Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae)
Premise of the study: <i>Ilex paraguariensis</i> is a native tree species from Brazil, Argentina, and Paraguay that is used in the production of beverages, medicines, and cosmetics. Primers flanking microsatellites were developed to investigate genetic parameters in the species. Metho...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-03-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1200245 |
| _version_ | 1818138903276683264 |
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| author | Marlei F. Pereira Ana Y. Ciampi Peter W. Inglis Valderês A. Souza Vânia C. R. Azevedo |
| author_facet | Marlei F. Pereira Ana Y. Ciampi Peter W. Inglis Valderês A. Souza Vânia C. R. Azevedo |
| author_sort | Marlei F. Pereira |
| collection | DOAJ |
| description | Premise of the study: <i>Ilex paraguariensis</i> is a native tree species from Brazil, Argentina, and Paraguay that is used in the production of beverages, medicines, and cosmetics. Primers flanking microsatellites were developed to investigate genetic parameters in the species.
Methods and Results: Using microsatellites cloned from an <i>I. paraguariensis</i> shotgun genomic library, 25 pairs of primers were designed and synthesized. Levels of polymorphism were evaluated in 24 individuals from two populations. Twenty loci were polymorphic, and an average of 4.8 and 4.5 alleles per locus were detected in the two populations, respectively. The mean observed heterozygosity was lower than the expected heterozygosity (0.54 vs. 0.60), indicating a departure from Hardy–Weinberg equilibrium and suggesting endogamy in both populations.
Conclusions: The reported set of markers is highly informative and constitutes a powerful tool for the development of genetic characterization studies in <i>I. paraguariensis</i>. |
| first_indexed | 2024-12-11T10:19:35Z |
| format | Article |
| id | doaj.art-8f7079059dde48818fbd477b6e41d717 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-11T10:19:35Z |
| publishDate | 2013-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-8f7079059dde48818fbd477b6e41d7172022-12-22T01:11:30ZengWileyApplications in Plant Sciences2168-04502013-03-0113120024510.3732/apps.1200245Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae)Marlei F. Pereira0Ana Y. Ciampi1Peter W. Inglis2 Valderês A. Souza3Vânia C. R. Azevedo4Instituto Federal de Educação, Ciência e Tecnologia de Goiás, Rua Mucuri, Lote 1-A, Aparecida de Goiânia, 74968-755 Goiás, Goiás, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, W5 Norte, CP 02372, 70770-917 Brasília, Distrito Federal, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, W5 Norte, CP 02372, 70770-917 Brasília, Distrito Federal, BrazilEmbrapa Floresta, Estrada da Ribeira 111, CP 319, 83411-000 Colombo, Paraná, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, W5 Norte, CP 02372, 70770-917 Brasília, Distrito Federal, BrazilPremise of the study: <i>Ilex paraguariensis</i> is a native tree species from Brazil, Argentina, and Paraguay that is used in the production of beverages, medicines, and cosmetics. Primers flanking microsatellites were developed to investigate genetic parameters in the species. Methods and Results: Using microsatellites cloned from an <i>I. paraguariensis</i> shotgun genomic library, 25 pairs of primers were designed and synthesized. Levels of polymorphism were evaluated in 24 individuals from two populations. Twenty loci were polymorphic, and an average of 4.8 and 4.5 alleles per locus were detected in the two populations, respectively. The mean observed heterozygosity was lower than the expected heterozygosity (0.54 vs. 0.60), indicating a departure from Hardy–Weinberg equilibrium and suggesting endogamy in both populations. Conclusions: The reported set of markers is highly informative and constitutes a powerful tool for the development of genetic characterization studies in <i>I. paraguariensis</i>.http://www.bioone.org/doi/full/10.3732/apps.1200245Aquifoliaceaegenetic conservationgenetic diversityIlex paraguariensismicrosatellitesshotgun cloning |
| spellingShingle | Marlei F. Pereira Ana Y. Ciampi Peter W. Inglis Valderês A. Souza Vânia C. R. Azevedo Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae) Applications in Plant Sciences Aquifoliaceae genetic conservation genetic diversity Ilex paraguariensis microsatellites shotgun cloning |
| title | Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae) |
| title_full | Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae) |
| title_fullStr | Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae) |
| title_full_unstemmed | Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae) |
| title_short | Shotgun Sequencing for Microsatellite Identification in <i>Ilex paraguariensis</i> (Aquifoliaceae) |
| title_sort | shotgun sequencing for microsatellite identification in i ilex paraguariensis i aquifoliaceae |
| topic | Aquifoliaceae genetic conservation genetic diversity Ilex paraguariensis microsatellites shotgun cloning |
| url | http://www.bioone.org/doi/full/10.3732/apps.1200245 |
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