An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19
Introduction: Coronavirus disease 2019 (COVID-19) pandemic continues to be a global public health problem. The present study aimed to investigate SARS-CoV-2 antibodies and evaluate the results using an immunochromatographic method and an ELISA method, in patients with suspected COVID-19, who were te...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2022-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-02/202-208%20Serife%20Yilmaz.pdf |
| _version_ | 1797924051281248256 |
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| author | Şerife YILMAZ Demet HACISEYİTOĞLU Füsun CÖMERT |
| author_facet | Şerife YILMAZ Demet HACISEYİTOĞLU Füsun CÖMERT |
| author_sort | Şerife YILMAZ |
| collection | DOAJ |
| description | Introduction: Coronavirus disease 2019 (COVID-19) pandemic continues to be a global public health problem. The present study aimed to investigate SARS-CoV-2 antibodies and evaluate the results using an immunochromatographic method and an ELISA method, in patients with suspected COVID-19, who were tested by reverse transcription polymerase chain reaction (RT-PCR) technique to detect SARS-CoV-2 RNA.
Materials and Methods: The study was conducted at the SARS-CoV-2 authorized diagnostic laboratory of our hospital at the beginning of the COVID-19 pandemic, between 27 March and 14 May 2020. The nasopharynx and oropharynx swab samples of the patients included in the study were tested by the SARS-CoV-2 RT-PCR (Bioeksen, İstanbul, Turkey), where the serum samples were tested by an immunochromatographic test (Beijing Hotgen Biotech Co., Ltd, China) and Anti-SARS-CoV-2 ELISA IgG (Euroimmun, Luebeck, Germany) test. The dates the patients’ symptoms started and the samples were collected for RT-PCR and serological testing were recorded. The patients were divided into two groups based on the data retrieved from the files containing the clinical information and microbiological results of the patients: individuals, who were diagnosed with COVID-19, and individuals, for whom the COVID-19 diagnosis was excluded.
Results: Of the 115 patients included in the study, 92 (80%) individuals presented to the clinic with COVID-19 similar symptoms, while 23 (20%) individuals had contact with patients diagnosed with COVID-19. Sixty-one (66.3%) of 92 patients with symptoms were diagnosed with COVID-19, where 6 (26%) individuals out of 23 without symptoms were diagnosed with COVID-19. Fifty-one (76.1%) individuals out of 67 patients diagnosed with COVID-19 had positive RT-PCR results. Sixteen individuals with COVID-19, who were tested negative for RT-PCR, had positive Euroimmun Anti-SARS-CoV-2 ELISA IgG results. Of the 67 patients diagnosed with COVID-19, 61 (91%) were symptomatic and the extended time periods between the onset of the symptoms and the date of sampling for the RT-PCR test was associated with lower rates of being tested positive for the RT-PCR test (p= 0.01).
Conclusion: The present study investigated the performance of the COVID-19 tests and showed that antibody tests intended for patients with suspected COVID-19, even in cases where the RT-PCR test is negative, would contribute to the diagnosis. The study contributes in the relevant literature as to which tests would be more useful in certain conditions, considering the fact that there is no test available that can provide 100% accurate results for the diagnosis of COVID-19. |
| first_indexed | 2024-04-10T14:55:37Z |
| format | Article |
| id | doaj.art-8f65133eb2ba4c9cbab9c3a4cb778b02 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1308-5115 |
| language | English |
| last_indexed | 2024-04-10T14:55:37Z |
| publishDate | 2022-06-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-8f65133eb2ba4c9cbab9c3a4cb778b022023-02-15T16:07:23ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1308-51152022-06-0127220220810.5578/flora.20229803An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19Şerife YILMAZ0https://orcid.org/0000-0001-5310-3933Demet HACISEYİTOĞLU1https://orcid.org/0000-0001-7404-8347Füsun CÖMERT2https://orcid.org/0000-0003-0161-6897Department of Medical Microbiology, Bülent Ecevit University Faculty of Medicine, Zonguldak, TürkiyeDepartment of Medical Microbiology, Bülent Ecevit University Faculty of Medicine, Zonguldak, TürkiyeDepartment of Medical Microbiology, Bülent Ecevit University Faculty of Medicine, Zonguldak, TürkiyeIntroduction: Coronavirus disease 2019 (COVID-19) pandemic continues to be a global public health problem. The present study aimed to investigate SARS-CoV-2 antibodies and evaluate the results using an immunochromatographic method and an ELISA method, in patients with suspected COVID-19, who were tested by reverse transcription polymerase chain reaction (RT-PCR) technique to detect SARS-CoV-2 RNA. Materials and Methods: The study was conducted at the SARS-CoV-2 authorized diagnostic laboratory of our hospital at the beginning of the COVID-19 pandemic, between 27 March and 14 May 2020. The nasopharynx and oropharynx swab samples of the patients included in the study were tested by the SARS-CoV-2 RT-PCR (Bioeksen, İstanbul, Turkey), where the serum samples were tested by an immunochromatographic test (Beijing Hotgen Biotech Co., Ltd, China) and Anti-SARS-CoV-2 ELISA IgG (Euroimmun, Luebeck, Germany) test. The dates the patients’ symptoms started and the samples were collected for RT-PCR and serological testing were recorded. The patients were divided into two groups based on the data retrieved from the files containing the clinical information and microbiological results of the patients: individuals, who were diagnosed with COVID-19, and individuals, for whom the COVID-19 diagnosis was excluded. Results: Of the 115 patients included in the study, 92 (80%) individuals presented to the clinic with COVID-19 similar symptoms, while 23 (20%) individuals had contact with patients diagnosed with COVID-19. Sixty-one (66.3%) of 92 patients with symptoms were diagnosed with COVID-19, where 6 (26%) individuals out of 23 without symptoms were diagnosed with COVID-19. Fifty-one (76.1%) individuals out of 67 patients diagnosed with COVID-19 had positive RT-PCR results. Sixteen individuals with COVID-19, who were tested negative for RT-PCR, had positive Euroimmun Anti-SARS-CoV-2 ELISA IgG results. Of the 67 patients diagnosed with COVID-19, 61 (91%) were symptomatic and the extended time periods between the onset of the symptoms and the date of sampling for the RT-PCR test was associated with lower rates of being tested positive for the RT-PCR test (p= 0.01). Conclusion: The present study investigated the performance of the COVID-19 tests and showed that antibody tests intended for patients with suspected COVID-19, even in cases where the RT-PCR test is negative, would contribute to the diagnosis. The study contributes in the relevant literature as to which tests would be more useful in certain conditions, considering the fact that there is no test available that can provide 100% accurate results for the diagnosis of COVID-19.http://floradergisi.org/managete/fu_folder/2022-02/202-208%20Serife%20Yilmaz.pdfcovid-19sars-cov-2rt-pcr |
| spellingShingle | Şerife YILMAZ Demet HACISEYİTOĞLU Füsun CÖMERT An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19 Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi covid-19 sars-cov-2 rt-pcr |
| title | An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19 |
| title_full | An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19 |
| title_fullStr | An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19 |
| title_full_unstemmed | An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19 |
| title_short | An Investigation on the Performance of Different Microbiological Methods Used in the Diagnosis of COVID-19 |
| title_sort | investigation on the performance of different microbiological methods used in the diagnosis of covid 19 |
| topic | covid-19 sars-cov-2 rt-pcr |
| url | http://floradergisi.org/managete/fu_folder/2022-02/202-208%20Serife%20Yilmaz.pdf |
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