Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques
Radiographic contrast studies have been recommended to identify the thoracic duct (TD) and its branches before and after surgery for total occlusion. The macroscopic identification of the TD and its branches during surgery usually involves injection of methylene blue (MB). Radiographic contrast and...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2014-06-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201406-0002_visualisation-of-the-thoracic-duct-after-popliteal-lymph-node-injection-in-the-pig-comparison-of-radiographic.php |
| _version_ | 1797897594923384832 |
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| author | D. Vnuk A. Gudan Kurilj D. Maticic G. Dupre |
| author_facet | D. Vnuk A. Gudan Kurilj D. Maticic G. Dupre |
| author_sort | D. Vnuk |
| collection | DOAJ |
| description | Radiographic contrast studies have been recommended to identify the thoracic duct (TD) and its branches before and after surgery for total occlusion. The macroscopic identification of the TD and its branches during surgery usually involves injection of methylene blue (MB). Radiographic contrast and methylene blue can be injected into different anatomical structures (lymph node, lymph vessel, s.c.). The purpose of this study was to compare two different techniques (radiographic and thoracoscopic) for visualisation of the TD after intrapopliteal lymph node injection in the pig. Six piglets from the same litter (two males and four females), two months of age were used. Iohexol at 245 mg/ml was injected into the left popliteal lymph node (LN) under general anaesthesia; hindlimb, abdominal, and thoracic radiographs were taken. A 0.25% methylene blue solution was injected into the right popliteal lymph node and the thoracic duct colouration was assessed thoracoscopically. The thoracic duct was visualised radiographically in one out of six pigs after iohexol injection and thoracoscopically in five out of six pigs after methylene blue injection. The difference was statistically significant (P = 0.040). Popliteal LN lymphangiography using iohexol at 245 mg/ml in piglets should not be the recommended method for TD visualisation. Intrapopliteal injection of a 0.25% solution of methylene blue is recommended as a method of TD visualisation prior to thoracic duct ligation. |
| first_indexed | 2024-04-10T07:59:49Z |
| format | Article |
| id | doaj.art-8f6367fbb73742d7b46224c41f7cf8db |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:49Z |
| publishDate | 2014-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-8f6367fbb73742d7b46224c41f7cf8db2023-02-23T03:50:16ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922014-06-0159627628210.17221/7571-VETMEDvet-201406-0002Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniquesD. Vnuk0A. Gudan Kurilj1D. Maticic2G. Dupre3Faculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Zagreb, CroatiaClinic for Small Animals, University of Veterinary Medicine, Vienna, AustriaRadiographic contrast studies have been recommended to identify the thoracic duct (TD) and its branches before and after surgery for total occlusion. The macroscopic identification of the TD and its branches during surgery usually involves injection of methylene blue (MB). Radiographic contrast and methylene blue can be injected into different anatomical structures (lymph node, lymph vessel, s.c.). The purpose of this study was to compare two different techniques (radiographic and thoracoscopic) for visualisation of the TD after intrapopliteal lymph node injection in the pig. Six piglets from the same litter (two males and four females), two months of age were used. Iohexol at 245 mg/ml was injected into the left popliteal lymph node (LN) under general anaesthesia; hindlimb, abdominal, and thoracic radiographs were taken. A 0.25% methylene blue solution was injected into the right popliteal lymph node and the thoracic duct colouration was assessed thoracoscopically. The thoracic duct was visualised radiographically in one out of six pigs after iohexol injection and thoracoscopically in five out of six pigs after methylene blue injection. The difference was statistically significant (P = 0.040). Popliteal LN lymphangiography using iohexol at 245 mg/ml in piglets should not be the recommended method for TD visualisation. Intrapopliteal injection of a 0.25% solution of methylene blue is recommended as a method of TD visualisation prior to thoracic duct ligation.https://vetmed.agriculturejournals.cz/artkey/vet-201406-0002_visualisation-of-the-thoracic-duct-after-popliteal-lymph-node-injection-in-the-pig-comparison-of-radiographic.phpiohexolmethylene bluepigpopliteal lymph nodethoracic duct |
| spellingShingle | D. Vnuk A. Gudan Kurilj D. Maticic G. Dupre Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques Veterinární Medicína iohexol methylene blue pig popliteal lymph node thoracic duct |
| title | Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques |
| title_full | Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques |
| title_fullStr | Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques |
| title_full_unstemmed | Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques |
| title_short | Visualisation of the thoracic duct after popliteal lymph node injection in the pig: comparison of radiographic and thoracoscopic techniques |
| title_sort | visualisation of the thoracic duct after popliteal lymph node injection in the pig comparison of radiographic and thoracoscopic techniques |
| topic | iohexol methylene blue pig popliteal lymph node thoracic duct |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201406-0002_visualisation-of-the-thoracic-duct-after-popliteal-lymph-node-injection-in-the-pig-comparison-of-radiographic.php |
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