Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma
Dynamic fluorescence images were obtained from xenografts bearing a subcutaneous human Kaposi's sarcoma (KS1767) immediately following the intravenous injection of an integrin-receptor targeting Cy5.5-c(KRGDf) at a dose ranging from 0.75 to 6 nmol/mouse. The fluorescence images were acquired us...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200505103 |
| _version_ | 1826904033491681280 |
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| author | Sunkuk Kwon Shi Ke Jessica P. Houston Wei Wang Qingping Wu Chun Li Eva M. Sevick-Muraca |
| author_facet | Sunkuk Kwon Shi Ke Jessica P. Houston Wei Wang Qingping Wu Chun Li Eva M. Sevick-Muraca |
| author_sort | Sunkuk Kwon |
| collection | DOAJ |
| description | Dynamic fluorescence images were obtained from xenografts bearing a subcutaneous human Kaposi's sarcoma (KS1767) immediately following the intravenous injection of an integrin-receptor targeting Cy5.5-c(KRGDf) at a dose ranging from 0.75 to 6 nmol/mouse. The fluorescence images were acquired using an intensified charge-coupled device system and were analyzed with a three-compartment pharmacokinetic (PK) model to determine uptake parameters in the tumor and normal tissue regions of interest as a function of administered dose. Our results show that the uptake of Cy5.5-c(KRGDf) in tumor regions were: (i) significantly greater than the contralateral normal tissue regions; (ii) linearly increased with dose of Cy5.5-c(KRGDf) up to 1.5 nmol/mouse; and (iii) blocked by preinjection of c(KRGDf). Above doses of 1.5 nmol/mouse, the uptake no longer increased with dose, suggesting integrin receptor saturation. In normal tissues, the PK uptake parameters were not influenced by Cy5.5-c(KRGDf) dose nor by the preadministration of c(KRGDf). |
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| format | Article |
| id | doaj.art-8f6268496f894dc38f40eedc47c40060 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2025-02-17T08:05:47Z |
| publishDate | 2005-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-8f6268496f894dc38f40eedc47c400602025-01-03T00:12:14ZengSAGE PublishingMolecular Imaging1536-01212005-04-01410.1162/1535350020050510310.1162_15353500200505103Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's SarcomaSunkuk Kwon0Shi Ke1Jessica P. Houston2Wei Wang3Qingping Wu4Chun Li5Eva M. Sevick-MuracaTexas A&M UniversityTexas A&M UniversityTexas A&M UniversityUniversity of Texas M.D. Anderson Cancer CenterUniversity of Texas M.D. Anderson Cancer CenterUniversity of Texas M.D. Anderson Cancer CenterDynamic fluorescence images were obtained from xenografts bearing a subcutaneous human Kaposi's sarcoma (KS1767) immediately following the intravenous injection of an integrin-receptor targeting Cy5.5-c(KRGDf) at a dose ranging from 0.75 to 6 nmol/mouse. The fluorescence images were acquired using an intensified charge-coupled device system and were analyzed with a three-compartment pharmacokinetic (PK) model to determine uptake parameters in the tumor and normal tissue regions of interest as a function of administered dose. Our results show that the uptake of Cy5.5-c(KRGDf) in tumor regions were: (i) significantly greater than the contralateral normal tissue regions; (ii) linearly increased with dose of Cy5.5-c(KRGDf) up to 1.5 nmol/mouse; and (iii) blocked by preinjection of c(KRGDf). Above doses of 1.5 nmol/mouse, the uptake no longer increased with dose, suggesting integrin receptor saturation. In normal tissues, the PK uptake parameters were not influenced by Cy5.5-c(KRGDf) dose nor by the preadministration of c(KRGDf).https://doi.org/10.1162/15353500200505103 |
| spellingShingle | Sunkuk Kwon Shi Ke Jessica P. Houston Wei Wang Qingping Wu Chun Li Eva M. Sevick-Muraca Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma Molecular Imaging |
| title | Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma |
| title_full | Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma |
| title_fullStr | Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma |
| title_full_unstemmed | Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma |
| title_short | Imaging Dose-dependent Pharmacokinetics of an RGD-Fluorescent Dye Conjugate Targeted to αβ Receptor Expressed in Kaposi's Sarcoma |
| title_sort | imaging dose dependent pharmacokinetics of an rgd fluorescent dye conjugate targeted to αβ receptor expressed in kaposi s sarcoma |
| url | https://doi.org/10.1162/15353500200505103 |
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