Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa
Background: Pseudomonas aeruginosa (P. aeruginosa), a major gram-negative opportunistic nosocomial pathogen, is notoriously known because of its intrinsic and acquired multiple antibiotic resistances. Different methods are applied in the clinical laboratory to detect Pseudomonas aeruginosa. The aim...
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| Format: | Article |
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Isfahan University of Medical Sciences
2012-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/1783 |
| _version_ | 1797762065708875776 |
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| author | Massumeh Doosti Mehdi Haj Ojagh Faghihi Ali Ramazani Mohammad Reza Saini |
| author_facet | Massumeh Doosti Mehdi Haj Ojagh Faghihi Ali Ramazani Mohammad Reza Saini |
| author_sort | Massumeh Doosti |
| collection | DOAJ |
| description | Background: Pseudomonas aeruginosa (P. aeruginosa), a major gram-negative opportunistic nosocomial pathogen, is notoriously known because of its intrinsic and acquired multiple antibiotic resistances. Different methods are applied in the clinical laboratory to detect Pseudomonas aeruginosa. The aim of this study was to compare culture and molecular diagnostic assays for the detection of Pseudomonas aeruginosa.
Methods: In this study, 70 Pseudomonas aeruginosa strains isolated from different clinical specimens were used. The specimen was examined for the presence of Pseudomonas aeruginosa by plating onto a combination of culture media (Muller Hinton agar, Blood agar, and McConkey agar) and also basic biochemical testes. In addition, from the culture, genomic bacterial DNA was extracted and was amplified employing sequence-specific target, namely the exotoxin A (ETA) gene locus by polymerase chain reaction (PCR) method.
Findings: From the total 300 isolates analyzed in this study, 70 were found by phenotypic tests as P. aeruginosa. The ETA gene was found in 66 isolates (94.3%) by PCR with exotoxin A primers.
Conclusion: These results suggest that the PCR-method mentioned above can be used to provide a specific, rapid, simple, and highly sensitive detection of Pseudomonas aeruginosa in clinical samples.
Keywords: Pseudomonas aeruginosa, Polymerase chain reaction (PCR), Exotoxin A |
| first_indexed | 2024-03-12T19:22:57Z |
| format | Article |
| id | doaj.art-8f73dfe6dfce498caabf189b8910bc2d |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T19:22:57Z |
| publishDate | 2012-08-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-8f73dfe6dfce498caabf189b8910bc2d2023-08-02T05:03:47ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-08-01301921080Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas AeruginosaMassumeh Doosti0Mehdi Haj Ojagh Faghihi1Ali Ramazani2Mohammad Reza Saini3MSc Student, Department of Microbiology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Arak, IranDepartment of Clinical Medicine, Vali-e-Asr Hospital, Zanjan University of Medical Sciences, Zanjan, IranAssistant Professor, Department of Biotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, IranGeneral Practitioner, Center for Disease Control and Prevention, Vice-Chancellor for Health, Zanjan University of Medical Sciences, Zanjan, IranBackground: Pseudomonas aeruginosa (P. aeruginosa), a major gram-negative opportunistic nosocomial pathogen, is notoriously known because of its intrinsic and acquired multiple antibiotic resistances. Different methods are applied in the clinical laboratory to detect Pseudomonas aeruginosa. The aim of this study was to compare culture and molecular diagnostic assays for the detection of Pseudomonas aeruginosa. Methods: In this study, 70 Pseudomonas aeruginosa strains isolated from different clinical specimens were used. The specimen was examined for the presence of Pseudomonas aeruginosa by plating onto a combination of culture media (Muller Hinton agar, Blood agar, and McConkey agar) and also basic biochemical testes. In addition, from the culture, genomic bacterial DNA was extracted and was amplified employing sequence-specific target, namely the exotoxin A (ETA) gene locus by polymerase chain reaction (PCR) method. Findings: From the total 300 isolates analyzed in this study, 70 were found by phenotypic tests as P. aeruginosa. The ETA gene was found in 66 isolates (94.3%) by PCR with exotoxin A primers. Conclusion: These results suggest that the PCR-method mentioned above can be used to provide a specific, rapid, simple, and highly sensitive detection of Pseudomonas aeruginosa in clinical samples. Keywords: Pseudomonas aeruginosa, Polymerase chain reaction (PCR), Exotoxin Ahttp://jims.mui.ac.ir/index.php/jims/article/view/1783 |
| spellingShingle | Massumeh Doosti Mehdi Haj Ojagh Faghihi Ali Ramazani Mohammad Reza Saini Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa مجله دانشکده پزشکی اصفهان |
| title | Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa |
| title_full | Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa |
| title_fullStr | Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa |
| title_full_unstemmed | Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa |
| title_short | Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa |
| title_sort | comparison of conventional culture methods and polymerase chain reaction pcr for specific detection of pseudomonas aeruginosa |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/1783 |
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