MicroRNA expression in bone marrow-derived human multipotent Stromal cells
Abstract Background Multipotent stromal cells (MSCs) are being studied in the field of regenerative medicine for their multi-lineage differentiation and immunoregulatory capacity. MicroRNAs (miRNAs) are short non-coding RNAs that are responsible for regulating gene expression by targeting transcript...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-017-3997-7 |
| _version_ | 1818399235727425536 |
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| author | Ian H. Bellayr Abhinav Kumar Raj K. Puri |
| author_facet | Ian H. Bellayr Abhinav Kumar Raj K. Puri |
| author_sort | Ian H. Bellayr |
| collection | DOAJ |
| description | Abstract Background Multipotent stromal cells (MSCs) are being studied in the field of regenerative medicine for their multi-lineage differentiation and immunoregulatory capacity. MicroRNAs (miRNAs) are short non-coding RNAs that are responsible for regulating gene expression by targeting transcripts, which can impact MSC functions such as cellular proliferation, differentiation, migration and cell death. miRNAs are expressed in MSCs; however, the impact of miRNAs on cellular functions and donor variability is not well understood. Eight MSC lines were expanded to passages 3, 5 and 7, and their miRNA expression was evaluated using microarray technology. Results Statistical analyses of our data revealed that 71 miRNAs out of 939 examined were expressed by this set of MSC lines at all passages and the expression of 11 miRNAs were significantly different between passages 3 and 7, while the expression of 7 miRNAs was significantly different between passages 3 and 5. The expression of these identified miRNAs was evaluated using RT-qPCR for both the first set of MSC lines (n = 6) and a second set of MSC lines (n = 7) expanded from passages 4 to 8. By RT-qPCR only 2 miRNAs, miR-638 and miR-572 were upregulated at passage 7 compared to passage 3 in the first set of MSC lines by 1.71 and 1.54 fold, respectively; and upregulated at passage 8 compared to passage 4 in the second set of MSC lines, 1.35 and 1.59 fold, respectively. Conclusions The expression of miR-638 and miR-572 can distinguish MSCs from two different passages of cell culture. These results may be useful in establishing critical quality attributes of MSCs and determining whether changes in these two miRNAs impact cellular functions. |
| first_indexed | 2024-12-14T07:17:28Z |
| format | Article |
| id | doaj.art-8f6bd91f076745ef99795e9c7e562cb7 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T07:17:28Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-8f6bd91f076745ef99795e9c7e562cb72022-12-21T23:11:41ZengBMCBMC Genomics1471-21642017-08-0118111310.1186/s12864-017-3997-7MicroRNA expression in bone marrow-derived human multipotent Stromal cellsIan H. Bellayr0Abhinav Kumar1Raj K. Puri2Tumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics and Evaluation Research, US Food and Drug AdministrationTumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics and Evaluation Research, US Food and Drug AdministrationTumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics and Evaluation Research, US Food and Drug AdministrationAbstract Background Multipotent stromal cells (MSCs) are being studied in the field of regenerative medicine for their multi-lineage differentiation and immunoregulatory capacity. MicroRNAs (miRNAs) are short non-coding RNAs that are responsible for regulating gene expression by targeting transcripts, which can impact MSC functions such as cellular proliferation, differentiation, migration and cell death. miRNAs are expressed in MSCs; however, the impact of miRNAs on cellular functions and donor variability is not well understood. Eight MSC lines were expanded to passages 3, 5 and 7, and their miRNA expression was evaluated using microarray technology. Results Statistical analyses of our data revealed that 71 miRNAs out of 939 examined were expressed by this set of MSC lines at all passages and the expression of 11 miRNAs were significantly different between passages 3 and 7, while the expression of 7 miRNAs was significantly different between passages 3 and 5. The expression of these identified miRNAs was evaluated using RT-qPCR for both the first set of MSC lines (n = 6) and a second set of MSC lines (n = 7) expanded from passages 4 to 8. By RT-qPCR only 2 miRNAs, miR-638 and miR-572 were upregulated at passage 7 compared to passage 3 in the first set of MSC lines by 1.71 and 1.54 fold, respectively; and upregulated at passage 8 compared to passage 4 in the second set of MSC lines, 1.35 and 1.59 fold, respectively. Conclusions The expression of miR-638 and miR-572 can distinguish MSCs from two different passages of cell culture. These results may be useful in establishing critical quality attributes of MSCs and determining whether changes in these two miRNAs impact cellular functions.http://link.springer.com/article/10.1186/s12864-017-3997-7Multipotent Stromal cellsMicroRNA expressionMicroarray |
| spellingShingle | Ian H. Bellayr Abhinav Kumar Raj K. Puri MicroRNA expression in bone marrow-derived human multipotent Stromal cells BMC Genomics Multipotent Stromal cells MicroRNA expression Microarray |
| title | MicroRNA expression in bone marrow-derived human multipotent Stromal cells |
| title_full | MicroRNA expression in bone marrow-derived human multipotent Stromal cells |
| title_fullStr | MicroRNA expression in bone marrow-derived human multipotent Stromal cells |
| title_full_unstemmed | MicroRNA expression in bone marrow-derived human multipotent Stromal cells |
| title_short | MicroRNA expression in bone marrow-derived human multipotent Stromal cells |
| title_sort | microrna expression in bone marrow derived human multipotent stromal cells |
| topic | Multipotent Stromal cells MicroRNA expression Microarray |
| url | http://link.springer.com/article/10.1186/s12864-017-3997-7 |
| work_keys_str_mv | AT ianhbellayr micrornaexpressioninbonemarrowderivedhumanmultipotentstromalcells AT abhinavkumar micrornaexpressioninbonemarrowderivedhumanmultipotentstromalcells AT rajkpuri micrornaexpressioninbonemarrowderivedhumanmultipotentstromalcells |