A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain
Fusarium head blight (FHB), caused by Fusarium spp., is a destructive disease of cereal grains. Apart from grain yield loss, a major quality concern is contamination with Fusarium-produced mycotoxins, specifically deoxynivalenol (DON). Mycotoxins accumulate in the grain, making it unfit for consumpt...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2022-12-01
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| Series: | PhytoFrontiers |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-03-22-0024-TA |
| _version_ | 1827095685667749888 |
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| author | Lipu Wang Deborah Michel Wentao Zhang Anas El-Aneed Pierre R. Fobert Yuefeng Ruan Samia Berraies Richard Cuthbert Hadley R. Kutcher |
| author_facet | Lipu Wang Deborah Michel Wentao Zhang Anas El-Aneed Pierre R. Fobert Yuefeng Ruan Samia Berraies Richard Cuthbert Hadley R. Kutcher |
| author_sort | Lipu Wang |
| collection | DOAJ |
| description | Fusarium head blight (FHB), caused by Fusarium spp., is a destructive disease of cereal grains. Apart from grain yield loss, a major quality concern is contamination with Fusarium-produced mycotoxins, specifically deoxynivalenol (DON). Mycotoxins accumulate in the grain, making it unfit for consumption by humans and animals. Breeding cultivars with high disease resistance and low mycotoxin contamination is a priority for wheat breeders. However, DON measurement in breeding programs is expensive and time consuming due to the lack of efficient quantification methods. In this study, we established a simple fast chromatography-tandem mass spectrometry method, which employed a one-step acetonitrile extraction protocol with a short guard column to reduce complexity, cost, and analysis time. To ensure robustness and reproducibility, the method was validated according to the U.S. Food and Drug Administration Guidance for Bioanalytical Method Validation. Furthermore, the method was applied for determination of DON in 102 wheat grain samples. Obtained results highly correlated with the conventional immunological method for all tested samples. With its ease of use, rapid sample analysis, and high sensitivity and accuracy, the method could be integrated into current FHB breeding programs to increase breeding efficiency and accelerate screening progress to identify germplasm with increased resistance to DON accumulation. [Figure: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| first_indexed | 2024-03-08T06:36:30Z |
| format | Article |
| id | doaj.art-8f58f9d6581e4ace828cdaa5edf15b62 |
| institution | Directory Open Access Journal |
| issn | 2690-5442 |
| language | English |
| last_indexed | 2025-03-20T07:03:49Z |
| publishDate | 2022-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | PhytoFrontiers |
| spelling | doaj.art-8f58f9d6581e4ace828cdaa5edf15b622024-10-01T12:35:40ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422022-12-012432233010.1094/PHYTOFR-03-22-0024-TAA High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat GrainLipu Wang0Deborah Michel1Wentao Zhang2Anas El-Aneed3Pierre R. Fobert4Yuefeng Ruan5Samia Berraies6Richard Cuthbert7Hadley R. Kutcher8Crop Development Centre/Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, CanadaCollege of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, S7N 5E5, CanadaAquatic and Crop Resources Development, National Research Council Canada, Saskatoon, SK, S7N 0W9, CanadaCollege of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, S7N 5E5, CanadaAquatic and Crop Resources Development, National Research Council Canada, Ottawa, ON, K1A 0R6, CanadaAgriculture and Agri-Food Canada, Swift Current Research and Development Centre, SK, S9H 3X2, CanadaAgriculture and Agri-Food Canada, Swift Current Research and Development Centre, SK, S9H 3X2, CanadaAgriculture and Agri-Food Canada, Swift Current Research and Development Centre, SK, S9H 3X2, CanadaCrop Development Centre/Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, CanadaFusarium head blight (FHB), caused by Fusarium spp., is a destructive disease of cereal grains. Apart from grain yield loss, a major quality concern is contamination with Fusarium-produced mycotoxins, specifically deoxynivalenol (DON). Mycotoxins accumulate in the grain, making it unfit for consumption by humans and animals. Breeding cultivars with high disease resistance and low mycotoxin contamination is a priority for wheat breeders. However, DON measurement in breeding programs is expensive and time consuming due to the lack of efficient quantification methods. In this study, we established a simple fast chromatography-tandem mass spectrometry method, which employed a one-step acetonitrile extraction protocol with a short guard column to reduce complexity, cost, and analysis time. To ensure robustness and reproducibility, the method was validated according to the U.S. Food and Drug Administration Guidance for Bioanalytical Method Validation. Furthermore, the method was applied for determination of DON in 102 wheat grain samples. Obtained results highly correlated with the conventional immunological method for all tested samples. With its ease of use, rapid sample analysis, and high sensitivity and accuracy, the method could be integrated into current FHB breeding programs to increase breeding efficiency and accelerate screening progress to identify germplasm with increased resistance to DON accumulation. [Figure: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-03-22-0024-TAdeoxynivalenol (DON)fast chromatographyFusarium head blightmycotoxintandem mass spectrometrywheat |
| spellingShingle | Lipu Wang Deborah Michel Wentao Zhang Anas El-Aneed Pierre R. Fobert Yuefeng Ruan Samia Berraies Richard Cuthbert Hadley R. Kutcher A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain PhytoFrontiers deoxynivalenol (DON) fast chromatography Fusarium head blight mycotoxin tandem mass spectrometry wheat |
| title | A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain |
| title_full | A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain |
| title_fullStr | A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain |
| title_full_unstemmed | A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain |
| title_short | A High-Throughput Fast Chromatography-Tandem Mass Spectrometry-Based Method for Deoxynivalenol Quantification in Wheat Grain |
| title_sort | high throughput fast chromatography tandem mass spectrometry based method for deoxynivalenol quantification in wheat grain |
| topic | deoxynivalenol (DON) fast chromatography Fusarium head blight mycotoxin tandem mass spectrometry wheat |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-03-22-0024-TA |
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