Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity
Abstract Background Contribution of efflux pumps in development of antimicrobial resistance has been largely addressed in Gram negative and to a much lesser extent in Gram positive bacteria. Measuring accumulation of Hoechst (H) dye is known as a safe and rapid method for monitoring efflux activity...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02595-x |
| _version_ | 1811320641419214848 |
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| author | Mohammad Hossein Sobhanipoor Roya Ahmadrajabi Hossein Hosseini Nave Fereshteh Saffari |
| author_facet | Mohammad Hossein Sobhanipoor Roya Ahmadrajabi Hossein Hosseini Nave Fereshteh Saffari |
| author_sort | Mohammad Hossein Sobhanipoor |
| collection | DOAJ |
| description | Abstract Background Contribution of efflux pumps in development of antimicrobial resistance has been largely addressed in Gram negative and to a much lesser extent in Gram positive bacteria. Measuring accumulation of Hoechst (H) dye is known as a safe and rapid method for monitoring efflux activity in bacteria. Antimicrobial effects of metal nanoparticles have been attributed in part to inhibition of efflux pumps. This study aimed to first determine efflux activity in enterococci by Hoechst accumulation assay, and to second characterize the role of zinc oxide nanoparticles (ZnONPs) in inhibition of these pumps. Results Increased accumulation of Hoechst dye showed more potential of ZnONPs in efflux inhibition compared with CCCP. H33258 represented more suitability for accumulation studies in enterococci. Two to six-fold reduction in minimum inhibitory concentration (MIC) values of antimicrobial agents in the presence of ZnONPs was observed. Conclusions Efflux activity in enterococcal strains can be measured by H33258 accumulation assay. Application of ZnONPs as an efflux inhibitor, may rejuvenate the use of conventional antimicrobial agents against these bacteria. |
| first_indexed | 2024-04-13T13:03:02Z |
| format | Article |
| id | doaj.art-8f6b06a880134d128c7e2888cbbf498a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T13:03:02Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-8f6b06a880134d128c7e2888cbbf498a2022-12-22T02:45:51ZengBMCBMC Microbiology1471-21802022-08-012211910.1186/s12866-022-02595-xDetermination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activityMohammad Hossein Sobhanipoor0Roya Ahmadrajabi1Hossein Hosseini Nave2Fereshteh Saffari3Department of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical SciencesDepartment of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical SciencesDepartment of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical SciencesMedical Mycology and Bacteriology Research Center, Kerman University of Medical SciencesAbstract Background Contribution of efflux pumps in development of antimicrobial resistance has been largely addressed in Gram negative and to a much lesser extent in Gram positive bacteria. Measuring accumulation of Hoechst (H) dye is known as a safe and rapid method for monitoring efflux activity in bacteria. Antimicrobial effects of metal nanoparticles have been attributed in part to inhibition of efflux pumps. This study aimed to first determine efflux activity in enterococci by Hoechst accumulation assay, and to second characterize the role of zinc oxide nanoparticles (ZnONPs) in inhibition of these pumps. Results Increased accumulation of Hoechst dye showed more potential of ZnONPs in efflux inhibition compared with CCCP. H33258 represented more suitability for accumulation studies in enterococci. Two to six-fold reduction in minimum inhibitory concentration (MIC) values of antimicrobial agents in the presence of ZnONPs was observed. Conclusions Efflux activity in enterococcal strains can be measured by H33258 accumulation assay. Application of ZnONPs as an efflux inhibitor, may rejuvenate the use of conventional antimicrobial agents against these bacteria.https://doi.org/10.1186/s12866-022-02595-xEnterococcusEfflux pumpHoechstNanoparticlesZinc oxideH33258 accumulation assay |
| spellingShingle | Mohammad Hossein Sobhanipoor Roya Ahmadrajabi Hossein Hosseini Nave Fereshteh Saffari Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity BMC Microbiology Enterococcus Efflux pump Hoechst Nanoparticles Zinc oxide H33258 accumulation assay |
| title | Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity |
| title_full | Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity |
| title_fullStr | Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity |
| title_full_unstemmed | Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity |
| title_short | Determination of efflux activity in Enterococci by Hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity |
| title_sort | determination of efflux activity in enterococci by hoechst accumulation assay and the role of zinc oxide nanoparticles in inhibition of this activity |
| topic | Enterococcus Efflux pump Hoechst Nanoparticles Zinc oxide H33258 accumulation assay |
| url | https://doi.org/10.1186/s12866-022-02595-x |
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