Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers
Landraces are valuable sources of genetic characteristics, which are of plant breeders’ interest to include in breeding programs for crop improvement. We assess the inter- and intra-genetic variability among 96 accessions representing three Hail tomato landrace using DNA-based marker sequence-relate...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/13/3/135 |
| _version_ | 1797540501273968640 |
|---|---|
| author | Reem H. Alzahib Hussein M. Migdadi Abdullah A. Al Ghamdi Mona S. Alwahibi Muhammad Afzal Ehab H. Elharty Salem S. Alghamdi |
| author_facet | Reem H. Alzahib Hussein M. Migdadi Abdullah A. Al Ghamdi Mona S. Alwahibi Muhammad Afzal Ehab H. Elharty Salem S. Alghamdi |
| author_sort | Reem H. Alzahib |
| collection | DOAJ |
| description | Landraces are valuable sources of genetic characteristics, which are of plant breeders’ interest to include in breeding programs for crop improvement. We assess the inter- and intra-genetic variability among 96 accessions representing three Hail tomato landrace using DNA-based marker sequence-related amplified polymorphism (SRAP). Seven SRAP primer combinations generated 55 alleles with a polymorphism of 100%, and an average of 7.86 polymorphic alleles per pair of primers. All primers showed an average of 0.68 polymorphic information content (PIC) value and discrimination power (DP) of 14.29. Principal coordinate analysis (PCoA) confirmed the clustering produced via the UPGMA similarity dendrogram allowed for the grouping of the 96 accessions according to its gene bank accession numbers and showed relatively good separation between landraces. A similarity value ranged from 0.04 to 1.0 among accessions of Hail 747, 0.05 to 1.0 in Hail 1072, and from 0.16 to 0.92 in Hail 548. These results showed the landraces harbor a wide range of genetic diversity at both inter and intra-variation levels. AMOVA showed that most of the genetic variation was because of differences within populations (87%). Tomato Hail landraces have well-differentiated genetic populations and admixtures, where Hail 747 formed their separate group, and both Hail 548 and Hail 1072 were admixed, and some accessions showed more diversity pattern. We have to take the SRAP technique’s effectiveness in the study of genetic variability among and within landraces into consideration in the tomato-breeding programs through marker-assessed selection. |
| first_indexed | 2024-03-10T13:01:39Z |
| format | Article |
| id | doaj.art-8f6936fef32f4df583a5392ca55d1217 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T13:01:39Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-8f6936fef32f4df583a5392ca55d12172023-11-21T11:27:35ZengMDPI AGDiversity1424-28182021-03-0113313510.3390/d13030135Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism MarkersReem H. Alzahib0Hussein M. Migdadi1Abdullah A. Al Ghamdi2Mona S. Alwahibi3Muhammad Afzal4Ehab H. Elharty5Salem S. Alghamdi6Department of Botany & Microbiology, College of Sciences, King Saud University, Riyadh 11451, Saudi ArabiaCollege of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany & Microbiology, College of Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany & Microbiology, College of Sciences, King Saud University, Riyadh 11451, Saudi ArabiaCollege of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaCollege of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaCollege of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaLandraces are valuable sources of genetic characteristics, which are of plant breeders’ interest to include in breeding programs for crop improvement. We assess the inter- and intra-genetic variability among 96 accessions representing three Hail tomato landrace using DNA-based marker sequence-related amplified polymorphism (SRAP). Seven SRAP primer combinations generated 55 alleles with a polymorphism of 100%, and an average of 7.86 polymorphic alleles per pair of primers. All primers showed an average of 0.68 polymorphic information content (PIC) value and discrimination power (DP) of 14.29. Principal coordinate analysis (PCoA) confirmed the clustering produced via the UPGMA similarity dendrogram allowed for the grouping of the 96 accessions according to its gene bank accession numbers and showed relatively good separation between landraces. A similarity value ranged from 0.04 to 1.0 among accessions of Hail 747, 0.05 to 1.0 in Hail 1072, and from 0.16 to 0.92 in Hail 548. These results showed the landraces harbor a wide range of genetic diversity at both inter and intra-variation levels. AMOVA showed that most of the genetic variation was because of differences within populations (87%). Tomato Hail landraces have well-differentiated genetic populations and admixtures, where Hail 747 formed their separate group, and both Hail 548 and Hail 1072 were admixed, and some accessions showed more diversity pattern. We have to take the SRAP technique’s effectiveness in the study of genetic variability among and within landraces into consideration in the tomato-breeding programs through marker-assessed selection.https://www.mdpi.com/1424-2818/13/3/135landraces<i>Solanum lycopersicum</i>SRAPAMOVA |
| spellingShingle | Reem H. Alzahib Hussein M. Migdadi Abdullah A. Al Ghamdi Mona S. Alwahibi Muhammad Afzal Ehab H. Elharty Salem S. Alghamdi Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers Diversity landraces <i>Solanum lycopersicum</i> SRAP AMOVA |
| title | Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers |
| title_full | Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers |
| title_fullStr | Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers |
| title_full_unstemmed | Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers |
| title_short | Exploring Genetic Variability among and within Hail Tomato Landraces Based on Sequence-Related Amplified Polymorphism Markers |
| title_sort | exploring genetic variability among and within hail tomato landraces based on sequence related amplified polymorphism markers |
| topic | landraces <i>Solanum lycopersicum</i> SRAP AMOVA |
| url | https://www.mdpi.com/1424-2818/13/3/135 |
| work_keys_str_mv | AT reemhalzahib exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers AT husseinmmigdadi exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers AT abdullahaalghamdi exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers AT monasalwahibi exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers AT muhammadafzal exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers AT ehabhelharty exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers AT salemsalghamdi exploringgeneticvariabilityamongandwithinhailtomatolandracesbasedonsequencerelatedamplifiedpolymorphismmarkers |