Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants
Non-model plants i.e., the species which have one or all of the characters such as long life cycle, difficulty to grow in the laboratory or poor fecundity, have been schemed out of sequencing projects earlier, due to high running cost of Sanger sequencing. Consequently, the information about their g...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-12-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01074/full |
| _version_ | 1811302929995399168 |
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| author | Chibuikem IN Unamba Chibuikem IN Unamba Akshay eNag Ram Kumar Sharma |
| author_facet | Chibuikem IN Unamba Chibuikem IN Unamba Akshay eNag Ram Kumar Sharma |
| author_sort | Chibuikem IN Unamba |
| collection | DOAJ |
| description | Non-model plants i.e., the species which have one or all of the characters such as long life cycle, difficulty to grow in the laboratory or poor fecundity, have been schemed out of sequencing projects earlier, due to high running cost of Sanger sequencing. Consequently, the information about their genomics and key biological processes are inadequate. However, the advent of fast and cost effective next generation sequencing platforms in the recent past has enabled the unearthing of certain characteristic gene structures unique to these species. It has also aided in gaining insight about mechanisms underlying processes of gene expression and secondary metabolism as well as facilitated development of genomic resources for diversity characterization, evolutionary analysis and marker assisted breeding even without prior availability of genomic sequence information. In this review we explore how different Next Gen Sequencing platforms, as well as recent advances in NGS based high throughput genotyping technologies are rewarding efforts on de-novo whole genome/ transcriptome sequencing, development of genome wide sequence based markers resources for improvement of non-model crops that are less costly than phenotyping . |
| first_indexed | 2024-04-13T07:38:07Z |
| format | Article |
| id | doaj.art-8f78debb3ef541fe87b8d7d0bc4c7112 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T07:38:07Z |
| publishDate | 2015-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8f78debb3ef541fe87b8d7d0bc4c71122022-12-22T02:56:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-12-01610.3389/fpls.2015.01074164495Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plantsChibuikem IN Unamba0Chibuikem IN Unamba1Akshay eNag2Ram Kumar Sharma3CSIR-Institute of Himalayan Bioresource Technology, Palampur, INDIAImo State University, Owerri, Imo State, Nigeria.CSIR-Institute of Himalayan Bioresource Technology, Palampur, INDIACSIR-Institute of Himalayan Bioresource Technology, Palampur, INDIANon-model plants i.e., the species which have one or all of the characters such as long life cycle, difficulty to grow in the laboratory or poor fecundity, have been schemed out of sequencing projects earlier, due to high running cost of Sanger sequencing. Consequently, the information about their genomics and key biological processes are inadequate. However, the advent of fast and cost effective next generation sequencing platforms in the recent past has enabled the unearthing of certain characteristic gene structures unique to these species. It has also aided in gaining insight about mechanisms underlying processes of gene expression and secondary metabolism as well as facilitated development of genomic resources for diversity characterization, evolutionary analysis and marker assisted breeding even without prior availability of genomic sequence information. In this review we explore how different Next Gen Sequencing platforms, as well as recent advances in NGS based high throughput genotyping technologies are rewarding efforts on de-novo whole genome/ transcriptome sequencing, development of genome wide sequence based markers resources for improvement of non-model crops that are less costly than phenotyping .http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01074/fullGenomicsnext generation sequencingtranWhole genomeNon model |
| spellingShingle | Chibuikem IN Unamba Chibuikem IN Unamba Akshay eNag Ram Kumar Sharma Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants Frontiers in Plant Science Genomics next generation sequencing tran Whole genome Non model |
| title | Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants |
| title_full | Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants |
| title_fullStr | Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants |
| title_full_unstemmed | Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants |
| title_short | Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants |
| title_sort | next generation sequencing technologies the doorway to the unexplored genomics of non model plants |
| topic | Genomics next generation sequencing tran Whole genome Non model |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01074/full |
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