DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control
Abstract With the advent of integrated pest management, the conservation of indigenous populations of natural enemies of pest species has become a relevant practice, necessitating the accurate identification of beneficial species and the inspection of evolutionary mechanisms affecting the long‐time...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-09-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.6631 |
| _version_ | 1819067414665494528 |
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| author | Nemanja Gojković Ljubinka Francuski Jasmina Ludoški Vesna Milankov |
| author_facet | Nemanja Gojković Ljubinka Francuski Jasmina Ludoški Vesna Milankov |
| author_sort | Nemanja Gojković |
| collection | DOAJ |
| description | Abstract With the advent of integrated pest management, the conservation of indigenous populations of natural enemies of pest species has become a relevant practice, necessitating the accurate identification of beneficial species and the inspection of evolutionary mechanisms affecting the long‐time persistence of their populations. The long hoverfly, Sphaerophoria scripta, represents one of the most potent aphidophagous control agents due to a worldwide distribution and a favorable constellation of biological traits. Therefore, we assessed five European S. scripta populations by combining molecular (cytochrome c oxidase subunit I‐ COI, internal transcribed spacer 2‐ ITS2, and allozyme loci) and morphological (wing size and shape) characters. COI sequences retrieved in this study were conjointly analyzed with BOLD/GenBank sequences of the other Sphaerophoria species to evaluate whether COI possessed a sufficient diagnostic value as a DNA barcode marker to consistently delimit allospecific individuals. Additionally, the aforementioned characters were used to inspect the population structure of S. scripta in Europe using methods based on individual‐ and population‐based genetic differences, as well as geometric morphometrics of wing traits. The results indicate numerous shared COI haplotypes among different Sphaerophoria species, thus disqualifying this marker from being an adequate barcoding region in this genus. Conversely, the analyses of population structuring revealed high population connectivity across Europe, therefore indicating strong tolerance of S. scripta to environmental heterogeneity. The results imply a multilocus approach as the next step in molecular identification of different Sphaerophoria species, while confirming the status of S. scripta as a powerful biocontrol agent of economically relevant aphid pests. |
| first_indexed | 2024-12-21T16:17:53Z |
| format | Article |
| id | doaj.art-8f71e92a2330442e915198955c5bfef6 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-21T16:17:53Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-8f71e92a2330442e915198955c5bfef62022-12-21T18:57:39ZengWileyEcology and Evolution2045-77582020-09-0110179428944310.1002/ece3.6631DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological controlNemanja Gojković0Ljubinka Francuski1Jasmina Ludoški2Vesna Milankov3Faculty of Sciences Department of Biology and Ecology University of Novi Sad Novi Sad SerbiaFaculty of Sciences Department of Biology and Ecology University of Novi Sad Novi Sad SerbiaFaculty of Sciences Department of Biology and Ecology University of Novi Sad Novi Sad SerbiaFaculty of Sciences Department of Biology and Ecology University of Novi Sad Novi Sad SerbiaAbstract With the advent of integrated pest management, the conservation of indigenous populations of natural enemies of pest species has become a relevant practice, necessitating the accurate identification of beneficial species and the inspection of evolutionary mechanisms affecting the long‐time persistence of their populations. The long hoverfly, Sphaerophoria scripta, represents one of the most potent aphidophagous control agents due to a worldwide distribution and a favorable constellation of biological traits. Therefore, we assessed five European S. scripta populations by combining molecular (cytochrome c oxidase subunit I‐ COI, internal transcribed spacer 2‐ ITS2, and allozyme loci) and morphological (wing size and shape) characters. COI sequences retrieved in this study were conjointly analyzed with BOLD/GenBank sequences of the other Sphaerophoria species to evaluate whether COI possessed a sufficient diagnostic value as a DNA barcode marker to consistently delimit allospecific individuals. Additionally, the aforementioned characters were used to inspect the population structure of S. scripta in Europe using methods based on individual‐ and population‐based genetic differences, as well as geometric morphometrics of wing traits. The results indicate numerous shared COI haplotypes among different Sphaerophoria species, thus disqualifying this marker from being an adequate barcoding region in this genus. Conversely, the analyses of population structuring revealed high population connectivity across Europe, therefore indicating strong tolerance of S. scripta to environmental heterogeneity. The results imply a multilocus approach as the next step in molecular identification of different Sphaerophoria species, while confirming the status of S. scripta as a powerful biocontrol agent of economically relevant aphid pests.https://doi.org/10.1002/ece3.6631integrated pest managementlandscape geneticswing geometric morphometrics |
| spellingShingle | Nemanja Gojković Ljubinka Francuski Jasmina Ludoški Vesna Milankov DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control Ecology and Evolution integrated pest management landscape genetics wing geometric morphometrics |
| title | DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control |
| title_full | DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control |
| title_fullStr | DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control |
| title_full_unstemmed | DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control |
| title_short | DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control |
| title_sort | dna barcode assessment and population structure of aphidophagous hoverfly sphaerophoria scripta implications for conservation biological control |
| topic | integrated pest management landscape genetics wing geometric morphometrics |
| url | https://doi.org/10.1002/ece3.6631 |
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