Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue
Abstract Background The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4410-x |
| _version_ | 1811325543424983040 |
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| author | Mylène A. Carrascal Catarina Talina Paula Borralho A. Gonçalo Mineiro Ana Raquel Henriques Cláudia Pen Manuela Martins Sofia Braga Robert Sackstein Paula A. Videira |
| author_facet | Mylène A. Carrascal Catarina Talina Paula Borralho A. Gonçalo Mineiro Ana Raquel Henriques Cláudia Pen Manuela Martins Sofia Braga Robert Sackstein Paula A. Videira |
| author_sort | Mylène A. Carrascal |
| collection | DOAJ |
| description | Abstract Background The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield new biomarkers for patient stratification and aid in identifying novel therapeutic targets. The determinants of selectin ligands consist of sialylated tetrasaccharides, the sialyl Lewis X and A (sLeX and sLeA), displayed on protein or lipid scaffolds. Standardized procedures for immunohistochemistry make use of the antibodies against sLeX and/or sLeA. However, antibody binding does not define E-selectin binding activity. Methods In this study, we developed an immunohistochemical staining technique, using E-selectin-human Ig Fc chimera (E-Ig) to characterize the expression and localization of E-selectin binding sites on paraffin-embedded sections of different cancer tissue. Results E-Ig successfully stained cancer cells with high specificity. The E-Ig staining show high reactivity scores in colon and lung adenocarcinoma and moderate reactivity in triple negative breast cancer. Compared with reactivity of antibody against sLeX/A, the E-Ig staining presented higher specificity to cancer tissue with better defined borders and less background. Conclusions The E-Ig staining technique allows the qualitative and semi-quantitative analysis of E-selectin binding activity on cancer cells. The development of accurate techniques for detection of selectin ligands may contribute to better diagnostic and better understanding of the molecular basis of tumor progression and metastasis. |
| first_indexed | 2024-04-13T14:35:03Z |
| format | Article |
| id | doaj.art-8f55e977b4ed44ed8949fa74815c40fc |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T14:35:03Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-8f55e977b4ed44ed8949fa74815c40fc2022-12-22T02:43:03ZengBMCBMC Cancer1471-24072018-05-0118111010.1186/s12885-018-4410-xStaining of E-selectin ligands on paraffin-embedded sections of tumor tissueMylène A. Carrascal0Catarina Talina1Paula Borralho2A. Gonçalo Mineiro3Ana Raquel Henriques4Cláudia Pen5Manuela Martins6Sofia Braga7Robert Sackstein8Paula A. Videira9UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de LisboaCEDOC, Chronic Diseases Research Center, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de LisboaCEDOC, Chronic Diseases Research Center, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de LisboaUCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de LisboaCEDOC, Chronic Diseases Research Center, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de LisboaCentro Hospitalar de Lisboa Central, EPE e Serviço de Anatomia PatológicaCentro Hospitalar de Lisboa Central, EPE e Serviço de Anatomia PatológicaHospital CUF DescobertasDepartments of Dermatology and Medicine, Brigham & Women’s Hospital, Harvard Medical SchoolUCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de LisboaAbstract Background The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield new biomarkers for patient stratification and aid in identifying novel therapeutic targets. The determinants of selectin ligands consist of sialylated tetrasaccharides, the sialyl Lewis X and A (sLeX and sLeA), displayed on protein or lipid scaffolds. Standardized procedures for immunohistochemistry make use of the antibodies against sLeX and/or sLeA. However, antibody binding does not define E-selectin binding activity. Methods In this study, we developed an immunohistochemical staining technique, using E-selectin-human Ig Fc chimera (E-Ig) to characterize the expression and localization of E-selectin binding sites on paraffin-embedded sections of different cancer tissue. Results E-Ig successfully stained cancer cells with high specificity. The E-Ig staining show high reactivity scores in colon and lung adenocarcinoma and moderate reactivity in triple negative breast cancer. Compared with reactivity of antibody against sLeX/A, the E-Ig staining presented higher specificity to cancer tissue with better defined borders and less background. Conclusions The E-Ig staining technique allows the qualitative and semi-quantitative analysis of E-selectin binding activity on cancer cells. The development of accurate techniques for detection of selectin ligands may contribute to better diagnostic and better understanding of the molecular basis of tumor progression and metastasis.http://link.springer.com/article/10.1186/s12885-018-4410-xE-selectin ligandsSialyl-Lewis XSialyl-Lewis aCancer |
| spellingShingle | Mylène A. Carrascal Catarina Talina Paula Borralho A. Gonçalo Mineiro Ana Raquel Henriques Cláudia Pen Manuela Martins Sofia Braga Robert Sackstein Paula A. Videira Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue BMC Cancer E-selectin ligands Sialyl-Lewis X Sialyl-Lewis a Cancer |
| title | Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue |
| title_full | Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue |
| title_fullStr | Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue |
| title_full_unstemmed | Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue |
| title_short | Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue |
| title_sort | staining of e selectin ligands on paraffin embedded sections of tumor tissue |
| topic | E-selectin ligands Sialyl-Lewis X Sialyl-Lewis a Cancer |
| url | http://link.springer.com/article/10.1186/s12885-018-4410-x |
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