Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos
Objective: To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods: Two experiments were performed, the first one aimed to evaluate the differ...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2017-01-01
|
| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=2;spage=85;epage=88;aulast=l-Naby |
| _version_ | 1828200536965382144 |
|---|---|
| author | Al-shimaa Al-H H l-Naby KarimaGh M Mahmoud Gamal AM Sosa Mahmoud EA Abouel-Roos Youssef F Ahmed |
| author_facet | Al-shimaa Al-H H l-Naby KarimaGh M Mahmoud Gamal AM Sosa Mahmoud EA Abouel-Roos Youssef F Ahmed |
| author_sort | Al-shimaa Al-H H l-Naby |
| collection | DOAJ |
| description | Objective: To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods: Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid + cysteamine) or ascorbic acid only.Results: There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved embryo development than ascorbic acid alone.Conclusions: Using of 50 μM L-ascorbic acid during in vitro maturation and development improve the developmental competence of buffalo oocytes, this effect was increase with the presence of cysteamine. |
| first_indexed | 2024-04-12T11:14:49Z |
| format | Article |
| id | doaj.art-8f4d044525424408b2f64376bb12ed55 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 2305-0519 |
| language | English |
| last_indexed | 2024-04-12T11:14:49Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-8f4d044525424408b2f64376bb12ed552022-12-22T03:35:31ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192017-01-0162858810.12980/apjr.6.20170207Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryosAl-shimaa Al-H H l-NabyKarimaGh M MahmoudGamal AM SosaMahmoud EA Abouel-RoosYoussef F AhmedObjective: To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods: Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid + cysteamine) or ascorbic acid only.Results: There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved embryo development than ascorbic acid alone.Conclusions: Using of 50 μM L-ascorbic acid during in vitro maturation and development improve the developmental competence of buffalo oocytes, this effect was increase with the presence of cysteamine.http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=2;spage=85;epage=88;aulast=l-NabyBuffaloOocytesL-ascorbic acidCysteamineEmbryo development |
| spellingShingle | Al-shimaa Al-H H l-Naby KarimaGh M Mahmoud Gamal AM Sosa Mahmoud EA Abouel-Roos Youssef F Ahmed Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos Asian Pacific Journal of Reproduction Buffalo Oocytes L-ascorbic acid Cysteamine Embryo development |
| title | Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos |
| title_full | Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos |
| title_fullStr | Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos |
| title_full_unstemmed | Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos |
| title_short | Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos |
| title_sort | effect of using ascorbic acid and cysteamine supplementation on in vitro development of buffalo embryos |
| topic | Buffalo Oocytes L-ascorbic acid Cysteamine Embryo development |
| url | http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=2;spage=85;epage=88;aulast=l-Naby |
| work_keys_str_mv | AT alshimaaalhhlnaby effectofusingascorbicacidandcysteaminesupplementationoninvitrodevelopmentofbuffaloembryos AT karimaghmmahmoud effectofusingascorbicacidandcysteaminesupplementationoninvitrodevelopmentofbuffaloembryos AT gamalamsosa effectofusingascorbicacidandcysteaminesupplementationoninvitrodevelopmentofbuffaloembryos AT mahmoudeaabouelroos effectofusingascorbicacidandcysteaminesupplementationoninvitrodevelopmentofbuffaloembryos AT yousseffahmed effectofusingascorbicacidandcysteaminesupplementationoninvitrodevelopmentofbuffaloembryos |