Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation
Follicle-stimulating hormone (FSH), an important hypothalamic-pituitary-gonadal axis (HPG) hormone, is secreted by the pituitary gland. This study confirms that FSH is expressed in chicken follicles at different stages, and positive FSHβ mRNA signals were stronger (P<0.05) in granulosa cells than...
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Elsevier
2021-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921636067 |
| _version_ | 1818729118577983488 |
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| author | Yu-lin BI Shu-yan YANG Hai-yan WANG Guo-bin CHANG Guo-hong CHEN |
| author_facet | Yu-lin BI Shu-yan YANG Hai-yan WANG Guo-bin CHANG Guo-hong CHEN |
| author_sort | Yu-lin BI |
| collection | DOAJ |
| description | Follicle-stimulating hormone (FSH), an important hypothalamic-pituitary-gonadal axis (HPG) hormone, is secreted by the pituitary gland. This study confirms that FSH is expressed in chicken follicles at different stages, and positive FSHβ mRNA signals were stronger (P<0.05) in granulosa cells than in oocytes. The 369 bp coding sequence of FSHβ in ovaries is 100% identical to that in the pituitary gland. The experiment in vitro revealed that the ovary possessed FSH secretory capacity. Further, FSHβ mRNA was significantly upregulated (P<0.05) in follicles and significantly higher (P<0.05) than that in the pituitary gland by approximately 2–23 times with the development. The number of granulosa cells decreased significantly (P<0.05) in the cells with siRNA treatment, confirming that the ovarian FSH could promote granulosa cell proliferation. This view was supported by cell cycle analysis and CCND2 and CCNE2 expression. Further research indicated that no difference (P>0.05) was observed between the number of granulosa cells treated with FSHβ siRNA and in exogenous FSH. However, the number of granulosa cells without FSHβ siRNA transfection was significantly higher (P<0.05) for exogenous FSH. This finding suggests that the proliferative effect of exogenous FSH on ovarian granulosa cells depend on endogenous FSH. This study demonstrated that the FSH gene was expressed in chicken follicles and promoted ovarian granulosa cell proliferation, which enriched the theory on HPG axis. |
| first_indexed | 2024-12-17T22:40:48Z |
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| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-17T22:40:48Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-8f6ecdfc93f343409e16d04d0f5e4bac2022-12-21T21:29:56ZengElsevierJournal of Integrative Agriculture2095-31192021-10-01201027492757Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferationYu-lin BI0Shu-yan YANG1Hai-yan WANG2Guo-bin CHANG3Guo-hong CHEN4College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China; BI Yu-lin, Tel: +86-514-87971833Department of Biochemistry, Capital Institute of Pediatrics, Beijing 100020, P.R.ChinaAquacultural College, Rizhao Polytechnic, Rizhao 276826, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence CHANG Guo-bin, Tel: +86-514-87971833College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence CHEN Guo-hong, Tel: +86-514-87971833Follicle-stimulating hormone (FSH), an important hypothalamic-pituitary-gonadal axis (HPG) hormone, is secreted by the pituitary gland. This study confirms that FSH is expressed in chicken follicles at different stages, and positive FSHβ mRNA signals were stronger (P<0.05) in granulosa cells than in oocytes. The 369 bp coding sequence of FSHβ in ovaries is 100% identical to that in the pituitary gland. The experiment in vitro revealed that the ovary possessed FSH secretory capacity. Further, FSHβ mRNA was significantly upregulated (P<0.05) in follicles and significantly higher (P<0.05) than that in the pituitary gland by approximately 2–23 times with the development. The number of granulosa cells decreased significantly (P<0.05) in the cells with siRNA treatment, confirming that the ovarian FSH could promote granulosa cell proliferation. This view was supported by cell cycle analysis and CCND2 and CCNE2 expression. Further research indicated that no difference (P>0.05) was observed between the number of granulosa cells treated with FSHβ siRNA and in exogenous FSH. However, the number of granulosa cells without FSHβ siRNA transfection was significantly higher (P<0.05) for exogenous FSH. This finding suggests that the proliferative effect of exogenous FSH on ovarian granulosa cells depend on endogenous FSH. This study demonstrated that the FSH gene was expressed in chicken follicles and promoted ovarian granulosa cell proliferation, which enriched the theory on HPG axis.http://www.sciencedirect.com/science/article/pii/S2095311921636067FSHexpressionfolliclegranulosa cell proliferationchicken |
| spellingShingle | Yu-lin BI Shu-yan YANG Hai-yan WANG Guo-bin CHANG Guo-hong CHEN Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation Journal of Integrative Agriculture FSH expression follicle granulosa cell proliferation chicken |
| title | Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation |
| title_full | Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation |
| title_fullStr | Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation |
| title_full_unstemmed | Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation |
| title_short | Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation |
| title_sort | follicle stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation |
| topic | FSH expression follicle granulosa cell proliferation chicken |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921636067 |
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