Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells
Methylene blue (MB) is a widely used dye and photodynamic therapy (PDT) agent that can produce reactive oxygen species (ROS) after light exposure, triggering apoptosis. However, it is hard for the dye to penetrate through the cell membrane, leading to poor cellular uptake; thus, drug carriers, which...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/9/1/14 |
| _version_ | 1818067785287204864 |
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| author | Po-Ting Wu Chih-Ling Lin Che-Wei Lin Ning-Chu Chang Wei-Bor Tsai Jiashing Yu |
| author_facet | Po-Ting Wu Chih-Ling Lin Che-Wei Lin Ning-Chu Chang Wei-Bor Tsai Jiashing Yu |
| author_sort | Po-Ting Wu |
| collection | DOAJ |
| description | Methylene blue (MB) is a widely used dye and photodynamic therapy (PDT) agent that can produce reactive oxygen species (ROS) after light exposure, triggering apoptosis. However, it is hard for the dye to penetrate through the cell membrane, leading to poor cellular uptake; thus, drug carriers, which could enhance the cellular uptake, are a suitable solution. In addition, the defective vessels resulting from fast vessel outgrowth leads to an enhanced permeability and retention (EPR) effect, which gives nanoscale drug carriers a promising potential. In this study, we applied poly(12-(methacryloyloxy)dodecyl phosphorylcholine), a zwitterionic polymer-lipid, to self-assemble into liposomes and encapsulate MB (MB-liposome). Its properties of high stability and fast intracellular uptake were confirmed, and the higher in vitro ROS generation ability of MB-liposomes than that of free MB was also verified. For in vivo tests, we examined the toxicity in mice via tail vein injection. With the features found, MB-liposome has the potential of being an effective PDT nano agent for cancer therapy. |
| first_indexed | 2024-12-10T15:29:12Z |
| format | Article |
| id | doaj.art-8f5fe10b21024eb4b47b932e038a1f6c |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-10T15:29:12Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-8f5fe10b21024eb4b47b932e038a1f6c2022-12-22T01:43:26ZengMDPI AGNanomaterials2079-49912018-12-01911410.3390/nano9010014nano9010014Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer CellsPo-Ting Wu0Chih-Ling Lin1Che-Wei Lin2Ning-Chu Chang3Wei-Bor Tsai4Jiashing Yu5Department of Chemical Engineering, National Taiwan University, Taipei 103, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 103, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 103, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 103, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 103, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 103, TaiwanMethylene blue (MB) is a widely used dye and photodynamic therapy (PDT) agent that can produce reactive oxygen species (ROS) after light exposure, triggering apoptosis. However, it is hard for the dye to penetrate through the cell membrane, leading to poor cellular uptake; thus, drug carriers, which could enhance the cellular uptake, are a suitable solution. In addition, the defective vessels resulting from fast vessel outgrowth leads to an enhanced permeability and retention (EPR) effect, which gives nanoscale drug carriers a promising potential. In this study, we applied poly(12-(methacryloyloxy)dodecyl phosphorylcholine), a zwitterionic polymer-lipid, to self-assemble into liposomes and encapsulate MB (MB-liposome). Its properties of high stability and fast intracellular uptake were confirmed, and the higher in vitro ROS generation ability of MB-liposomes than that of free MB was also verified. For in vivo tests, we examined the toxicity in mice via tail vein injection. With the features found, MB-liposome has the potential of being an effective PDT nano agent for cancer therapy.http://www.mdpi.com/2079-4991/9/1/14liposomemethylene bluebreast cancer cellphotodynamic therapyzwitterion |
| spellingShingle | Po-Ting Wu Chih-Ling Lin Che-Wei Lin Ning-Chu Chang Wei-Bor Tsai Jiashing Yu Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells Nanomaterials liposome methylene blue breast cancer cell photodynamic therapy zwitterion |
| title | Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells |
| title_full | Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells |
| title_fullStr | Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells |
| title_full_unstemmed | Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells |
| title_short | Methylene-Blue-Encapsulated Liposomes as Photodynamic Therapy Nano Agents for Breast Cancer Cells |
| title_sort | methylene blue encapsulated liposomes as photodynamic therapy nano agents for breast cancer cells |
| topic | liposome methylene blue breast cancer cell photodynamic therapy zwitterion |
| url | http://www.mdpi.com/2079-4991/9/1/14 |
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