Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector
In the presence of this study, the core antigen (HBcAg) of hepatitis B virus (HBV) was amplified and cloned into an eucaryotic expression vector. For this aim, HBcAg DNA was amplified with the primers spesific to HBcAg gene of HBV by polymerase chain reaction (PCR). Afterwards, the PCR product of HB...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2003-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2003-8-2-144-147.pdf |
| _version_ | 1797921709483884544 |
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| author | Yasemin BULUT Aykut ÖZDARENDELİ Mehmet Ziya DOYMAZ Zülal AŞÇI TORAMAN |
| author_facet | Yasemin BULUT Aykut ÖZDARENDELİ Mehmet Ziya DOYMAZ Zülal AŞÇI TORAMAN |
| author_sort | Yasemin BULUT |
| collection | DOAJ |
| description | In the presence of this study, the core antigen (HBcAg) of hepatitis B virus (HBV) was amplified and cloned into an eucaryotic expression vector. For this aim, HBcAg DNA was amplified with the primers spesific to HBcAg gene of HBV by polymerase chain reaction (PCR). Afterwards, the PCR product of HBcAg gene of HBV was placed into pcDNA 3.1/V5 His-TOPO vector. PCR screening assay was performed to confirm the presence of the HbcAg gene. Furthermore, enzyme digestion assay was performed for the same purpose. In conclusion, the HBcAg gene of HBV was cloned into the eucaryotic expression vector. |
| first_indexed | 2024-04-10T14:20:58Z |
| format | Article |
| id | doaj.art-8f73d515c63a4d2f97d3d96c24bb30b4 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T14:20:58Z |
| publishDate | 2003-06-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-8f73d515c63a4d2f97d3d96c24bb30b42023-02-15T16:09:20ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2003-06-0182144147Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression VectorYasemin BULUT0Aykut ÖZDARENDELİ1Mehmet Ziya DOYMAZ2Zülal AŞÇI TORAMAN3Fırat Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, ELAZIĞFırat Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, ELAZIĞFırat Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, ELAZIĞFırat Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, ELAZIĞIn the presence of this study, the core antigen (HBcAg) of hepatitis B virus (HBV) was amplified and cloned into an eucaryotic expression vector. For this aim, HBcAg DNA was amplified with the primers spesific to HBcAg gene of HBV by polymerase chain reaction (PCR). Afterwards, the PCR product of HBcAg gene of HBV was placed into pcDNA 3.1/V5 His-TOPO vector. PCR screening assay was performed to confirm the presence of the HbcAg gene. Furthermore, enzyme digestion assay was performed for the same purpose. In conclusion, the HBcAg gene of HBV was cloned into the eucaryotic expression vector.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2003-8-2-144-147.pdfHepatitis B virusHBVHBcAgCloning |
| spellingShingle | Yasemin BULUT Aykut ÖZDARENDELİ Mehmet Ziya DOYMAZ Zülal AŞÇI TORAMAN Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Hepatitis B virus HBV HBcAg Cloning |
| title | Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector |
| title_full | Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector |
| title_fullStr | Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector |
| title_full_unstemmed | Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector |
| title_short | Cloning of the Core Antigen (HBcAg) Gene of Hepatitis B Virus into an Eucaryotic Expression Vector |
| title_sort | cloning of the core antigen hbcag gene of hepatitis b virus into an eucaryotic expression vector |
| topic | Hepatitis B virus HBV HBcAg Cloning |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2003-8-2-144-147.pdf |
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