Significance of urinary full-length megalin in patients with IgA nephropathy.
BACKGROUND AND OBJECTIVES: Megalin is highly expressed at the apical membranes of proximal tubular epithelial cells. A urinary full-length megalin (C-megalin) assay is linked to the severity of diabetic nephropathy in type 2 diabetes. This study examined the relationship between levels of urinary C-...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4264761?pdf=render |
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| author | Takuto Seki Katsuhiko Asanuma Rin Asao Kanae Nonaka Yu Sasaki Juan Alejandro Oliva Trejo Hiroyuki Kurosawa Yoshiaki Hirayama Satoshi Horikoshi Yasuhiko Tomino Akihiko Saito |
| author_facet | Takuto Seki Katsuhiko Asanuma Rin Asao Kanae Nonaka Yu Sasaki Juan Alejandro Oliva Trejo Hiroyuki Kurosawa Yoshiaki Hirayama Satoshi Horikoshi Yasuhiko Tomino Akihiko Saito |
| author_sort | Takuto Seki |
| collection | DOAJ |
| description | BACKGROUND AND OBJECTIVES: Megalin is highly expressed at the apical membranes of proximal tubular epithelial cells. A urinary full-length megalin (C-megalin) assay is linked to the severity of diabetic nephropathy in type 2 diabetes. This study examined the relationship between levels of urinary C-megalin and histological findings in adult patients with IgA nephropathy (IgAN). DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Urine samples voided in the morning on the day of renal biopsy were obtained from 73 patients with IgAN (29 men and 44 women; mean age, 33 years) and 5 patients with membranous nephropathy (MN). Renal pathologic variables were analyzed using the Oxford classification of IgAN, the Shigematsu classification and the Clinical Guidelines of IgAN in Japan. The levels of urinary C-megalin were measured by sandwich ELISA. RESULTS: Histological analysis based on the Oxford classification revealed that the levels of urinary C-megalin were correlated with mesangial hypercellularity in IgAN patients (OR = 1.76, 95% CI: 1.04-3.27, P<0.05). There was a significant correlation between the levels of urinary C-megalin and the severity of chronic extracapillary abnormalities according to the Shigematsu classification in IgAN patients (β = 0.33, P = 0.008). The levels of urinary C-megalin were significantly higher in all risk levels of IgAN patients requiring dialysis using the Clinical Guidelines of IgAN in Japan than in the control group. The levels of urinary C-megalin were significantly higher in the high risk and very high risk grades than in the low risk grade (P<0.05). The levels of urinary C-megalin were significantly higher in MN patients compared to the control group. CONCLUSIONS: The levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients. There is a possibility that urinary C-megalin is an independent predictor of disease progression of IgAN. In addition, our results suggest that urinary C-megalin is a marker of glomerular abnormalities in various glomerular diseases as well as IgAN. |
| first_indexed | 2024-04-12T18:38:49Z |
| format | Article |
| id | doaj.art-8f571254781548b99ea04e1497d4a3d2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T18:38:49Z |
| publishDate | 2014-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-8f571254781548b99ea04e1497d4a3d22022-12-22T03:20:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11440010.1371/journal.pone.0114400Significance of urinary full-length megalin in patients with IgA nephropathy.Takuto SekiKatsuhiko AsanumaRin AsaoKanae NonakaYu SasakiJuan Alejandro Oliva TrejoHiroyuki KurosawaYoshiaki HirayamaSatoshi HorikoshiYasuhiko TominoAkihiko SaitoBACKGROUND AND OBJECTIVES: Megalin is highly expressed at the apical membranes of proximal tubular epithelial cells. A urinary full-length megalin (C-megalin) assay is linked to the severity of diabetic nephropathy in type 2 diabetes. This study examined the relationship between levels of urinary C-megalin and histological findings in adult patients with IgA nephropathy (IgAN). DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Urine samples voided in the morning on the day of renal biopsy were obtained from 73 patients with IgAN (29 men and 44 women; mean age, 33 years) and 5 patients with membranous nephropathy (MN). Renal pathologic variables were analyzed using the Oxford classification of IgAN, the Shigematsu classification and the Clinical Guidelines of IgAN in Japan. The levels of urinary C-megalin were measured by sandwich ELISA. RESULTS: Histological analysis based on the Oxford classification revealed that the levels of urinary C-megalin were correlated with mesangial hypercellularity in IgAN patients (OR = 1.76, 95% CI: 1.04-3.27, P<0.05). There was a significant correlation between the levels of urinary C-megalin and the severity of chronic extracapillary abnormalities according to the Shigematsu classification in IgAN patients (β = 0.33, P = 0.008). The levels of urinary C-megalin were significantly higher in all risk levels of IgAN patients requiring dialysis using the Clinical Guidelines of IgAN in Japan than in the control group. The levels of urinary C-megalin were significantly higher in the high risk and very high risk grades than in the low risk grade (P<0.05). The levels of urinary C-megalin were significantly higher in MN patients compared to the control group. CONCLUSIONS: The levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients. There is a possibility that urinary C-megalin is an independent predictor of disease progression of IgAN. In addition, our results suggest that urinary C-megalin is a marker of glomerular abnormalities in various glomerular diseases as well as IgAN.http://europepmc.org/articles/PMC4264761?pdf=render |
| spellingShingle | Takuto Seki Katsuhiko Asanuma Rin Asao Kanae Nonaka Yu Sasaki Juan Alejandro Oliva Trejo Hiroyuki Kurosawa Yoshiaki Hirayama Satoshi Horikoshi Yasuhiko Tomino Akihiko Saito Significance of urinary full-length megalin in patients with IgA nephropathy. PLoS ONE |
| title | Significance of urinary full-length megalin in patients with IgA nephropathy. |
| title_full | Significance of urinary full-length megalin in patients with IgA nephropathy. |
| title_fullStr | Significance of urinary full-length megalin in patients with IgA nephropathy. |
| title_full_unstemmed | Significance of urinary full-length megalin in patients with IgA nephropathy. |
| title_short | Significance of urinary full-length megalin in patients with IgA nephropathy. |
| title_sort | significance of urinary full length megalin in patients with iga nephropathy |
| url | http://europepmc.org/articles/PMC4264761?pdf=render |
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