Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea
ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Fruticultura
2018-02-01
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| Series: | Revista Brasileira de Fruticultura |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802&lng=en&tlng=en |
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| author | NAYARA NIELE SACOMAN ALEXANDRE PIO VIANA VIRGINIA SILVA CARVALHO EILEEN AZEVEDO SANTOS ROSANA RODRIGUES |
| author_facet | NAYARA NIELE SACOMAN ALEXANDRE PIO VIANA VIRGINIA SILVA CARVALHO EILEEN AZEVEDO SANTOS ROSANA RODRIGUES |
| author_sort | NAYARA NIELE SACOMAN |
| collection | DOAJ |
| description | ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs. |
| first_indexed | 2024-04-12T00:14:00Z |
| format | Article |
| id | doaj.art-8f5b27591f634ccf9c9936353eaaaf53 |
| institution | Directory Open Access Journal |
| issn | 1806-9967 |
| language | English |
| last_indexed | 2024-04-12T00:14:00Z |
| publishDate | 2018-02-01 |
| publisher | Sociedade Brasileira de Fruticultura |
| record_format | Article |
| series | Revista Brasileira de Fruticultura |
| spelling | doaj.art-8f5b27591f634ccf9c9936353eaaaf532022-12-22T03:55:53ZengSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura1806-99672018-02-0140110.1590/0100-29452017607S0100-29452018000100802Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setaceaNAYARA NIELE SACOMANALEXANDRE PIO VIANAVIRGINIA SILVA CARVALHOEILEEN AZEVEDO SANTOSROSANA RODRIGUESABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802&lng=en&tlng=enPTA-ELISAvirose do endurecimento dos frutosmaracujazeiroresistência de plantas |
| spellingShingle | NAYARA NIELE SACOMAN ALEXANDRE PIO VIANA VIRGINIA SILVA CARVALHO EILEEN AZEVEDO SANTOS ROSANA RODRIGUES Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea Revista Brasileira de Fruticultura PTA-ELISA virose do endurecimento dos frutos maracujazeiro resistência de plantas |
| title | Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
| title_full | Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
| title_fullStr | Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
| title_full_unstemmed | Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
| title_short | Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea |
| title_sort | resistance to cowpea aphid borne mosaic virus in in vitro germinated genotypes of passiflora setacea |
| topic | PTA-ELISA virose do endurecimento dos frutos maracujazeiro resistência de plantas |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100802&lng=en&tlng=en |
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