Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles
Abstract This study evaluated the median lethal concentration of silver nanoparticles and their effects in fish tambaqui Colossoma macropomum. Therefore, an acute toxicity assay was carried out in completely randomized design evaluating six different concentrations of silver nanoparticles on blood p...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2024-03-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000101306&tlng=en |
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| author | JULIANA O. MENESES FERNANDA DOS SANTOS CUNHA JOEL ARTUR R. DIAS NATALINO DA COSTA SOUSA MÁRCIA VALÉRIA S. DO COUTO ANA FLÁVIA S. DA CUNHA PETERSON EMMANUEL G. PAIXÃO HIGO A. ABE VICTOR RUAN S. NASCIMENTO JULIANA C. CARDOSO LUIZ P. DA COSTA RODRIGO Y. FUJIMOTO |
| author_facet | JULIANA O. MENESES FERNANDA DOS SANTOS CUNHA JOEL ARTUR R. DIAS NATALINO DA COSTA SOUSA MÁRCIA VALÉRIA S. DO COUTO ANA FLÁVIA S. DA CUNHA PETERSON EMMANUEL G. PAIXÃO HIGO A. ABE VICTOR RUAN S. NASCIMENTO JULIANA C. CARDOSO LUIZ P. DA COSTA RODRIGO Y. FUJIMOTO |
| author_sort | JULIANA O. MENESES |
| collection | DOAJ |
| description | Abstract This study evaluated the median lethal concentration of silver nanoparticles and their effects in fish tambaqui Colossoma macropomum. Therefore, an acute toxicity assay was carried out in completely randomized design evaluating six different concentrations of silver nanoparticles on blood parameters of tambaqui. The silver nanoparticles were produced by chemical reduction with polyvinyl alcohol (AgNP-PVA). The lethal concentration 50% (LC50) was estimated using probit regression. The blood was collected, analyzed and the data were submitted to T-test (dying x surviving fish) and Tukey test (surviving fish). An increase in glucose, hematocrit, total plasma protein, hemoglobin, erythrocytes, leukocytes, monocytes, and neutrophils as well as reduced MCV (mean corpuscular volume) in dying fish compared to surviving fish were observed. Survived fish exposed to 187.5 µg/L showed an increase in hematocrit, MCV, and MCH and a reduction in erythrocytes, total numbers of leukocyte, thrombocyte, lymphocyte, and neutrophil. The fish exposed to concentrations below 125 µg/L, had returned the blood parameter to baselines compared to control. The estimated LC50 was 165.09 µg/L and was classified as highly toxic for the fish tambaqui. In higher concentrations, it causes an acute respiratory toxicity, but in concentrations below 125 µg/L, the fish can adapt to the stressing agent. |
| first_indexed | 2024-03-07T15:42:43Z |
| format | Article |
| id | doaj.art-8f69b95d8fcf4fcfbf5a64fddda2943c |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-03-07T15:42:43Z |
| publishDate | 2024-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-8f69b95d8fcf4fcfbf5a64fddda2943c2024-03-05T07:39:08ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902024-03-0196110.1590/0001-3765202420230159Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticlesJULIANA O. MENESEShttps://orcid.org/0000-0002-8680-512XFERNANDA DOS SANTOS CUNHAhttps://orcid.org/0000-0003-2883-7542JOEL ARTUR R. DIAShttps://orcid.org/0000-0001-9751-0207NATALINO DA COSTA SOUSAhttps://orcid.org/0000-0002-7580-5650MÁRCIA VALÉRIA S. DO COUTOhttps://orcid.org/0000-0001-9235-0996ANA FLÁVIA S. DA CUNHAhttps://orcid.org/0000-0001-9277-3980PETERSON EMMANUEL G. PAIXÃOhttps://orcid.org/0000-0002-8949-4232HIGO A. ABEhttps://orcid.org/0000-0002-5717-9641VICTOR RUAN S. NASCIMENTOhttps://orcid.org/0000-0002-1150-332XJULIANA C. CARDOSOhttps://orcid.org/0000-0002-0357-4144LUIZ P. DA COSTAhttps://orcid.org/0000-0002-2632-6485RODRIGO Y. FUJIMOTOhttps://orcid.org/0000-0002-5039-4445Abstract This study evaluated the median lethal concentration of silver nanoparticles and their effects in fish tambaqui Colossoma macropomum. Therefore, an acute toxicity assay was carried out in completely randomized design evaluating six different concentrations of silver nanoparticles on blood parameters of tambaqui. The silver nanoparticles were produced by chemical reduction with polyvinyl alcohol (AgNP-PVA). The lethal concentration 50% (LC50) was estimated using probit regression. The blood was collected, analyzed and the data were submitted to T-test (dying x surviving fish) and Tukey test (surviving fish). An increase in glucose, hematocrit, total plasma protein, hemoglobin, erythrocytes, leukocytes, monocytes, and neutrophils as well as reduced MCV (mean corpuscular volume) in dying fish compared to surviving fish were observed. Survived fish exposed to 187.5 µg/L showed an increase in hematocrit, MCV, and MCH and a reduction in erythrocytes, total numbers of leukocyte, thrombocyte, lymphocyte, and neutrophil. The fish exposed to concentrations below 125 µg/L, had returned the blood parameter to baselines compared to control. The estimated LC50 was 165.09 µg/L and was classified as highly toxic for the fish tambaqui. In higher concentrations, it causes an acute respiratory toxicity, but in concentrations below 125 µg/L, the fish can adapt to the stressing agent.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000101306&tlng=enAquaculturebiotechnologyhematologynanotechnology |
| spellingShingle | JULIANA O. MENESES FERNANDA DOS SANTOS CUNHA JOEL ARTUR R. DIAS NATALINO DA COSTA SOUSA MÁRCIA VALÉRIA S. DO COUTO ANA FLÁVIA S. DA CUNHA PETERSON EMMANUEL G. PAIXÃO HIGO A. ABE VICTOR RUAN S. NASCIMENTO JULIANA C. CARDOSO LUIZ P. DA COSTA RODRIGO Y. FUJIMOTO Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles Anais da Academia Brasileira de Ciências Aquaculture biotechnology hematology nanotechnology |
| title | Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles |
| title_full | Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles |
| title_fullStr | Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles |
| title_full_unstemmed | Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles |
| title_short | Blood cell alterations in Colossoma macropomum juveniles caused by silver nanoparticles |
| title_sort | blood cell alterations in colossoma macropomum juveniles caused by silver nanoparticles |
| topic | Aquaculture biotechnology hematology nanotechnology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000101306&tlng=en |
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