Sequence-based prediction of permissive stretches for internal protein tagging and knockdown
Abstract Background Internal tagging of proteins by inserting small functional peptides into surface accessible permissive sites has proven to be an indispensable tool for basic and applied science. Permissive sites are typically identified by transposon mutagenesis on a case-by-case basis, limiting...
Main Authors: | Sabine Oesterle, Tania Michelle Roberts, Lukas Andreas Widmer, Harun Mustafa, Sven Panke, Sonja Billerbeck |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2017-10-01
|
Series: | BMC Biology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12915-017-0440-0 |
Similar Items
-
A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression
by: Szilvia Krisztina Nagy, et al.
Published: (2020-03-01) -
Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct
by: Pragyan P. Parida, et al.
Published: (2023-01-01) -
Going native: Complete removal of protein purification affinity tags by simple modification of existing tags and proteases
by: Goh, Hui Chin, et al.
Published: (2017) -
Engineering a switchable single‐chain TEV protease to control protein maturation in living neurons
by: Pietro Renna, et al.
Published: (2022-05-01) -
Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in <i>Escherichia coli</i>, Purification, and Characterization
by: Monika Cserjan-Puschmann, et al.
Published: (2020-11-01)