Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents
Interspecific hybridization and accompanying backcross between crops and relatives have been recognized as a powerful method to broaden genetic diversity and transfer desirable adaptive traits. Crosses between radish (<i>Raphanus sativus</i>, RR, 2n = 18) and <i>Brassica oleracea&l...
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MDPI AG
2023-05-01
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| author | Jie Yu Shaolin Lei Shiting Fang Niufang Tai Wei Yu Ziwei Yang Lei Gu Hongcheng Wang Xuye Du Bin Zhu Mengxian Cai |
| author_facet | Jie Yu Shaolin Lei Shiting Fang Niufang Tai Wei Yu Ziwei Yang Lei Gu Hongcheng Wang Xuye Du Bin Zhu Mengxian Cai |
| author_sort | Jie Yu |
| collection | DOAJ |
| description | Interspecific hybridization and accompanying backcross between crops and relatives have been recognized as a powerful method to broaden genetic diversity and transfer desirable adaptive traits. Crosses between radish (<i>Raphanus sativus</i>, RR, 2n = 18) and <i>Brassica oleracea</i> (CC, 2n = 18), which formed allotetraploid Raphanobrassica (RRCC, 2n = 36), initiated the construction of resynthetic allopolyploids. However, these progenies from the backcrosses between Raphanobrassica and the two diploid parents have not been well deciphered. Herein, thousands of backcrosses using both Raphanobrassica and the two diploid parents as pollen donors were employed. Several hybrids with expected (2n = 27) and unexpected chromosome numbers (2n = 26 and 2n = 36) were obtained. Fluorescence in situ hybridization (FISH) analysis with R-genome-specific sequences as probes demonstrated that the genome structures of the two expected hybrids were RRC and CCR, and the genome structures of the three unexpected hybrids were RRRC, CCCR, and RRC’ (harbouring an incomplete C genome). The unexpected hybrids with extra R or C genomes showed similar phenotypic characteristics to their expected hybrids. FISH analysis with C-genome-specific sequences as probes demonstrated that the unexpected allotetraploid hybrids exhibited significantly more intergenomic chromosome pairings than the expected hybrids. The expected and unexpected hybrids provide not only novel germplasm resources for the breeding of radish and <i>B. oleracea</i> but also very important genetic material for genome dosage analysis. |
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| spelling | doaj.art-8f714c7fd37c4d37845298059e82d3a82023-11-17T23:32:54ZengMDPI AGPlants2223-77472023-05-01129187510.3390/plants12091875Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid ParentsJie Yu0Shaolin Lei1Shiting Fang2Niufang Tai3Wei Yu4Ziwei Yang5Lei Gu6Hongcheng Wang7Xuye Du8Bin Zhu9Mengxian Cai10School of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaGuizhou Institute of Oil Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550009, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaSchool of Life Sciences, Guizhou Normal University, Guiyang 550025, ChinaInterspecific hybridization and accompanying backcross between crops and relatives have been recognized as a powerful method to broaden genetic diversity and transfer desirable adaptive traits. Crosses between radish (<i>Raphanus sativus</i>, RR, 2n = 18) and <i>Brassica oleracea</i> (CC, 2n = 18), which formed allotetraploid Raphanobrassica (RRCC, 2n = 36), initiated the construction of resynthetic allopolyploids. However, these progenies from the backcrosses between Raphanobrassica and the two diploid parents have not been well deciphered. Herein, thousands of backcrosses using both Raphanobrassica and the two diploid parents as pollen donors were employed. Several hybrids with expected (2n = 27) and unexpected chromosome numbers (2n = 26 and 2n = 36) were obtained. Fluorescence in situ hybridization (FISH) analysis with R-genome-specific sequences as probes demonstrated that the genome structures of the two expected hybrids were RRC and CCR, and the genome structures of the three unexpected hybrids were RRRC, CCCR, and RRC’ (harbouring an incomplete C genome). The unexpected hybrids with extra R or C genomes showed similar phenotypic characteristics to their expected hybrids. FISH analysis with C-genome-specific sequences as probes demonstrated that the unexpected allotetraploid hybrids exhibited significantly more intergenomic chromosome pairings than the expected hybrids. The expected and unexpected hybrids provide not only novel germplasm resources for the breeding of radish and <i>B. oleracea</i> but also very important genetic material for genome dosage analysis.https://www.mdpi.com/2223-7747/12/9/1875<i>Brassica oleracea</i>radishinterspecific hybridizationunexpected hybridschromosome behaviour |
| spellingShingle | Jie Yu Shaolin Lei Shiting Fang Niufang Tai Wei Yu Ziwei Yang Lei Gu Hongcheng Wang Xuye Du Bin Zhu Mengxian Cai Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents Plants <i>Brassica oleracea</i> radish interspecific hybridization unexpected hybrids chromosome behaviour |
| title | Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents |
| title_full | Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents |
| title_fullStr | Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents |
| title_full_unstemmed | Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents |
| title_short | Identification, Characterization, and Cytological Analysis of Several Unexpected Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid Parents |
| title_sort | identification characterization and cytological analysis of several unexpected hybrids derived from reciprocal crosses between raphanobrassica and its diploid parents |
| topic | <i>Brassica oleracea</i> radish interspecific hybridization unexpected hybrids chromosome behaviour |
| url | https://www.mdpi.com/2223-7747/12/9/1875 |
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