-16, in addition to the expected VEGF, in the human vitreous humor of patients with PDR. Additionally, we confirm the elevation of ICAM-1, VCAM-1, SAA, IL-8 and CRP in the vitreous of patients with PDR, which has previously been described.https://www.mdpi.com/2073-4409/10/5/1069diabetic retinopathyvitreous cytokinesquantitative immunoassayneuroinflammatory markersvascular endothelial growth factorinterleukin-8 |
| spellingShingle | Dean F. Loporchio Emily K. Tam Jane Cho Jaeyoon Chung Gyungah R. Jun Weiming Xia Marissa G. Fiorello Nicole H. Siegel Steven Ness Thor D. Stein Manju L. Subramanian Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy Cells diabetic retinopathy vitreous cytokines quantitative immunoassay neuroinflammatory markers vascular endothelial growth factor interleukin-8 |
| title | Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy |
| title_full | Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy |
| title_fullStr | Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy |
| title_full_unstemmed | Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy |
| title_short | Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy |
| title_sort | cytokine levels in human vitreous in proliferative diabetic retinopathy |
| topic | diabetic retinopathy vitreous cytokines quantitative immunoassay neuroinflammatory markers vascular endothelial growth factor interleukin-8 |
| url | https://www.mdpi.com/2073-4409/10/5/1069 |
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