De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy
Abstract Background Aromatic compounds, such as p-coumaric acid (p-CA) and caffeic acid, are secondary metabolites of various plants, and are widely used in diet and industry for their biological activities. In addition to expensive and unsustainable methods of plant extraction and chemical synthesi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-01805-5 |
| _version_ | 1818203664849829888 |
|---|---|
| author | Miao Cai Jiayu Liu Xiaofei Song Hang Qi Yuanzi Li Zhenzhou Wu Haijin Xu Mingqiang Qiao |
| author_facet | Miao Cai Jiayu Liu Xiaofei Song Hang Qi Yuanzi Li Zhenzhou Wu Haijin Xu Mingqiang Qiao |
| author_sort | Miao Cai |
| collection | DOAJ |
| description | Abstract Background Aromatic compounds, such as p-coumaric acid (p-CA) and caffeic acid, are secondary metabolites of various plants, and are widely used in diet and industry for their biological activities. In addition to expensive and unsustainable methods of plant extraction and chemical synthesis, the strategy for heterologous synthesis of aromatic compounds in microorganisms has received much attention. As the most abundant renewable resource in the world, lignocellulose is an economical and environmentally friendly alternative to edible, high-cost carbon sources such as glucose. Results In the present study, carboxymethyl-cellulose (CMC) was utilized as the sole carbon source, and a metabolically engineered Saccharomyces cerevisiae strain SK10-3 was co-cultured with other recombinant S. cerevisiae strains to achieve the bioconversion of value-added products from CMC. By optimizing the inoculation ratio, interval time, and carbon source content, the final titer of p-CA in 30 g/L CMC medium was increased to 71.71 mg/L, which was 155.9-fold higher than that achieved in mono-culture. The de novo biosynthesis of caffeic acid in the CMC medium was also achieved through a three-strain co-cultivation. Caffeic acid production was up to 16.91 mg/L after optimizing the inoculation ratio of these strains. Conclusion De novo biosynthesis of p-CA and caffeic acid from lignocellulose through a co-cultivation strategy was achieved for the first time. This study provides favorable support for the biosynthesis of more high value-added products from economical substrates. In addition, the multi-strain co-culture strategy can effectively improve the final titer of the target products, which has high application potential in the field of industrial production. |
| first_indexed | 2024-12-12T03:28:57Z |
| format | Article |
| id | doaj.art-8f58539cc7504a87aae115738e5f55e8 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-12T03:28:57Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-8f58539cc7504a87aae115738e5f55e82022-12-22T00:39:58ZengBMCMicrobial Cell Factories1475-28592022-05-0121111210.1186/s12934-022-01805-5De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategyMiao Cai0Jiayu Liu1Xiaofei Song2Hang Qi3Yuanzi Li4Zhenzhou Wu5Haijin Xu6Mingqiang Qiao7The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityCollege Biotechnology and Bioengineering, Zhejiang University of TechnologyThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversitySchool of Light Industry, Beijing Technology and Business University (BTBU)The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai UniversityAbstract Background Aromatic compounds, such as p-coumaric acid (p-CA) and caffeic acid, are secondary metabolites of various plants, and are widely used in diet and industry for their biological activities. In addition to expensive and unsustainable methods of plant extraction and chemical synthesis, the strategy for heterologous synthesis of aromatic compounds in microorganisms has received much attention. As the most abundant renewable resource in the world, lignocellulose is an economical and environmentally friendly alternative to edible, high-cost carbon sources such as glucose. Results In the present study, carboxymethyl-cellulose (CMC) was utilized as the sole carbon source, and a metabolically engineered Saccharomyces cerevisiae strain SK10-3 was co-cultured with other recombinant S. cerevisiae strains to achieve the bioconversion of value-added products from CMC. By optimizing the inoculation ratio, interval time, and carbon source content, the final titer of p-CA in 30 g/L CMC medium was increased to 71.71 mg/L, which was 155.9-fold higher than that achieved in mono-culture. The de novo biosynthesis of caffeic acid in the CMC medium was also achieved through a three-strain co-cultivation. Caffeic acid production was up to 16.91 mg/L after optimizing the inoculation ratio of these strains. Conclusion De novo biosynthesis of p-CA and caffeic acid from lignocellulose through a co-cultivation strategy was achieved for the first time. This study provides favorable support for the biosynthesis of more high value-added products from economical substrates. In addition, the multi-strain co-culture strategy can effectively improve the final titer of the target products, which has high application potential in the field of industrial production.https://doi.org/10.1186/s12934-022-01805-5Saccharomyces cerevisiaeCo-cultureCarboxymethyl-cellulosep-coumaric acidCaffeic acid |
| spellingShingle | Miao Cai Jiayu Liu Xiaofei Song Hang Qi Yuanzi Li Zhenzhou Wu Haijin Xu Mingqiang Qiao De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy Microbial Cell Factories Saccharomyces cerevisiae Co-culture Carboxymethyl-cellulose p-coumaric acid Caffeic acid |
| title | De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy |
| title_full | De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy |
| title_fullStr | De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy |
| title_full_unstemmed | De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy |
| title_short | De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy |
| title_sort | de novo biosynthesis of p coumaric acid and caffeic acid from carboxymethyl cellulose by microbial co culture strategy |
| topic | Saccharomyces cerevisiae Co-culture Carboxymethyl-cellulose p-coumaric acid Caffeic acid |
| url | https://doi.org/10.1186/s12934-022-01805-5 |
| work_keys_str_mv | AT miaocai denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT jiayuliu denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT xiaofeisong denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT hangqi denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT yuanzili denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT zhenzhouwu denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT haijinxu denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy AT mingqiangqiao denovobiosynthesisofpcoumaricacidandcaffeicacidfromcarboxymethylcellulosebymicrobialcoculturestrategy |