Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin

Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots...

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Main Authors: M Montazeri M (M.Sc.), M Azadbakht, H Najafi Zarrini, GhA NematZadeh, A Pakdin Parizi, A Davoodi
Format: Article
Language:English
Published: Institue of Medicinal Plants, ACECR 2018-09-01
Series:Journal of Medicinal Plants
Subjects:
Online Access:http://jmp.ir/article-1-2264-en.html
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author M Montazeri M (M.Sc.)
M Azadbakht
H Najafi Zarrini
GhA NematZadeh
A Pakdin Parizi
A Davoodi
author_facet M Montazeri M (M.Sc.)
M Azadbakht
H Najafi Zarrini
GhA NematZadeh
A Pakdin Parizi
A Davoodi
author_sort M Montazeri M (M.Sc.)
collection DOAJ
description Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Finally, the hypericin have been producted by the hairy roots. Methods: First, the seeds have been incubated in the plant media to hairy roots produced. Then, the hairy roots have been dipped in Rhizobium rizogenes suspension for biotransformation of bacterial genes. Morphological and phytochemical features of hairy roots have been determined in order to select the H. perforatum genotypes with higher hypericin contents. The fresh and dry weight of ten lines clones were measured after 30 days. In addition to, methanolic extracts of final hairy roots have been prepared and hypericin has been isolated and assayed by High Performance Liquid Chromatography. Results: According to the observations, one of ten hairy root lines shows 75-fold higher hypericin content (339.27 ppm) compared to non-transformed H. perforatum (4.58 ppm) in the same of body weight of roots. The clone with the most content of hypericin had significant development of biomass of hairy roots and increase the hypericin production. Transformed clones were varied in morphology, growth, and metabolite productivity. Conclusion: The mentioned methods induce the production of hairy root secondary metabolites in high scale to improve the quality and the quantity of pharmaceutical compounds.
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spelling doaj.art-8f6c9e9ee8474e4095e5369bb648745d2022-12-21T21:35:46ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582018-09-0117675567Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of HypericinM Montazeri M (M.Sc.)0M Azadbakht1H Najafi Zarrini2GhA NematZadeh3A Pakdin Parizi4A Davoodi5 1- Department of Genetic & Agricultural Biotechnology, Agricultural Sciences & Natural Resources University, Sari, Iran 2- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran 1- Department of Genetic & Agricultural Biotechnology, Agricultural Sciences & Natural Resources University, Sari, Iran 1- Department of Genetic & Agricultural Biotechnology, Agricultural Sciences & Natural Resources University, Sari, Iran 1- Department of Genetic & Agricultural Biotechnology, Agricultural Sciences & Natural Resources University, Sari, Iran 2- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Finally, the hypericin have been producted by the hairy roots. Methods: First, the seeds have been incubated in the plant media to hairy roots produced. Then, the hairy roots have been dipped in Rhizobium rizogenes suspension for biotransformation of bacterial genes. Morphological and phytochemical features of hairy roots have been determined in order to select the H. perforatum genotypes with higher hypericin contents. The fresh and dry weight of ten lines clones were measured after 30 days. In addition to, methanolic extracts of final hairy roots have been prepared and hypericin has been isolated and assayed by High Performance Liquid Chromatography. Results: According to the observations, one of ten hairy root lines shows 75-fold higher hypericin content (339.27 ppm) compared to non-transformed H. perforatum (4.58 ppm) in the same of body weight of roots. The clone with the most content of hypericin had significant development of biomass of hairy roots and increase the hypericin production. Transformed clones were varied in morphology, growth, and metabolite productivity. Conclusion: The mentioned methods induce the production of hairy root secondary metabolites in high scale to improve the quality and the quantity of pharmaceutical compounds.http://jmp.ir/article-1-2264-en.html<i>hypericum perforatum</i><i>rhizobium rhizogenes</i>hairy roothplchypericin
spellingShingle M Montazeri M (M.Sc.)
M Azadbakht
H Najafi Zarrini
GhA NematZadeh
A Pakdin Parizi
A Davoodi
Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
Journal of Medicinal Plants
<i>hypericum perforatum</i>
<i>rhizobium rhizogenes</i>
hairy root
hplc
hypericin
title Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
title_full Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
title_fullStr Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
title_full_unstemmed Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
title_short Hairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
title_sort hairy root cultures of hypericum perforatum l a promising method for the high scale production of hypericin
topic <i>hypericum perforatum</i>
<i>rhizobium rhizogenes</i>
hairy root
hplc
hypericin
url http://jmp.ir/article-1-2264-en.html
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