Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures
<p>Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced <em>Ank</e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2014-07-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2415 |
| _version_ | 1811322237407461376 |
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| author | H.G. Xu W. Zhang Q. Zheng Y.F. Yu L.F. Deng H. Wang P. Liu M. Zhang |
| author_facet | H.G. Xu W. Zhang Q. Zheng Y.F. Yu L.F. Deng H. Wang P. Liu M. Zhang |
| author_sort | H.G. Xu |
| collection | DOAJ |
| description | <p>Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced <em>Ank</em> gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcification of endplate chondrocytes by decrease the expression of <em>Ank</em> gene. In this study, we investigated the relation of intermittent cyclic mechanical unconfined compression (ICMC) and <em>Ank</em> gene expression. We found that ICMC decreased the <em>Ank</em> gene expression in the endplate chondrocytes, and there was an decreased in the TGF-β1 expression after ICMC stimulation. The <em>Ank</em> gene expression significantly increased when treated by transforming growth factor alpha 1 (TGF-β1) in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our results implicate that ICMC-induced downregulation of <em>Ank</em> gene expression may be regulated by TGF-β1 in endplate chondrocytes.<strong></strong></p> |
| first_indexed | 2024-04-13T13:31:38Z |
| format | Article |
| id | doaj.art-8f5f6a59baab4f5397ff9e208fc396eb |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-13T13:31:38Z |
| publishDate | 2014-07-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-8f5f6a59baab4f5397ff9e208fc396eb2022-12-22T02:44:56ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062014-07-0158310.4081/ejh.2014.24151408Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional culturesH.G. Xu0W. Zhang1Q. Zheng2Y.F. Yu3L.F. Deng4H. Wang5P. Liu6M. Zhang7Wannan Medical CollegeWannan Medical CollegeWannan Medical CollegeInstitute of Health SciencesInstitute of Health SciencesWannan Medical CollegeWannan Medical CollegeWannan Medical College<p>Mechanical stimulation is known to regulate the calcification of endplate chondrocytes. The ANK protein has a strong influence on anti-calcification by transports intracellular inorganic pyrophosphate (PPi) to the extracellular. It is known that TGF-β1 is able to induced <em>Ank</em> gene expression and protect chondrocyte calcification. Intermittent cyclic mechanical tension (ICMT) could induce calcification of endplate chondrocytes by decrease the expression of <em>Ank</em> gene. In this study, we investigated the relation of intermittent cyclic mechanical unconfined compression (ICMC) and <em>Ank</em> gene expression. We found that ICMC decreased the <em>Ank</em> gene expression in the endplate chondrocytes, and there was an decreased in the TGF-β1 expression after ICMC stimulation. The <em>Ank</em> gene expression significantly increased when treated by transforming growth factor alpha 1 (TGF-β1) in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our results implicate that ICMC-induced downregulation of <em>Ank</em> gene expression may be regulated by TGF-β1 in endplate chondrocytes.<strong></strong></p>http://www.ejh.it/index.php/ejh/article/view/2415Endplate chondrocytesICMCAnkTGF-β. |
| spellingShingle | H.G. Xu W. Zhang Q. Zheng Y.F. Yu L.F. Deng H. Wang P. Liu M. Zhang Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures European Journal of Histochemistry Endplate chondrocytes ICMC Ank TGF-β. |
| title | Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures |
| title_full | Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures |
| title_fullStr | Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures |
| title_full_unstemmed | Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures |
| title_short | Investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three-dimensional cultures |
| title_sort | investigating conversion of endplate chondrocytes induced by intermittent cyclic mechanical unconfined compression in three dimensional cultures |
| topic | Endplate chondrocytes ICMC Ank TGF-β. |
| url | http://www.ejh.it/index.php/ejh/article/view/2415 |
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