Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines
Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-co...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2023-03-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/41079 |
| _version_ | 1811159502525825024 |
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| author | Omat Rachmat Hasbullah Ilma Fiddiyanti Dewi Ratih Handayani Endang Sutedja Darmadji Ismono Nucki Nursjamsi Hidajat Wahyu Widowati Ervi Afifah Hanna Sari Widya Kusuma Rizal Rizal Firman Alamsyah Warsito P. Taruno |
| author_facet | Omat Rachmat Hasbullah Ilma Fiddiyanti Dewi Ratih Handayani Endang Sutedja Darmadji Ismono Nucki Nursjamsi Hidajat Wahyu Widowati Ervi Afifah Hanna Sari Widya Kusuma Rizal Rizal Firman Alamsyah Warsito P. Taruno |
| author_sort | Omat Rachmat Hasbullah |
| collection | DOAJ |
| description |
Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-contact electric field has anti-proliferative effect on breast cancer cells, in vitro and in vivo. In this study, we were interested studying alternating current electric field effects on osteosarcoma cells progression as well as its potential cytotoxic effects. MG-63 human osteosarcoma cells were cultured and treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, and caspase-9 were analyzed using real-time qPCR method. Apoptotic index was measured using flow-cytometry assay. Apoptosis was observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis through internal pathways were shown by the increase of caspase-3 (p = 0.015) and caspase-9 (p = 0.001) levels, but not caspase-8 (p = 0.080). This treatment has successfully reduced the number of living osteosarcoma cells by 14.7% (p = 0.000) and increased cell death up to 4.26% (p = 0.055). Apoptotic index was markedly increased to 16% (p = 0.001). 200 kHz non-contact electric field exposure can disrupt osteosarcoma progression through disruption of normal cell cycle via p53-independent p21 pathway and induction of apoptosis.
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| first_indexed | 2024-04-10T05:42:17Z |
| format | Article |
| id | doaj.art-8f49eda8a9a64c33894121b76f5d7db6 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-10T05:42:17Z |
| publishDate | 2023-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-8f49eda8a9a64c33894121b76f5d7db62023-03-06T07:57:44ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942023-03-0130310.4308/hjb.30.3.522-531Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell LinesOmat Rachmat Hasbullah0Ilma Fiddiyanti1Dewi Ratih Handayani2Endang Sutedja3Darmadji Ismono4Nucki Nursjamsi Hidajat5Wahyu Widowati6Ervi Afifah7Hanna Sari Widya Kusuma8Rizal Rizal9Firman Alamsyah10Warsito P. Taruno11Faculty of Medicine, University of Sultan Ageng Tirtayasa, Serang, Banten 42124, IndonesiaFaculty of Medicine, University of Jenderal Achmad Yani, Cimahi 40531, IndonesiaFaculty of Medicine, University of Jenderal Achmad Yani, Cimahi 40531, IndonesiaFaculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, IndonesiaFaculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, IndonesiaFaculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, IndonesiaFaculty of Medicine, Maranatha Christian University, Bandung 40164, IndonesiaBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung 40163, IndonesiaBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung 40163, IndonesiaBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung 40163, Indonesia. Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, IndonesiaCenter for Medical Physics and Cancer Research Ctech Labs Edwar Technology, Tangerang 15143, IndonesiaCenter for Medical Physics and Cancer Research Ctech Labs Edwar Technology, Tangerang 15143, Indonesia Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-contact electric field has anti-proliferative effect on breast cancer cells, in vitro and in vivo. In this study, we were interested studying alternating current electric field effects on osteosarcoma cells progression as well as its potential cytotoxic effects. MG-63 human osteosarcoma cells were cultured and treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, and caspase-9 were analyzed using real-time qPCR method. Apoptotic index was measured using flow-cytometry assay. Apoptosis was observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis through internal pathways were shown by the increase of caspase-3 (p = 0.015) and caspase-9 (p = 0.001) levels, but not caspase-8 (p = 0.080). This treatment has successfully reduced the number of living osteosarcoma cells by 14.7% (p = 0.000) and increased cell death up to 4.26% (p = 0.055). Apoptotic index was markedly increased to 16% (p = 0.001). 200 kHz non-contact electric field exposure can disrupt osteosarcoma progression through disruption of normal cell cycle via p53-independent p21 pathway and induction of apoptosis. https://journal.ipb.ac.id/index.php/hayati/article/view/41079 |
| spellingShingle | Omat Rachmat Hasbullah Ilma Fiddiyanti Dewi Ratih Handayani Endang Sutedja Darmadji Ismono Nucki Nursjamsi Hidajat Wahyu Widowati Ervi Afifah Hanna Sari Widya Kusuma Rizal Rizal Firman Alamsyah Warsito P. Taruno Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines Hayati Journal of Biosciences |
| title | Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines |
| title_full | Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines |
| title_fullStr | Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines |
| title_full_unstemmed | Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines |
| title_short | Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines |
| title_sort | non contact electric field exposure provides potential cancer therapy through p53 independent proliferation arrest and intrinsic pathway apoptosis induction in mg 63 cell lines |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/41079 |
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